Restriction map for
pFN21AE4101
(Linear) (Six-base) MAPSORT of: KIEE4101.seq Check: 8017 from: 1 to: 1413
KIEE4101.orf
With 182 enzymes: SgfI *
July 3, 2012 18:34 ..
AccI GT'mk_AC
Cuts at: 0 458 1413
Size: 458 955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 998 1337 1413
Size: 509 489 339 76
Fragments arranged by size:
509 489 339 76
AflIII A'CryG_T
Cuts at: 0 303 1413
Size: 303 1110
AlwNI CAG_nnn'CTG
Cuts at: 0 1183 1354 1413
Size: 1183 171 59
Fragments arranged by size:
1183 171 59
ApoI r'AATT_y
Cuts at: 0 159 400 507 1413
Size: 159 241 107 906
Fragments arranged by size:
906 241 159 107
AseI AT'TA_AT
Cuts at: 0 379 1413
Size: 379 1034
BanII G_rGCy'C
Cuts at: 0 825 1413
Size: 825 588
BbsI GAAGACnn'nnnn_
Cuts at: 0 1058 1413
Size: 1058 355
BmtI G_CTAG'C
Cuts at: 0 469 1413
Size: 469 944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 1413
Size: 369 1044
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 1088 1413
Size: 299 789 325
Fragments arranged by size:
789 325 299
BseYI C'CCAG_C
Cuts at: 0 518 831 1413
Size: 518 313 582
Fragments arranged by size:
582 518 313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 1129 1413
Size: 573 556 284
Fragments arranged by size:
573 556 284
BsiHKAI G_wGCw'C
Cuts at: 0 1064 1413
Size: 1064 349
BsmI GAATG_Cn'
Cuts at: 0 1224 1413
Size: 1224 189
BsmBI CGTCTCn'nnnn_
Cuts at: 0 18 1413
Size: 18 1395
Bsp1286I G_dGCh'C
Cuts at: 0 825 1064 1413
Size: 825 239 349
Fragments arranged by size:
825 349 239
BspHI T'CATG_A
Cuts at: 0 528 893 1413
Size: 528 365 520
Fragments arranged by size:
528 520 365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 161 444 1413
Size: 161 283 969
Fragments arranged by size:
969 283 161
BsrDI GCAATG_nn'
Cuts at: 0 810 1413
Size: 810 603
BstYI r'GATC_y
Cuts at: 0 1156 1413
Size: 1156 257
BtgI C'CryG_G
Cuts at: 0 113 1127 1413
Size: 113 1014 286
Fragments arranged by size:
1014 286 113
BtsI GCAGTG_nn'
Cuts at: 0 1377 1413
Size: 1377 36
DraI TTT'AAA
Cuts at: 0 132 1413
Size: 132 1281
DrdI GACnn_nn'nnGTC
Cuts at: 0 628 848 1413
Size: 628 220 565
Fragments arranged by size:
628 565 220
EaeI y'GGCC_r
Cuts at: 0 1074 1413
Size: 1074 339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 509 998 1337 1413
Size: 369 140 489 339 76
Fragments arranged by size:
489 369 339 140 76
EcoO109I rG'GnC_Cy
Cuts at: 0 18 54 1413
Size: 18 36 1359
Fragments arranged by size:
1359 36 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 430 1304 1413
Size: 430 874 109
Fragments arranged by size:
874 430 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 812 844 1413
Size: 812 32 569
Fragments arranged by size:
812 569 32
HincII GTy'rAC
Cuts at: 0 759 853 1194 1413
Size: 759 94 341 219
Fragments arranged by size:
759 341 219 94
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 354 543 1142 1149 1413
Size: 276 78 189 599 7 264
Fragments arranged by size:
599 276 264 189 78 7
MscI TGG'CCA
Cuts at: 0 1076 1413
Size: 1076 337
MslI CAynn'nnrTG
Cuts at: 0 308 1413
Size: 308 1105
MspA1I CmG'CkG
Cuts at: 0 1113 1413
Size: 1113 300
NcoI C'CATG_G
Cuts at: 0 113 1127 1413
Size: 113 1014 286
Fragments arranged by size:
1014 286 113
NheI G'CTAG_C
Cuts at: 0 465 1413
Size: 465 948
NspI r_CATG'y
Cuts at: 0 307 1413
Size: 307 1106
PacI TTA_AT'TAA
Cuts at: 0 383 1413
Size: 383 1030
PciI A'CATG_T
Cuts at: 0 303 1413
Size: 303 1110
PpuMI rG'GwC_Cy
Cuts at: 0 54 1413
Size: 54 1359
PvuII CAG'CTG
Cuts at: 0 1113 1413
Size: 1113 300
SmlI C'TyrA_G
Cuts at: 0 314 1103 1413
Size: 314 789 310
Fragments arranged by size:
789 314 310
SspI AAT'ATT
Cuts at: 0 109 1413
Size: 109 1304
StyI C'CwwG_G
Cuts at: 0 113 737 1127 1296 1413
Size: 113 624 390 169 117
Fragments arranged by size:
624 390 169 117 113
TatI w'GTAC_w
Cuts at: 0 41 919 1413
Size: 41 878 494
Fragments arranged by size:
878 494 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 290 322 1413
Size: 290 32 1091
Fragments arranged by size:
1091 290 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 218 1125 1413
Size: 218 907 288
Fragments arranged by size:
907 288 218
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApoI AseI BanII BbsI
BmtI BpmI BpuEI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BstYI BtgI BtsI DraI
DrdI EaeI Eco57MI EcoO109I FalI Hin4I HincII MmeI
MscI MslI MspA1I NcoI NheI NspI PacI PciI
PpuMI PvuII SmlI SspI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BsiEI BsiWI BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII EagI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TaqII Tth111I XbaI
XhoI XmaI XmnI ZraI