Restriction map for
pFN21AE4086
(Linear) (Six-base) MAPSORT of: KIEE4086.seq Check: 6750 from: 1 to: 1347
KIEE4086.orf
With 182 enzymes: SgfI *
July 3, 2012 18:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 1347
Size: 834 513
AflIII A'CryG_T
Cuts at: 0 680 1347
Size: 680 667
AhdI GACnn_n'nnGTC
Cuts at: 0 510 1347
Size: 510 837
AlwNI CAG_nnn'CTG
Cuts at: 0 110 597 1347
Size: 110 487 750
Fragments arranged by size:
750 487 110
ApaI G_GGCC'C
Cuts at: 0 338 1347
Size: 338 1009
BamHI G'GATC_C
Cuts at: 0 957 1347
Size: 957 390
BanII G_rGCy'C
Cuts at: 0 38 302 338 806 1347
Size: 38 264 36 468 541
Fragments arranged by size:
541 468 264 38 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 15 212 1347
Size: 15 197 1135
Fragments arranged by size:
1135 197 15
BbvCI CC'TCA_GC
Cuts at: 0 395 1347
Size: 395 952
Bme1580I G_kGCm'C
Cuts at: 0 95 338 1347
Size: 95 243 1009
Fragments arranged by size:
1009 243 95
BmgBI CAC'GTC
Cuts at: 0 720 1347
Size: 720 627
BmrI ACTGGGnnnn_n'
Cuts at: 0 1032 1347
Size: 1032 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 845 1347
Size: 715 130 502
Fragments arranged by size:
715 502 130
Bpu10I CC'TnA_GC
Cuts at: 0 395 1347
Size: 395 952
BsaBI GATnn'nnATC
Cuts at: 0 938 1347
Size: 938 409
BsaWI w'CCGG_w
Cuts at: 0 72 1347
Size: 72 1275
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1158 1188 1347
Size: 1158 30 159
Fragments arranged by size:
1158 159 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 301 586 589 1035 1158 1347
Size: 301 285 3 446 123 189
Fragments arranged by size:
446 301 285 189 123 3
BseYI C'CCAG_C
Cuts at: 0 785 1347
Size: 785 562
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 1347
Size: 183 1164
BsiHKAI G_wGCw'C
Cuts at: 0 38 144 281 302 359 806 867 874
Size: 38 106 137 21 57 447 61 7
Cuts at: 874 951 1347
Size: 77 396
Fragments arranged by size:
447 396 137 106 77 61 57 38
21 7
BsmI GAATG_Cn'
Cuts at: 0 406 1000 1347
Size: 406 594 347
Fragments arranged by size:
594 406 347
Bsp1286I G_dGCh'C
Cuts at: 0 38 95 144 281 302 338 359 806
Size: 38 57 49 137 21 36 21 447
Cuts at: 806 867 874 951 1347
Size: 61 7 77 396
Fragments arranged by size:
447 396 137 77 61 57 49 38
36 21 21 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 23 1347
Size: 23 1324
BsrGI T'GTAC_A
Cuts at: 0 683 1342 1347
Size: 683 659 5
Fragments arranged by size:
683 659 5
BstAPI GCAn_nnn'nTGC
Cuts at: 0 110 1347
Size: 110 1237
BstEII G'GTnAC_C
Cuts at: 0 75 1347
Size: 75 1272
BstXI CCAn_nnnn'nTGG
Cuts at: 0 386 1347
Size: 386 961
BstYI r'GATC_y
Cuts at: 0 69 957 1243 1347
Size: 69 888 286 104
Fragments arranged by size:
888 286 104 69
BtgI C'CryG_G
Cuts at: 0 953 1347
Size: 953 394
DrdI GACnn_nn'nnGTC
Cuts at: 0 19 1347
Size: 19 1328
EarI CTCTTCn'nnn_
Cuts at: 0 307 517 608 1314 1347
Size: 307 210 91 706 33
Fragments arranged by size:
706 307 210 91 33
EcoICRI GAG'CTC
Cuts at: 0 36 300 804 1347
Size: 36 264 504 543
Fragments arranged by size:
543 504 264 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 834 845 1347
Size: 715 119 11 502
Fragments arranged by size:
715 502 119 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 904 1140 1347
Size: 904 236 207
Fragments arranged by size:
904 236 207
EcoO109I rG'GnC_Cy
Cuts at: 0 107 335 1347
Size: 107 228 1012
Fragments arranged by size:
1012 228 107
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 204 318 1294 1347
Size: 204 114 976 53
Fragments arranged by size:
976 204 114 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 228 260 496 528 1134 1166 1228 1260
Size: 228 32 236 32 606 32 62 32
Cuts at: 1260 1347
Size: 87
Fragments arranged by size:
606 236 228 87 62 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 1073 1347
Size: 648 425 274
Fragments arranged by size:
648 425 274
MslI CAynn'nnrTG
Cuts at: 0 52 877 944 1347
Size: 52 825 67 403
Fragments arranged by size:
825 403 67 52
NspI r_CATG'y
Cuts at: 0 684 707 714 840 1347
Size: 684 23 7 126 507
Fragments arranged by size:
684 507 126 23 7
PasI CC'CwG_GG
Cuts at: 0 963 1347
Size: 963 384
PciI A'CATG_T
Cuts at: 0 680 1347
Size: 680 667
PflMI CCAn_nnn'nTGG
Cuts at: 0 969 1347
Size: 969 378
PfoI T'CCnGG_A
Cuts at: 0 731 1078 1347
Size: 731 347 269
Fragments arranged by size:
731 347 269
PsiI TTA'TAA
Cuts at: 0 418 1347
Size: 418 929
PspOMI G'GGCC_C
Cuts at: 0 334 1347
Size: 334 1013
PstI C_TGCA'G
Cuts at: 0 593 1089 1347
Size: 593 496 258
Fragments arranged by size:
593 496 258
SacI G_AGCT'C
Cuts at: 0 38 302 806 1347
Size: 38 264 504 541
Fragments arranged by size:
541 504 264 38
SapI GCTCTTCn'nnn_
Cuts at: 0 307 608 1347
Size: 307 301 739
Fragments arranged by size:
739 307 301
SfcI C'TryA_G
Cuts at: 0 589 1085 1155 1347
Size: 589 496 70 192
Fragments arranged by size:
589 496 192 70
StuI AGG'CCT
Cuts at: 0 1203 1323 1347
Size: 1203 120 24
Fragments arranged by size:
1203 120 24
TatI w'GTAC_w
Cuts at: 0 683 1342 1347
Size: 683 659 5
Fragments arranged by size:
683 659 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1158 1190 1347
Size: 1158 32 157
Fragments arranged by size:
1158 157 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1056 1347
Size: 1056 291
XmnI GAAnn'nnTTC
Cuts at: 0 61 881 1347
Size: 61 820 466
Fragments arranged by size:
820 466 61
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApaI BamHI BanII BbsI
BbvCI Bme1580I BmgBI BmrI BpmI Bpu10I BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrGI BstAPI BstEII BstXI BstYI BtgI DrdI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I MmeI MslI
NspI PasI PciI PflMI PfoI PsiI PspOMI PstI
SacI SapI SfcI StuI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmtI BplI BpuEI BsaI
BsaAI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII EaeI EagI EciI
EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StyI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI