(Linear) (Six-base) MAPSORT of: KIEE4080.seq  Check: 6316  from: 1  to: 1275



KIEE4080.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:30  ..

AfeI AGC'GCT

Cuts at:      0     441    1275 
   Size:        441     834
 
AleI CACnn'nnGTG

Cuts at:      0     559    1275 
   Size:        559     716
 
AlwNI CAG_nnn'CTG

Cuts at:      0     745    1275 
   Size:        745     530
 
ApoI r'AATT_y

Cuts at:      0      95    1005    1060    1275 
   Size:         95     910      55     215

  Fragments arranged by size: 

                 910     215      95      55
 
BanI G'GyrC_C

Cuts at:      0     990    1275 
   Size:        990     285
 
BanII G_rGCy'C

Cuts at:      0     423    1129    1275 
   Size:        423     706     146

  Fragments arranged by size: 

                 706     423     146
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     324    1266    1275 
   Size:        324     942       9

  Fragments arranged by size: 

                 942     324       9
 
BclI T'GATC_A

Cuts at:      0     720    1275 
   Size:        720     555
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1023    1275 
   Size:       1023     252
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1232    1275 
   Size:       1232      43
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1143    1275 
   Size:       1143     132
 
BsaAI yAC'GTr

Cuts at:      0    1021    1275 
   Size:       1021     254
 
BsaBI GATnn'nnATC

Cuts at:      0     130    1275 
   Size:        130    1145
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     620     887    1001    1275 
   Size:        620     267     114     274

  Fragments arranged by size: 

                 620     274     267     114
 
BsiHKAI G_wGCw'C

Cuts at:      0     423    1026    1275 
   Size:        423     603     249

  Fragments arranged by size: 

                 603     423     249
 
Bsp1286I G_dGCh'C

Cuts at:      0     423    1026    1129    1275 
   Size:        423     603     103     146

  Fragments arranged by size: 

                 603     423     146     103
 
BsrDI GCAATG_nn'

Cuts at:      0    1157    1275 
   Size:       1157     118
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     585    1275 
   Size:        585     690
 
BstEII G'GTnAC_C

Cuts at:      0     103    1275 
   Size:        103    1172
 
Bsu36I CC'TnA_GG

Cuts at:      0     899    1275 
   Size:        899     376
 
BtgI C'CryG_G

Cuts at:      0     557    1275 
   Size:        557     718
 
BtsI GCAGTG_nn'

Cuts at:      0     361    1275 
   Size:        361     914
 
DraIII CAC_nnn'GTG

Cuts at:      0    1021    1275 
   Size:       1021     254
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     464    1275 
   Size:        464     811
 
EcoICRI GAG'CTC

Cuts at:      0     421    1275 
   Size:        421     854
 
EcoRI G'AATT_C

Cuts at:      0    1005    1275 
   Size:       1005     270
 
EcoRV GAT'ATC

Cuts at:      0     621    1275 
   Size:        621     654
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     674    1275 
   Size:        674     601
 
HaeII r_GCGC'y

Cuts at:      0     443    1275 
   Size:        443     832
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     314     346     945     952     977     984    1275 
   Size:        314      32     599       7      25       7     291

  Fragments arranged by size: 

                 599     314     291      32      25       7       7
 
HincII GTy'rAC

Cuts at:      0     796    1275 
   Size:        796     479
 
HindIII A'AGCT_T

Cuts at:      0     384    1275 
   Size:        384     891
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     243    1275 
   Size:        243    1032
 
MslI CAynn'nnrTG

Cuts at:      0     167     559    1275 
   Size:        167     392     716

  Fragments arranged by size: 

                 716     392     167
 
MspA1I CmG'CkG

Cuts at:      0     802    1275 
   Size:        802     473
 
NcoI C'CATG_G

Cuts at:      0     557    1275 
   Size:        557     718
 
NdeI CA'TA_TG

Cuts at:      0     584    1275 
   Size:        584     691
 
NspI r_CATG'y

Cuts at:      0     226    1275 
   Size:        226    1049
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     671     970    1275 
   Size:        671     299     305

  Fragments arranged by size: 

                 671     305     299
 
PsiI TTA'TAA

Cuts at:      0    1079    1275 
   Size:       1079     196
 
SacI G_AGCT'C

Cuts at:      0     423    1275 
   Size:        423     852
 
ScaI AGT'ACT

Cuts at:      0      55    1275 
   Size:         55    1220
 
SmlI C'TyrA_G

Cuts at:      0    1122    1275 
   Size:       1122     153
 
SphI G_CATG'C

Cuts at:      0     226    1275 
   Size:        226    1049
 
StyI C'CwwG_G

Cuts at:      0     557    1275 
   Size:        557     718
 
TatI w'GTAC_w

Cuts at:      0      53    1275 
   Size:         53    1222
 
Tth111I GACn'n_nGTC

Cuts at:      0     259    1275 
   Size:        259    1016
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1036    1275 
   Size:       1036     239
 
XmnI GAAnn'nnTTC

Cuts at:      0     455    1275 
   Size:        455     820
 

 Enzymes that do cut:

     AfeI     AleI    AlwNI     ApoI     BanI    BanII     BbsI     BclI
     BmrI     BplI    BpuEI    BsaAI    BsaBI    BseRI  BsiHKAI Bsp1286I
    BsrDI   BstAPI   BstEII   Bsu36I     BtgI     BtsI   DraIII     EciI
  EcoICRI    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII
     MmeI     MslI   MspA1I     NcoI     NdeI     NspI    PflMI     PsiI
     SacI     ScaI     SmlI     SphI     StyI     TatI  Tth111I     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI    BstBI    BstXI    BstYI  BstZ17I
    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
  Eco57MI    EcoNI EcoO109I     FseI     FspI    FspAI     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TstI     XbaI
     XhoI     XmaI     ZraI