(Linear) (Six-base) MAPSORT of: KIEE4083.seq  Check: 6882  from: 1  to: 1305



KIEE4083.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89    1305 
   Size:         89    1216
 
AflIII A'CryG_T

Cuts at:      0    1222    1305 
   Size:       1222      83
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     632     664     882     914    1305 
   Size:        632      32     218      32     391

  Fragments arranged by size: 

                 632     391     218      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     151     397    1305 
   Size:        151     246     908

  Fragments arranged by size: 

                 908     246     151
 
ApoI r'AATT_y

Cuts at:      0     427     515     775    1012    1141    1305 
   Size:        427      88     260     237     129     164

  Fragments arranged by size: 

                 427     260     237     164     129      88
 
BanI G'GyrC_C

Cuts at:      0     813    1305 
   Size:        813     492
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     454    1305 
   Size:        454     851
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     103     137    1305 
   Size:        103      34    1168

  Fragments arranged by size: 

                1168     103      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     965    1305 
   Size:        965     340
 
Bme1580I G_kGCm'C

Cuts at:      0     321    1305 
   Size:        321     984
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     186    1305 
   Size:        186    1119
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     248     270     419    1305 
   Size:        128     120      22     149     886

  Fragments arranged by size: 

                 886     149     128     120      22
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      69    1305 
   Size:         69    1236
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     290    1305 
   Size:        118     172    1015

  Fragments arranged by size: 

                1015     172     118
 
Bsp1286I G_dGCh'C

Cuts at:      0     321    1305 
   Size:        321     984
 
BspHI T'CATG_A

Cuts at:      0      48    1305 
   Size:         48    1257
 
BsrGI T'GTAC_A

Cuts at:      0     330    1305 
   Size:        330     975
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     277    1305 
   Size:        277    1028
 
BstYI r'GATC_y

Cuts at:      0     640    1305 
   Size:        640     665
 
BtgI C'CryG_G

Cuts at:      0     321    1305 
   Size:        321     984
 
BtsI GCAGTG_nn'

Cuts at:      0     165    1305 
   Size:        165    1140
 
DraI TTT'AAA

Cuts at:      0     704     837    1305 
   Size:        704     133     468

  Fragments arranged by size: 

                 704     468     133
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      33      84    1305 
   Size:         33      51    1221

  Fragments arranged by size: 

                1221      51      33
 
EarI CTCTTCn'nnn_

Cuts at:      0     445    1181    1305 
   Size:        445     736     124

  Fragments arranged by size: 

                 736     445     124
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     128     248     270     419    1305 
   Size:         89      39     120      22     149     886

  Fragments arranged by size: 

                 886     149     120      89      39      22
 
EcoO109I rG'GnC_Cy

Cuts at:      0     596    1305 
   Size:        596     709
 
EcoRI G'AATT_C

Cuts at:      0     427    1305 
   Size:        427     878
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     732    1305 
   Size:        732     573
 
MslI CAynn'nnrTG

Cuts at:      0     314     326    1305 
   Size:        314      12     979

  Fragments arranged by size: 

                 979     314      12
 
MspA1I CmG'CkG

Cuts at:      0       6    1305 
   Size:          6    1299
 
NcoI C'CATG_G

Cuts at:      0     321    1305 
   Size:        321     984
 
NspI r_CATG'y

Cuts at:      0    1226    1305 
   Size:       1226      79
 
PciI A'CATG_T

Cuts at:      0    1222    1305 
   Size:       1222      83
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     151    1075    1305 
   Size:        151     924     230

  Fragments arranged by size: 

                 924     230     151
 
PfoI T'CCnGG_A

Cuts at:      0     144    1305 
   Size:        144    1161
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1086    1118    1305 
   Size:       1086      32     187

  Fragments arranged by size: 

                1086     187      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     596    1305 
   Size:        596     709
 
PsiI TTA'TAA

Cuts at:      0     928    1305 
   Size:        928     377
 
PstI C_TGCA'G

Cuts at:      0    1237    1305 
   Size:       1237      68
 
SfcI C'TryA_G

Cuts at:      0    1233    1305 
   Size:       1233      72
 
SspI AAT'ATT

Cuts at:      0    1055    1305 
   Size:       1055     250
 
StuI AGG'CCT

Cuts at:      0      66    1305 
   Size:         66    1239
 
StyI C'CwwG_G

Cuts at:      0     321    1305 
   Size:        321     984
 
TatI w'GTAC_w

Cuts at:      0     330    1305 
   Size:        330     975
 

 Enzymes that do cut:

     AcuI   AflIII     AloI    AlwNI     ApoI     BanI     BbsI     BcgI
    BciVI Bme1580I     BplI     BpmI    BseRI     BsgI Bsp1286I    BspHI
    BsrGI   BstAPI    BstYI     BtgI     BtsI     DraI     DrdI     EarI
  Eco57MI EcoO109I    EcoRI     MmeI     MslI   MspA1I     NcoI     NspI
     PciI    PflMI     PfoI     PpiI    PpuMI     PsiI     PstI     SfcI
     SspI     StuI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI   DraIII     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI    PshAI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI