Restriction map for
pFN21AE4088
(Linear) (Six-base) MAPSORT of: KIEE4088.seq Check: 8351 from: 1 to: 1356
KIEE4088.orf
With 182 enzymes: SgfI *
July 3, 2012 18:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1356
Size: 718 638
AlwNI CAG_nnn'CTG
Cuts at: 0 500 1356
Size: 500 856
ApaI G_GGCC'C
Cuts at: 0 821 1356
Size: 821 535
ApoI r'AATT_y
Cuts at: 0 3 1356
Size: 3 1353
BanII G_rGCy'C
Cuts at: 0 419 809 821 999 1356
Size: 419 390 12 178 357
Fragments arranged by size:
419 390 357 178 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 15 175 1356
Size: 15 160 1181
Fragments arranged by size:
1181 160 15
BbvCI CC'TCA_GC
Cuts at: 0 398 1356
Size: 398 958
BclI T'GATC_A
Cuts at: 0 1275 1356
Size: 1275 81
Bme1580I G_kGCm'C
Cuts at: 0 36 95 821 1356
Size: 36 59 726 535
Fragments arranged by size:
726 535 59 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 67 1356
Size: 67 1289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1356
Size: 718 638
Bpu10I CC'TnA_GC
Cuts at: 0 398 998 1356
Size: 398 600 358
Fragments arranged by size:
600 398 358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1356
Size: 929 427
BsaBI GATnn'nnATC
Cuts at: 0 1274 1356
Size: 1274 82
BsaWI w'CCGG_w
Cuts at: 0 362 1356
Size: 362 994
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 427 1135 1313 1356
Size: 280 147 708 178 43
Fragments arranged by size:
708 280 178 147 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 1356
Size: 833 523
BsiHKAI G_wGCw'C
Cuts at: 0 157 346 362 419 809 1356
Size: 157 189 16 57 390 547
Fragments arranged by size:
547 390 189 157 57 16
BsmI GAATG_Cn'
Cuts at: 0 166 1356
Size: 166 1190
Bsp1286I G_dGCh'C
Cuts at: 0 36 95 157 346 362 419 809 821
Size: 36 59 62 189 16 57 390 12
Cuts at: 821 999 1356
Size: 178 357
Fragments arranged by size:
390 357 189 178 62 59 57 36
16 12
BsrDI GCAATG_nn'
Cuts at: 0 224 1356
Size: 224 1132
BsrFI r'CCGG_y
Cuts at: 0 104 1356
Size: 104 1252
BstAPI GCAn_nnn'nTGC
Cuts at: 0 145 1356
Size: 145 1211
BstXI CCAn_nnnn'nTGG
Cuts at: 0 742 1356
Size: 742 614
BstYI r'GATC_y
Cuts at: 0 693 1356
Size: 693 663
BtsI GCAGTG_nn'
Cuts at: 0 1161 1356
Size: 1161 195
EarI CTCTTCn'nnn_
Cuts at: 0 572 1087 1356
Size: 572 515 269
Fragments arranged by size:
572 515 269
EcoICRI GAG'CTC
Cuts at: 0 417 807 1356
Size: 417 390 549
Fragments arranged by size:
549 417 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1356
Size: 718 638
EcoO109I rG'GnC_Cy
Cuts at: 0 817 1356
Size: 817 539
EcoRI G'AATT_C
Cuts at: 0 3 1356
Size: 3 1353
EcoRV GAT'ATC
Cuts at: 0 960 1356
Size: 960 396
HindIII A'AGCT_T
Cuts at: 0 449 1356
Size: 449 907
MfeI C'AATT_G
Cuts at: 0 1305 1356
Size: 1305 51
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 912 1356
Size: 371 541 444
Fragments arranged by size:
541 444 371
MslI CAynn'nnrTG
Cuts at: 0 52 761 1356
Size: 52 709 595
Fragments arranged by size:
709 595 52
NaeI GCC'GGC
Cuts at: 0 106 1356
Size: 106 1250
NdeI CA'TA_TG
Cuts at: 0 1037 1356
Size: 1037 319
NgoMIV G'CCGG_C
Cuts at: 0 104 1356
Size: 104 1252
PflMI CCAn_nnn'nTGG
Cuts at: 0 389 500 1356
Size: 389 111 856
Fragments arranged by size:
856 389 111
PfoI T'CCnGG_A
Cuts at: 0 1235 1356
Size: 1235 121
PspOMI G'GGCC_C
Cuts at: 0 817 1356
Size: 817 539
PstI C_TGCA'G
Cuts at: 0 875 1168 1356
Size: 875 293 188
Fragments arranged by size:
875 293 188
SacI G_AGCT'C
Cuts at: 0 419 809 1356
Size: 419 390 547
Fragments arranged by size:
547 419 390
SapI GCTCTTCn'nnn_
Cuts at: 0 572 1356
Size: 572 784
SfcI C'TryA_G
Cuts at: 0 871 1164 1344 1356
Size: 871 293 180 12
Fragments arranged by size:
871 293 180 12
SmlI C'TyrA_G
Cuts at: 0 944 1356
Size: 944 412
SspI AAT'ATT
Cuts at: 0 1021 1356
Size: 1021 335
StuI AGG'CCT
Cuts at: 0 1332 1356
Size: 1332 24
Tth111I GACn'n_nGTC
Cuts at: 0 73 1356
Size: 73 1283
XmnI GAAnn'nnTTC
Cuts at: 0 304 980 1356
Size: 304 676 376
Fragments arranged by size:
676 376 304
Enzymes that do cut:
AcuI AlwNI ApaI ApoI BanII BbsI BbvCI BclI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI BsaWI BseRI
BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BstAPI BstXI
BstYI BtsI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
HindIII MfeI MmeI MslI NaeI NdeI NgoMIV PflMI
PfoI PspOMI PstI SacI SapI SfcI SmlI SspI
StuI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BsaI BsaAI BsaHI BsaXI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoNI FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MluI MscI MspA1I NarI NcoI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StyI
SwaI TaqII TatI TstI XbaI XcmI XhoI XmaI
ZraI