Restriction map for
pFN21AE4087
(Linear) (Six-base) MAPSORT of: KIEE4087.seq Check: 8297 from: 1 to: 1356
KIEE4087.orf
With 182 enzymes: SgfI *
March 22, 2012 17:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1356
Size: 718 638
AleI CACnn'nnGTG
Cuts at: 0 761 1356
Size: 761 595
AlwNI CAG_nnn'CTG
Cuts at: 0 245 377 500 795 1356
Size: 245 132 123 295 561
Fragments arranged by size:
561 295 245 132 123
ApaI G_GGCC'C
Cuts at: 0 821 1356
Size: 821 535
ApoI r'AATT_y
Cuts at: 0 3 516 911 1356
Size: 3 513 395 445
Fragments arranged by size:
513 445 395 3
AvaI C'yCGr_G
Cuts at: 0 1011 1356
Size: 1011 345
BanII G_rGCy'C
Cuts at: 0 262 809 821 1356
Size: 262 547 12 535
Fragments arranged by size:
547 535 262 12
BbvCI CC'TCA_GC
Cuts at: 0 398 1356
Size: 398 958
BglI GCCn_nnn'nGGC
Cuts at: 0 655 1356
Size: 655 701
Bme1580I G_kGCm'C
Cuts at: 0 95 821 1356
Size: 95 726 535
Fragments arranged by size:
726 535 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 1035 1356
Size: 1035 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 1356
Size: 330 1026
Bpu10I CC'TnA_GC
Cuts at: 0 256 398 1356
Size: 256 142 958
Fragments arranged by size:
958 256 142
BsaBI GATnn'nnATC
Cuts at: 0 1274 1356
Size: 1274 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 1356
Size: 259 30 1067
Fragments arranged by size:
1067 259 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 706 1313 1356
Size: 280 426 607 43
Fragments arranged by size:
607 426 280 43
BseYI C'CCAG_C
Cuts at: 0 788 1356
Size: 788 568
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 833 1356
Size: 763 70 523
Fragments arranged by size:
763 523 70
BsiHKAI G_wGCw'C
Cuts at: 0 157 262 346 362 809 999 1356
Size: 157 105 84 16 447 190 357
Fragments arranged by size:
447 357 190 157 105 84 16
BsmI GAATG_Cn'
Cuts at: 0 166 1356
Size: 166 1190
Bsp1286I G_dGCh'C
Cuts at: 0 95 157 262 346 362 809 821 999
Size: 95 62 105 84 16 447 12 178
Cuts at: 999 1356
Size: 357
Fragments arranged by size:
447 357 178 105 95 84 62 16
12
BspHI T'CATG_A
Cuts at: 0 876 1356
Size: 876 480
BstAPI GCAn_nnn'nTGC
Cuts at: 0 994 1356
Size: 994 362
BstEII G'GTnAC_C
Cuts at: 0 75 1356
Size: 75 1281
BtsI GCAGTG_nn'
Cuts at: 0 962 1356
Size: 962 394
EcoICRI GAG'CTC
Cuts at: 0 260 807 1356
Size: 260 547 549
Fragments arranged by size:
549 547 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 718 1356
Size: 330 388 638
Fragments arranged by size:
638 388 330
EcoO109I rG'GnC_Cy
Cuts at: 0 817 1356
Size: 817 539
EcoRI G'AATT_C
Cuts at: 0 3 1356
Size: 3 1353
EcoRV GAT'ATC
Cuts at: 0 897 1356
Size: 897 459
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 207 239 1356
Size: 207 32 1117
Fragments arranged by size:
1117 207 32
HincII GTy'rAC
Cuts at: 0 173 1356
Size: 173 1183
HindIII A'AGCT_T
Cuts at: 0 415 449 1356
Size: 415 34 907
Fragments arranged by size:
907 415 34
MslI CAynn'nnrTG
Cuts at: 0 52 761 947 1356
Size: 52 709 186 409
Fragments arranged by size:
709 409 186 52
NspI r_CATG'y
Cuts at: 0 786 927 1356
Size: 786 141 429
Fragments arranged by size:
786 429 141
PflMI CCAn_nnn'nTGG
Cuts at: 0 500 1356
Size: 500 856
PfoI T'CCnGG_A
Cuts at: 0 1048 1235 1356
Size: 1048 187 121
Fragments arranged by size:
1048 187 121
PspOMI G'GGCC_C
Cuts at: 0 817 1356
Size: 817 539
SacI G_AGCT'C
Cuts at: 0 262 809 1356
Size: 262 547 547
Fragments arranged by size:
547 547 262
SspI AAT'ATT
Cuts at: 0 1021 1356
Size: 1021 335
StuI AGG'CCT
Cuts at: 0 108 1332 1356
Size: 108 1224 24
Fragments arranged by size:
1224 108 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1130 1356
Size: 1130 226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 259 291 1356
Size: 259 32 1065
Fragments arranged by size:
1065 259 32
Tth111I GACn'n_nGTC
Cuts at: 0 73 377 1356
Size: 73 304 979
Fragments arranged by size:
979 304 73
XmnI GAAnn'nnTTC
Cuts at: 0 61 304 1356
Size: 61 243 1052
Fragments arranged by size:
1052 243 61
Enzymes that do cut:
AcuI AleI AlwNI ApaI ApoI AvaI BanII BbvCI
BglI Bme1580I BmrI BpmI Bpu10I BsaBI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BstAPI BstEII
BtsI EcoICRI Eco57MI EcoO109I EcoRI EcoRV Hin4I HincII
HindIII MslI NspI PflMI PfoI PspOMI SacI SspI
StuI TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoNI
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StyI
SwaI TatI XbaI XcmI XhoI XmaI ZraI