Restriction map for
pFN21AE4091
(Linear) (Six-base) MAPSORT of: KIEE4091.seq Check: 9905 from: 1 to: 1374
KIEE4091.orf
With 182 enzymes: SgfI *
March 22, 2012 17:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1124 1374
Size: 1124 250
AlwNI CAG_nnn'CTG
Cuts at: 0 123 499 847 1140 1374
Size: 123 376 348 293 234
Fragments arranged by size:
376 348 293 234 123
ApaI G_GGCC'C
Cuts at: 0 1122 1374
Size: 1122 252
ApoI r'AATT_y
Cuts at: 0 630 1374
Size: 630 744
BamHI G'GATC_C
Cuts at: 0 376 1374
Size: 376 998
BanII G_rGCy'C
Cuts at: 0 14 449 1122 1374
Size: 14 435 673 252
Fragments arranged by size:
673 435 252 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 545 579 954 988 1374
Size: 545 34 375 34 386
Fragments arranged by size:
545 386 375 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 25 37 1374
Size: 25 12 1337
Fragments arranged by size:
1337 25 12
Bme1580I G_kGCm'C
Cuts at: 0 568 761 1122 1374
Size: 568 193 361 252
Fragments arranged by size:
568 361 252 193
BmrI ACTGGGnnnn_n'
Cuts at: 0 250 1374
Size: 250 1124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 223 1374
Size: 223 1151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 831 1019 1374
Size: 831 188 355
Fragments arranged by size:
831 355 188
BsaI GGTCTCn'nnnn_
Cuts at: 0 197 1017 1374
Size: 197 820 357
Fragments arranged by size:
820 357 197
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 182 215 1374
Size: 182 33 1159
Fragments arranged by size:
1159 182 33
BseYI C'CCAG_C
Cuts at: 0 13 116 326 1374
Size: 13 103 210 1048
Fragments arranged by size:
1048 210 103 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1374
Size: 1153 221
BsiEI CG_ry'CG
Cuts at: 0 44 1374
Size: 44 1330
BsiHKAI G_wGCw'C
Cuts at: 0 449 1374
Size: 449 925
Bsp1286I G_dGCh'C
Cuts at: 0 14 449 568 761 1122 1374
Size: 14 435 119 193 361 252
Fragments arranged by size:
435 361 252 193 119 14
BsrDI GCAATG_nn'
Cuts at: 0 647 987 1374
Size: 647 340 387
Fragments arranged by size:
647 387 340
BsrFI r'CCGG_y
Cuts at: 0 1128 1374
Size: 1128 246
BstAPI GCAn_nnn'nTGC
Cuts at: 0 499 1140 1374
Size: 499 641 234
Fragments arranged by size:
641 499 234
BstEII G'GTnAC_C
Cuts at: 0 1239 1374
Size: 1239 135
BstYI r'GATC_y
Cuts at: 0 376 1374
Size: 376 998
BtgI C'CryG_G
Cuts at: 0 35 1289 1374
Size: 35 1254 85
Fragments arranged by size:
1254 85 35
BtsI GCAGTG_nn'
Cuts at: 0 800 1374
Size: 800 574
DrdI GACnn_nn'nnGTC
Cuts at: 0 1237 1374
Size: 1237 137
EaeI y'GGCC_r
Cuts at: 0 41 90 390 1179 1374
Size: 41 49 300 789 195
Fragments arranged by size:
789 300 195 49 41
EagI C'GGCC_G
Cuts at: 0 41 1374
Size: 41 1333
EarI CTCTTCn'nnn_
Cuts at: 0 55 584 1374
Size: 55 529 790
Fragments arranged by size:
790 529 55
EcoICRI GAG'CTC
Cuts at: 0 447 1374
Size: 447 927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 831 1019 1124 1374
Size: 831 188 105 250
Fragments arranged by size:
831 250 188 105
EcoO109I rG'GnC_Cy
Cuts at: 0 1119 1230 1374
Size: 1119 111 144
Fragments arranged by size:
1119 144 111
EcoRI G'AATT_C
Cuts at: 0 630 1374
Size: 630 744
EcoRV GAT'ATC
Cuts at: 0 264 1374
Size: 264 1110
HaeII r_GCGC'y
Cuts at: 0 20 1374
Size: 20 1354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1261 1293 1374
Size: 1261 32 81
Fragments arranged by size:
1261 81 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 404 881 1374
Size: 333 71 477 493
Fragments arranged by size:
493 477 333 71
MscI TGG'CCA
Cuts at: 0 392 1181 1374
Size: 392 789 193
Fragments arranged by size:
789 392 193
MspA1I CmG'CkG
Cuts at: 0 37 1203 1374
Size: 37 1166 171
Fragments arranged by size:
1166 171 37
NcoI C'CATG_G
Cuts at: 0 1289 1374
Size: 1289 85
NotI GC'GGCC_GC
Cuts at: 0 41 1374
Size: 41 1333
NspI r_CATG'y
Cuts at: 0 493 975 1089 1374
Size: 493 482 114 285
Fragments arranged by size:
493 482 285 114
PasI CC'CwG_GG
Cuts at: 0 568 1374
Size: 568 806
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 1115 1188 1374
Size: 315 800 73 186
Fragments arranged by size:
800 315 186 73
PfoI T'CCnGG_A
Cuts at: 0 1226 1374
Size: 1226 148
PpuMI rG'GwC_Cy
Cuts at: 0 1230 1374
Size: 1230 144
PshAI GACnn'nnGTC
Cuts at: 0 881 1374
Size: 881 493
PspOMI G'GGCC_C
Cuts at: 0 1118 1374
Size: 1118 256
PstI C_TGCA'G
Cuts at: 0 186 1374
Size: 186 1188
PvuII CAG'CTG
Cuts at: 0 1203 1374
Size: 1203 171
SacI G_AGCT'C
Cuts at: 0 449 1374
Size: 449 925
SacII CC_GC'GG
Cuts at: 0 38 1374
Size: 38 1336
SapI GCTCTTCn'nnn_
Cuts at: 0 55 1374
Size: 55 1319
SexAI A'CCwGG_T
Cuts at: 0 827 1374
Size: 827 547
SfcI C'TryA_G
Cuts at: 0 182 503 1374
Size: 182 321 871
Fragments arranged by size:
871 321 182
SphI G_CATG'C
Cuts at: 0 493 975 1374
Size: 493 482 399
Fragments arranged by size:
493 482 399
StyI C'CwwG_G
Cuts at: 0 1187 1235 1289 1374
Size: 1187 48 54 85
Fragments arranged by size:
1187 85 54 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 767 799 1148 1180 1374
Size: 767 32 349 32 194
Fragments arranged by size:
767 349 194 32 32
XbaI T'CTAG_A
Cuts at: 0 624 1374
Size: 624 750
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 312 532 1374
Size: 312 220 842
Fragments arranged by size:
842 312 220
XmnI GAAnn'nnTTC
Cuts at: 0 130 1374
Size: 130 1244
Enzymes that do cut:
AcuI AlwNI ApaI ApoI BamHI BanII BcgI BglI
Bme1580I BmrI BplI BpmI BsaI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BsrDI BsrFI BstAPI BstEII BstYI
BtgI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV HaeII Hin4I MmeI MscI MspA1I
NcoI NotI NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PstI PvuII SacI SacII SapI SexAI SfcI
SphI StyI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI
BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EciI EcoNI FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NdeI NgoMIV NheI NruI NsiI PacI PciI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TatI Tth111I XhoI XmaI ZraI