Restriction map for
pFN21AE4109
(Linear) (Six-base) MAPSORT of: KIEE4109.seq Check: 9947 from: 1 to: 1437
KIEE4109.orf
With 182 enzymes: SgfI *
March 22, 2012 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 220 1421 1437
Size: 220 1201 16
Fragments arranged by size:
1201 220 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1264 1437
Size: 1264 173
AfeI AGC'GCT
Cuts at: 0 604 1437
Size: 604 833
AleI CACnn'nnGTG
Cuts at: 0 415 1437
Size: 415 1022
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 503 1437
Size: 503 934
AlwNI CAG_nnn'CTG
Cuts at: 0 798 1344 1413 1437
Size: 798 546 69 24
Fragments arranged by size:
798 546 69 24
ApaI G_GGCC'C
Cuts at: 0 217 1437
Size: 217 1220
ApaLI G'TGCA_C
Cuts at: 0 219 946 1437
Size: 219 727 491
Fragments arranged by size:
727 491 219
AscI GG'CGCG_CC
Cuts at: 0 868 1437
Size: 868 569
AvaI C'yCGr_G
Cuts at: 0 9 194 628 981 1372 1384 1437
Size: 9 185 434 353 391 12 53
Fragments arranged by size:
434 391 353 185 53 12 9
BanI G'GyrC_C
Cuts at: 0 252 260 1064 1437
Size: 252 8 804 373
Fragments arranged by size:
804 373 252 8
BanII G_rGCy'C
Cuts at: 0 10 217 314 437 1019 1130 1391 1437
Size: 10 207 97 123 582 111 261 46
Fragments arranged by size:
582 261 207 123 111 97 46 10
BbvCI CC'TCA_GC
Cuts at: 0 243 1168 1437
Size: 243 925 269
Fragments arranged by size:
925 269 243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1202 1236 1437
Size: 1202 34 201
Fragments arranged by size:
1202 201 34
BglI GCCn_nnn'nGGC
Cuts at: 0 521 781 983 1437
Size: 521 260 202 454
Fragments arranged by size:
521 454 260 202
BlpI GC'TnA_GC
Cuts at: 0 739 817 973 1437
Size: 739 78 156 464
Fragments arranged by size:
739 464 156 78
Bme1580I G_kGCm'C
Cuts at: 0 115 217 223 263 950 1069 1437
Size: 115 102 6 40 687 119 368
Fragments arranged by size:
687 368 119 115 102 40 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1111 1366 1375 1437
Size: 688 423 255 9 62
Fragments arranged by size:
688 423 255 62 9
Bpu10I CC'TnA_GC
Cuts at: 0 243 718 1168 1437
Size: 243 475 450 269
Fragments arranged by size:
475 450 269 243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1437
Size: 583 854
BsaAI yAC'GTr
Cuts at: 0 837 1098 1437
Size: 837 261 339
Fragments arranged by size:
837 339 261
BsaHI Gr'CG_yC
Cuts at: 0 524 773 1437
Size: 524 249 664
Fragments arranged by size:
664 524 249
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 146 176 1437
Size: 146 30 1261
Fragments arranged by size:
1261 146 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1138 1363 1437
Size: 1138 225 74
Fragments arranged by size:
1138 225 74
BseYI C'CCAG_C
Cuts at: 0 172 208 264 911 1437
Size: 172 36 56 647 526
Fragments arranged by size:
647 526 172 56 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1351 1437
Size: 1096 255 86
Fragments arranged by size:
1096 255 86
BsiEI CG_ry'CG
Cuts at: 0 277 1437
Size: 277 1160
BsiHKAI G_wGCw'C
Cuts at: 0 223 437 950 1019 1130 1136 1391 1437
Size: 223 214 513 69 111 6 255 46
Fragments arranged by size:
513 255 223 214 111 69 46 6
BsmBI CGTCTCn'nnnn_
Cuts at: 0 531 1437
Size: 531 906
Bsp1286I G_dGCh'C
Cuts at: 0 10 115 217 223 263 314 437 950
Size: 10 105 102 6 40 51 123 513
Cuts at: 950 1019 1069 1130 1136 1391 1437
Size: 69 50 61 6 255 46
Fragments arranged by size:
513 255 123 105 102 69 61 51
50 46 40 10 6 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 220 485 1040 1118 1217 1421 1437
Size: 220 265 555 78 99 204 16
Fragments arranged by size:
555 265 220 204 99 78 16
BsrBI CCG'CTC
Cuts at: 0 273 1437
Size: 273 1164
BsrFI r'CCGG_y
Cuts at: 0 414 1437
Size: 414 1023
BssHII G'CGCG_C
Cuts at: 0 722 724 868 1437
Size: 722 2 144 569
Fragments arranged by size:
722 569 144 2
BssSI C'ACGA_G
Cuts at: 0 1018 1059 1129 1437
Size: 1018 41 70 308
Fragments arranged by size:
1018 308 70 41
BstAPI GCAn_nnn'nTGC
Cuts at: 0 798 894 954 1413 1437
Size: 798 96 60 459 24
Fragments arranged by size:
798 459 96 60 24
BstEII G'GTnAC_C
Cuts at: 0 996 1437
Size: 996 441
BstXI CCAn_nnnn'nTGG
Cuts at: 0 87 1437
Size: 87 1350
BstYI r'GATC_y
Cuts at: 0 299 1437
Size: 299 1138
BtgI C'CryG_G
Cuts at: 0 287 927 1437
Size: 287 640 510
Fragments arranged by size:
640 510 287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 762 1227 1437
Size: 762 465 210
Fragments arranged by size:
762 465 210
DraIII CAC_nnn'GTG
Cuts at: 0 933 1437
Size: 933 504
EaeI y'GGCC_r
Cuts at: 0 95 274 284 764 782 1022 1437
Size: 95 179 10 480 18 240 415
Fragments arranged by size:
480 415 240 179 95 18 10
EagI C'GGCC_G
Cuts at: 0 274 1437
Size: 274 1163
EarI CTCTTCn'nnn_
Cuts at: 0 319 1396 1437
Size: 319 1077 41
Fragments arranged by size:
1077 319 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 826 1437
Size: 826 611
EcoICRI GAG'CTC
Cuts at: 0 435 1017 1128 1389 1437
Size: 435 582 111 261 48
Fragments arranged by size:
582 435 261 111 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1111 1264 1366 1375 1437
Size: 688 423 153 102 9 62
Fragments arranged by size:
688 423 153 102 62 9
EcoO109I rG'GnC_Cy
Cuts at: 0 213 1437
Size: 213 1224
FspI TGC'GCA
Cuts at: 0 1036 1045 1437
Size: 1036 9 392
Fragments arranged by size:
1036 392 9
HaeII r_GCGC'y
Cuts at: 0 606 853 1090 1437
Size: 606 247 237 347
Fragments arranged by size:
606 347 247 237
HincII GTy'rAC
Cuts at: 0 939 1437
Size: 939 498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 1437
Size: 554 883
MscI TGG'CCA
Cuts at: 0 286 1437
Size: 286 1151
MslI CAynn'nnrTG
Cuts at: 0 415 760 1081 1437
Size: 415 345 321 356
Fragments arranged by size:
415 356 345 321
MspA1I CmG'CkG
Cuts at: 0 268 282 795 1299 1335 1421 1437
Size: 268 14 513 504 36 86 16
Fragments arranged by size:
513 504 268 86 36 16 14
NotI GC'GGCC_GC
Cuts at: 0 274 1437
Size: 274 1163
NspI r_CATG'y
Cuts at: 0 427 1437
Size: 427 1010
PasI CC'CwG_GG
Cuts at: 0 1260 1437
Size: 1260 177
PflMI CCAn_nnn'nTGG
Cuts at: 0 398 933 1437
Size: 398 535 504
Fragments arranged by size:
535 504 398
PmlI CAC'GTG
Cuts at: 0 837 1437
Size: 837 600
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 1437
Size: 451 32 954
Fragments arranged by size:
954 451 32
PspOMI G'GGCC_C
Cuts at: 0 213 1437
Size: 213 1224
PspXI vC'TCGA_Gb
Cuts at: 0 628 1437
Size: 628 809
PstI C_TGCA'G
Cuts at: 0 482 827 1340 1437
Size: 482 345 513 97
Fragments arranged by size:
513 482 345 97
PvuII CAG'CTG
Cuts at: 0 795 1335 1437
Size: 795 540 102
Fragments arranged by size:
795 540 102
SacI G_AGCT'C
Cuts at: 0 437 1019 1130 1391 1437
Size: 437 582 111 261 46
Fragments arranged by size:
582 437 261 111 46
SapI GCTCTTCn'nnn_
Cuts at: 0 319 1396 1437
Size: 319 1077 41
Fragments arranged by size:
1077 319 41
SbfI CC_TGCA'GG
Cuts at: 0 482 827 1437
Size: 482 345 610
Fragments arranged by size:
610 482 345
SfcI C'TryA_G
Cuts at: 0 478 823 1336 1437
Size: 478 345 513 101
Fragments arranged by size:
513 478 345 101
SgrAI Cr'CCGG_yG
Cuts at: 0 414 1437
Size: 414 1023
SmaI CCC'GGG
Cuts at: 0 11 196 1374 1437
Size: 11 185 1178 63
Fragments arranged by size:
1178 185 63 11
SmlI C'TyrA_G
Cuts at: 0 598 628 1437
Size: 598 30 809
Fragments arranged by size:
809 598 30
SphI G_CATG'C
Cuts at: 0 427 1437
Size: 427 1010
SrfI GCCC'GGGC
Cuts at: 0 11 1437
Size: 11 1426
StuI AGG'CCT
Cuts at: 0 367 407 626 829 1437
Size: 367 40 219 203 608
Fragments arranged by size:
608 367 219 203 40
StyI C'CwwG_G
Cuts at: 0 107 248 428 992 1437
Size: 107 141 180 564 445
Fragments arranged by size:
564 445 180 141 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 171 186 278 1437
Size: 79 92 15 92 1159
Fragments arranged by size:
1159 92 92 79 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 146 178 1437
Size: 146 32 1259
Fragments arranged by size:
1259 146 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 930 1437
Size: 930 507
XhoI C'TCGA_G
Cuts at: 0 628 1437
Size: 628 809
XmaI C'CCGG_G
Cuts at: 0 9 194 1372 1437
Size: 9 185 1178 65
Fragments arranged by size:
1178 185 65 9
XmnI GAAnn'nnTTC
Cuts at: 0 466 1437
Size: 466 971
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AlwNI ApaI ApaLI
AscI AvaI BanI BanII BbvCI BcgI BglI BlpI
Bme1580I BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI
BtgZI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I FspI HaeII HincII MmeI MscI MslI MspA1I
NotI NspI PasI PflMI PmlI PpiI PspOMI PspXI
PstI PvuII SacI SapI SbfI SfcI SgrAI SmaI
SmlI SphI SrfI StuI StyI TaqII TstI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI ApoI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
BplI BsaI BsaBI BsaWI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BstBI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DrdI EcoNI EcoRI EcoRV FalI FseI FspAI
Hin4I HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI
PciI PfoI PmeI PpuMI PshAI PsiI PsrI PvuI
RsrII SacII SalI SanDI ScaI SexAI SfiI SfoI
SgfI SnaBI SpeI SspI SwaI TatI Tth111I XbaI
ZraI