Restriction map for
pFN21AE4095
(Linear) (Six-base) MAPSORT of: KIEE4095.seq Check: 889 from: 1 to: 1404
KIEE4095.orf
With 182 enzymes: SgfI *
July 3, 2012 18:33 ..
AccI GT'mk_AC
Cuts at: 0 4 1404
Size: 4 1400
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 760 934 1404
Size: 586 174 174 470
Fragments arranged by size:
586 470 174 174
AlwNI CAG_nnn'CTG
Cuts at: 0 128 255 564 1166 1404
Size: 128 127 309 602 238
Fragments arranged by size:
602 309 238 128 127
ApaI G_GGCC'C
Cuts at: 0 187 1404
Size: 187 1217
ApoI r'AATT_y
Cuts at: 0 847 1404
Size: 847 557
BanII G_rGCy'C
Cuts at: 0 187 750 1404
Size: 187 563 654
Fragments arranged by size:
654 563 187
BbsI GAAGACnn'nnnn_
Cuts at: 0 1218 1404
Size: 1218 186
BbvCI CC'TCA_GC
Cuts at: 0 392 631 1404
Size: 392 239 773
Fragments arranged by size:
773 392 239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 666 700 1404
Size: 666 34 704
Fragments arranged by size:
704 666 34
BglII A'GATC_T
Cuts at: 0 12 1001 1404
Size: 12 989 403
Fragments arranged by size:
989 403 12
Bme1580I G_kGCm'C
Cuts at: 0 98 187 1404
Size: 98 89 1217
Fragments arranged by size:
1217 98 89
BmgBI CAC'GTC
Cuts at: 0 1197 1401 1404
Size: 1197 204 3
Fragments arranged by size:
1197 204 3
BmrI ACTGGGnnnn_n'
Cuts at: 0 70 1035 1404
Size: 70 965 369
Fragments arranged by size:
965 369 70
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 394 430 1404
Size: 394 36 974
Fragments arranged by size:
974 394 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 366 400 574 1404
Size: 189 177 34 174 830
Fragments arranged by size:
830 189 177 174 34
Bpu10I CC'TnA_GC
Cuts at: 0 392 458 631 673 1404
Size: 392 66 173 42 731
Fragments arranged by size:
731 392 173 66 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 751 1404
Size: 687 64 653
Fragments arranged by size:
687 653 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 1176 1404
Size: 1176 228
BsaWI w'CCGG_w
Cuts at: 0 242 1404
Size: 242 1162
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 409 589 758 1404
Size: 109 300 180 169 646
Fragments arranged by size:
646 300 180 169 109
BseYI C'CCAG_C
Cuts at: 0 128 656 873 990 1404
Size: 128 528 217 117 414
Fragments arranged by size:
528 414 217 128 117
BsmI GAATG_Cn'
Cuts at: 0 1255 1404
Size: 1255 149
Bsp1286I G_dGCh'C
Cuts at: 0 98 187 750 1404
Size: 98 89 563 654
Fragments arranged by size:
654 563 98 89
BssSI C'ACGA_G
Cuts at: 0 895 1404
Size: 895 509
BstBI TT'CG_AA
Cuts at: 0 722 1404
Size: 722 682
BstXI CCAn_nnnn'nTGG
Cuts at: 0 358 1404
Size: 358 1046
BstYI r'GATC_y
Cuts at: 0 12 909 1001 1404
Size: 12 897 92 403
Fragments arranged by size:
897 403 92 12
Bsu36I CC'TnA_GG
Cuts at: 0 28 121 1321 1404
Size: 28 93 1200 83
Fragments arranged by size:
1200 93 83 28
BtgI C'CryG_G
Cuts at: 0 357 1404
Size: 357 1047
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 213 1404
Size: 213 1191
BtsI GCAGTG_nn'
Cuts at: 0 299 1404
Size: 299 1105
DraI TTT'AAA
Cuts at: 0 1142 1404
Size: 1142 262
DraIII CAC_nnn'GTG
Cuts at: 0 89 1404
Size: 89 1315
EaeI y'GGCC_r
Cuts at: 0 221 1404
Size: 221 1183
EarI CTCTTCn'nnn_
Cuts at: 0 566 602 910 1404
Size: 566 36 308 494
Fragments arranged by size:
566 494 308 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 366 400 574 586 760 934 1404
Size: 189 177 34 174 12 174 174 470
Fragments arranged by size:
470 189 177 174 174 174 34 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 1184 1404
Size: 31 1153 220
Fragments arranged by size:
1153 220 31
EcoO109I rG'GnC_Cy
Cuts at: 0 173 183 1119 1317 1404
Size: 173 10 936 198 87
Fragments arranged by size:
936 198 173 87 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 734 1404
Size: 734 670
HaeII r_GCGC'y
Cuts at: 0 313 341 1404
Size: 313 28 1063
Fragments arranged by size:
1063 313 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1111 1143 1225 1257 1404
Size: 1111 32 82 32 147
Fragments arranged by size:
1111 147 82 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 1404
Size: 884 520
MscI TGG'CCA
Cuts at: 0 223 1404
Size: 223 1181
MslI CAynn'nnrTG
Cuts at: 0 550 826 1404
Size: 550 276 578
Fragments arranged by size:
578 550 276
NcoI C'CATG_G
Cuts at: 0 357 1404
Size: 357 1047
PfoI T'CCnGG_A
Cuts at: 0 416 1229 1404
Size: 416 813 175
Fragments arranged by size:
813 416 175
PpuMI rG'GwC_Cy
Cuts at: 0 173 1119 1404
Size: 173 946 285
Fragments arranged by size:
946 285 173
PspOMI G'GGCC_C
Cuts at: 0 183 1404
Size: 183 1221
PstI C_TGCA'G
Cuts at: 0 260 1404
Size: 260 1144
SfcI C'TryA_G
Cuts at: 0 5 256 1287 1404
Size: 5 251 1031 117
Fragments arranged by size:
1031 251 117 5
SmlI C'TyrA_G
Cuts at: 0 702 730 1404
Size: 702 28 674
Fragments arranged by size:
702 674 28
StuI AGG'CCT
Cuts at: 0 1326 1404
Size: 1326 78
StyI C'CwwG_G
Cuts at: 0 176 305 357 1404
Size: 176 129 52 1047
Fragments arranged by size:
1047 176 129 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1066 1404
Size: 1066 338
TatI w'GTAC_w
Cuts at: 0 465 1404
Size: 465 939
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApoI BanII BbsI BbvCI
BcgI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaWI BseRI BseYI BsmI Bsp1286I BssSI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I MmeI MscI MslI NcoI PfoI PpuMI PspOMI
PstI SfcI SmlI StuI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BciVI BclI
BfrBI BglI BlpI BmtI BsaAI BsaBI BsaHI BsaXI
BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstZ17I
ClaI CspCI DrdI EagI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI