Restriction map for
pFN21AE4107
(Linear) (Six-base) MAPSORT of: KIEE4107.seq Check: 5116 from: 1 to: 1434
KIEE4107.orf
With 182 enzymes: SgfI *
March 22, 2012 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1391 1434
Size: 1391 43
AatII G_ACGT'C
Cuts at: 0 917 1434
Size: 917 517
AccI GT'mk_AC
Cuts at: 0 1406 1434
Size: 1406 28
Acc65I G'GTAC_C
Cuts at: 0 362 515 1434
Size: 362 153 919
Fragments arranged by size:
919 362 153
AfeI AGC'GCT
Cuts at: 0 154 1434
Size: 154 1280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1156 1434
Size: 1156 278
AlwNI CAG_nnn'CTG
Cuts at: 0 44 165 573 648 1434
Size: 44 121 408 75 786
Fragments arranged by size:
786 408 121 75 44
ApaI G_GGCC'C
Cuts at: 0 502 1033 1434
Size: 502 531 401
Fragments arranged by size:
531 502 401
AscI GG'CGCG_CC
Cuts at: 0 826 1249 1434
Size: 826 423 185
Fragments arranged by size:
826 423 185
AvaI C'yCGr_G
Cuts at: 0 628 1032 1434
Size: 628 404 402
Fragments arranged by size:
628 404 402
AvrII C'CTAG_G
Cuts at: 0 4 1434
Size: 4 1430
BanI G'GyrC_C
Cuts at: 0 118 217 362 515 532 616 964 1434
Size: 118 99 145 153 17 84 348 470
Fragments arranged by size:
470 348 153 145 118 99 84 17
BanII G_rGCy'C
Cuts at: 0 502 628 1033 1434
Size: 502 126 405 401
Fragments arranged by size:
502 405 401 126
BbeI G_GCGC'C
Cuts at: 0 620 968 1434
Size: 620 348 466
Fragments arranged by size:
620 466 348
BbsI GAAGACnn'nnnn_
Cuts at: 0 336 1237 1434
Size: 336 901 197
Fragments arranged by size:
901 336 197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 930 964 1434
Size: 930 34 470
Fragments arranged by size:
930 470 34
BglI GCCn_nnn'nGGC
Cuts at: 0 598 748 1037 1089 1259 1434
Size: 598 150 289 52 170 175
Fragments arranged by size:
598 289 175 170 150 52
Bme1580I G_kGCm'C
Cuts at: 0 220 502 535 1033 1135 1434
Size: 220 282 33 498 102 299
Fragments arranged by size:
498 299 282 220 102 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 304 703 721 1434
Size: 133 171 399 18 713
Fragments arranged by size:
713 399 171 133 18
BsaHI Gr'CG_yC
Cuts at: 0 617 914 965 1124 1186 1434
Size: 617 297 51 159 62 248
Fragments arranged by size:
617 297 248 159 62 51
BsaWI w'CCGG_w
Cuts at: 0 1421 1434
Size: 1421 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1321 1434
Size: 1321 113
BseYI C'CCAG_C
Cuts at: 0 258 711 1026 1434
Size: 258 453 315 408
Fragments arranged by size:
453 408 315 258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 250 397 877 1363 1369 1434
Size: 226 24 147 480 486 6 65
Fragments arranged by size:
486 480 226 147 65 24 6
BsiEI CG_ry'CG
Cuts at: 0 593 727 1002 1167 1171 1434
Size: 593 134 275 165 4 263
Fragments arranged by size:
593 275 263 165 134 4
BsiHKAI G_wGCw'C
Cuts at: 0 149 1076 1434
Size: 149 927 358
Fragments arranged by size:
927 358 149
Bsp1286I G_dGCh'C
Cuts at: 0 149 220 502 535 628 1033 1076 1135
Size: 149 71 282 33 93 405 43 59
Cuts at: 1135 1434
Size: 299
Fragments arranged by size:
405 299 282 149 93 71 59 43
33
BspEI T'CCGG_A
Cuts at: 0 1421 1434
Size: 1421 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1346 1391 1434
Size: 1346 45 43
Fragments arranged by size:
1346 45 43
BsrBI CCG'CTC
Cuts at: 0 747 1434
Size: 747 687
BsrFI r'CCGG_y
Cuts at: 0 783 1188 1312 1434
Size: 783 405 124 122
Fragments arranged by size:
783 405 124 122
BssHII G'CGCG_C
Cuts at: 0 474 476 719 756 808 826 1150 1249
Size: 474 2 243 37 52 18 324 99
Cuts at: 1249 1434
Size: 185
Fragments arranged by size:
474 324 243 185 99 52 37 18
2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 165 648 1434
Size: 165 483 786
Fragments arranged by size:
786 483 165
BstEII G'GTnAC_C
Cuts at: 0 72 1059 1434
Size: 72 987 375
Fragments arranged by size:
987 375 72
BstXI CCAn_nnnn'nTGG
Cuts at: 0 192 1434
Size: 192 1242
BstZ17I GTA'TAC
Cuts at: 0 1407 1434
Size: 1407 27
BtgI C'CryG_G
Cuts at: 0 593 737 831 839 970 1127 1171 1434
Size: 593 144 94 8 131 157 44 263
Fragments arranged by size:
593 263 157 144 131 94 44 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 203 1434
Size: 203 1231
BtsI GCAGTG_nn'
Cuts at: 0 1133 1434
Size: 1133 301
DraIII CAC_nnn'GTG
Cuts at: 0 611 1434
Size: 611 823
EaeI y'GGCC_r
Cuts at: 0 422 461 590 599 724 740 845 1038
Size: 422 39 129 9 125 16 105 193
Cuts at: 1038 1164 1190 1434
Size: 126 26 244
Fragments arranged by size:
422 244 193 129 126 125 105 39
26 16 9
EagI C'GGCC_G
Cuts at: 0 590 724 1164 1434
Size: 590 134 440 270
Fragments arranged by size:
590 440 270 134
EarI CTCTTCn'nnn_
Cuts at: 0 1081 1434
Size: 1081 353
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1264 1434
Size: 1264 170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 304 703 721 1434
Size: 133 171 399 18 713
Fragments arranged by size:
713 399 171 133 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 8 859 1434
Size: 8 851 575
Fragments arranged by size:
851 575 8
EcoO109I rG'GnC_Cy
Cuts at: 0 29 546 1434
Size: 29 517 888
Fragments arranged by size:
888 517 29
HaeII r_GCGC'y
Cuts at: 0 156 620 754 929 968 1434
Size: 156 464 134 175 39 466
Fragments arranged by size:
466 464 175 156 134 39
HincII GTy'rAC
Cuts at: 0 918 1434
Size: 918 516
KasI G'GCGC_C
Cuts at: 0 616 964 1434
Size: 616 348 470
Fragments arranged by size:
616 470 348
KpnI G_GTAC'C
Cuts at: 0 366 519 1434
Size: 366 153 915
Fragments arranged by size:
915 366 153
MscI TGG'CCA
Cuts at: 0 424 1040 1434
Size: 424 616 394
Fragments arranged by size:
616 424 394
MspA1I CmG'CkG
Cuts at: 0 115 340 792 801 841 891 1026 1129
Size: 115 225 452 9 40 50 135 103
Cuts at: 1129 1173 1199 1344 1434
Size: 44 26 145 90
Fragments arranged by size:
452 225 145 135 115 103 90 50
44 40 26 9
NaeI GCC'GGC
Cuts at: 0 1190 1314 1434
Size: 1190 124 120
Fragments arranged by size:
1190 124 120
NarI GG'CG_CC
Cuts at: 0 617 965 1434
Size: 617 348 469
Fragments arranged by size:
617 469 348
NgoMIV G'CCGG_C
Cuts at: 0 1188 1312 1434
Size: 1188 124 122
Fragments arranged by size:
1188 124 122
NotI GC'GGCC_GC
Cuts at: 0 724 1434
Size: 724 710
NspI r_CATG'y
Cuts at: 0 474 1434
Size: 474 960
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 1434
Size: 699 735
PfoI T'CCnGG_A
Cuts at: 0 281 1434
Size: 281 1153
PpuMI rG'GwC_Cy
Cuts at: 0 29 1434
Size: 29 1405
PspOMI G'GGCC_C
Cuts at: 0 498 1029 1434
Size: 498 531 405
Fragments arranged by size:
531 498 405
PstI C_TGCA'G
Cuts at: 0 320 452 569 674 806 863 1343 1434
Size: 320 132 117 105 132 57 480 91
Fragments arranged by size:
480 320 132 132 117 105 91 57
PvuII CAG'CTG
Cuts at: 0 115 792 801 1026 1434
Size: 115 677 9 225 408
Fragments arranged by size:
677 408 225 115 9
SacII CC_GC'GG
Cuts at: 0 842 1130 1174 1434
Size: 842 288 44 260
Fragments arranged by size:
842 288 260 44
SapI GCTCTTCn'nnn_
Cuts at: 0 1081 1434
Size: 1081 353
SexAI A'CCwGG_T
Cuts at: 0 662 1361 1434
Size: 662 699 73
Fragments arranged by size:
699 662 73
SfcI C'TryA_G
Cuts at: 0 316 448 565 670 802 859 1284 1339
Size: 316 132 117 105 132 57 425 55
Cuts at: 1339 1434
Size: 95
Fragments arranged by size:
425 316 132 132 117 105 95 57
55
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 598 1037 1434
Size: 598 439 397
Fragments arranged by size:
598 439 397
SfoI GGC'GCC
Cuts at: 0 618 966 1434
Size: 618 348 468
Fragments arranged by size:
618 468 348
StuI AGG'CCT
Cuts at: 0 706 865 1434
Size: 706 159 569
Fragments arranged by size:
706 569 159
StyI C'CwwG_G
Cuts at: 0 4 59 1434
Size: 4 55 1375
Fragments arranged by size:
1375 55 4
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 91 1434
Size: 91 1343
TatI w'GTAC_w
Cuts at: 0 368 1434
Size: 368 1066
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 779 811 1434
Size: 779 32 623
Fragments arranged by size:
779 623 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 57 1434
Size: 57 1377
ZraI GAC'GTC
Cuts at: 0 915 1434
Size: 915 519
Enzymes that do cut:
AarI AatII AccI Acc65I AfeI AlfI AlwNI ApaI
AscI AvaI AvrII BanI BanII BbeI BbsI BcgI
BglI Bme1580I BpmI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII
BstAPI BstEII BstXI BstZ17I BtgI BtgZI BtsI DraIII
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII
HincII KasI KpnI MscI MspA1I NaeI NarI NgoMIV
NotI NspI PflMI PfoI PpuMI PspOMI PstI PvuII
SacII SapI SexAI SfcI SfiI SfoI StuI StyI
TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
AclI AcuI AflII AflIII AgeI AhdI AleI AloI
ApaLI ApoI AseI AsiSI BaeI BamHI BbvCI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsiWI BsmI
BsmBI BspHI BsrDI BsrGI BssSI BstBI BstYI Bsu36I
ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI MfeI MluI
MmeI MslI NcoI NdeI NheI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XhoI XmaI XmnI