Restriction map for
pFN21AE4078
(Linear) (Six-base) MAPSORT of: KIEE4078.seq Check: 3144 from: 1 to: 1236
KIEE4078.orf
With 182 enzymes: SgfI *
March 22, 2012 17:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1055 1236
Size: 1055 181
AatII G_ACGT'C
Cuts at: 0 1135 1236
Size: 1135 101
AccI GT'mk_AC
Cuts at: 0 1135 1236
Size: 1135 101
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 749 1236
Size: 361 388 487
Fragments arranged by size:
487 388 361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 376 1063 1205 1236
Size: 376 687 142 31
Fragments arranged by size:
687 376 142 31
AlwNI CAG_nnn'CTG
Cuts at: 0 880 1236
Size: 880 356
ApaI G_GGCC'C
Cuts at: 0 141 1236
Size: 141 1095
AvaI C'yCGr_G
Cuts at: 0 44 215 1236
Size: 44 171 1021
Fragments arranged by size:
1021 171 44
BanI G'GyrC_C
Cuts at: 0 86 203 250 442 1236
Size: 86 117 47 192 794
Fragments arranged by size:
794 192 117 86 47
BanII G_rGCy'C
Cuts at: 0 141 1004 1236
Size: 141 863 232
Fragments arranged by size:
863 232 141
BbeI G_GCGC'C
Cuts at: 0 90 207 1236
Size: 90 117 1029
Fragments arranged by size:
1029 117 90
BbsI GAAGACnn'nnnn_
Cuts at: 0 737 1236
Size: 737 499
BbvCI CC'TCA_GC
Cuts at: 0 1144 1236
Size: 1144 92
BglI GCCn_nnn'nGGC
Cuts at: 0 64 1236
Size: 64 1172
Bme1580I G_kGCm'C
Cuts at: 0 141 803 1236
Size: 141 662 433
Fragments arranged by size:
662 433 141
BmgBI CAC'GTC
Cuts at: 0 1215 1236
Size: 1215 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 268 322 963 1236
Size: 268 54 641 273
Fragments arranged by size:
641 273 268 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 639 1236
Size: 327 312 597
Fragments arranged by size:
597 327 312
Bpu10I CC'TnA_GC
Cuts at: 0 1144 1236
Size: 1144 92
BsaHI Gr'CG_yC
Cuts at: 0 87 204 1098 1132 1236
Size: 87 117 894 34 104
Fragments arranged by size:
894 117 104 87 34
BsaWI w'CCGG_w
Cuts at: 0 974 1236
Size: 974 262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 339 369 1122 1152 1236
Size: 339 30 753 30 84
Fragments arranged by size:
753 339 84 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 37 40 43 46 49 112 1012
Size: 34 3 3 3 3 3 63 900
Cuts at: 1012 1236
Size: 224
Fragments arranged by size:
900 224 63 34 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 478 699 880 1236
Size: 478 221 181 356
Fragments arranged by size:
478 356 221 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 1099 1236
Size: 554 545 137
Fragments arranged by size:
554 545 137
BsiEI CG_ry'CG
Cuts at: 0 14 68 147 1236
Size: 14 54 79 1089
Fragments arranged by size:
1089 79 54 14
BsmI GAATG_Cn'
Cuts at: 0 557 1236
Size: 557 679
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1174 1236
Size: 1174 62
Bsp1286I G_dGCh'C
Cuts at: 0 141 803 1004 1236
Size: 141 662 201 232
Fragments arranged by size:
662 232 201 141
BspEI T'CCGG_A
Cuts at: 0 974 1236
Size: 974 262
BspHI T'CATG_A
Cuts at: 0 600 1236
Size: 600 636
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1055 1236
Size: 1055 181
BsrDI GCAATG_nn'
Cuts at: 0 746 1236
Size: 746 490
BsrFI r'CCGG_y
Cuts at: 0 695 1094 1236
Size: 695 399 142
Fragments arranged by size:
695 399 142
BsrGI T'GTAC_A
Cuts at: 0 392 1236
Size: 392 844
Bsu36I CC'TnA_GG
Cuts at: 0 690 1236
Size: 690 546
BtgI C'CryG_G
Cuts at: 0 68 1236
Size: 68 1168
BtsI GCAGTG_nn'
Cuts at: 0 712 867 916 1236
Size: 712 155 49 320
Fragments arranged by size:
712 320 155 49
DraIII CAC_nnn'GTG
Cuts at: 0 507 1236
Size: 507 729
EaeI y'GGCC_r
Cuts at: 0 11 65 144 512 668 1052 1236
Size: 11 54 79 368 156 384 184
Fragments arranged by size:
384 368 184 156 79 54 11
EagI C'GGCC_G
Cuts at: 0 11 65 144 1236
Size: 11 54 79 1092
Fragments arranged by size:
1092 79 54 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 361 639 749 1236
Size: 327 34 278 110 487
Fragments arranged by size:
487 327 278 110 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 94 154 1236
Size: 94 60 1082
Fragments arranged by size:
1082 94 60
EcoO109I rG'GnC_Cy
Cuts at: 0 73 138 825 957 1106 1236
Size: 73 65 687 132 149 130
Fragments arranged by size:
687 149 132 130 73 65
HaeII r_GCGC'y
Cuts at: 0 90 180 207 321 1236
Size: 90 90 27 114 915
Fragments arranged by size:
915 114 90 90 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 339 371 1122 1154 1236
Size: 339 32 751 32 82
Fragments arranged by size:
751 339 82 32 32
HincII GTy'rAC
Cuts at: 0 1136 1236
Size: 1136 100
KasI G'GCGC_C
Cuts at: 0 86 203 1236
Size: 86 117 1033
Fragments arranged by size:
1033 117 86
MscI TGG'CCA
Cuts at: 0 514 1054 1236
Size: 514 540 182
Fragments arranged by size:
540 514 182
MslI CAynn'nnrTG
Cuts at: 0 643 1187 1236
Size: 643 544 49
Fragments arranged by size:
643 544 49
MspA1I CmG'CkG
Cuts at: 0 70 167 188 843 1128 1236
Size: 70 97 21 655 285 108
Fragments arranged by size:
655 285 108 97 70 21
NaeI GCC'GGC
Cuts at: 0 697 1236
Size: 697 539
NarI GG'CG_CC
Cuts at: 0 87 204 1236
Size: 87 117 1032
Fragments arranged by size:
1032 117 87
NgoMIV G'CCGG_C
Cuts at: 0 695 1236
Size: 695 541
NspI r_CATG'y
Cuts at: 0 135 1236
Size: 135 1101
PasI CC'CwG_GG
Cuts at: 0 874 1236
Size: 874 362
PflMI CCAn_nnn'nTGG
Cuts at: 0 677 880 1050 1218 1236
Size: 677 203 170 168 18
Fragments arranged by size:
677 203 170 168 18
PfoI T'CCnGG_A
Cuts at: 0 1217 1236
Size: 1217 19
PpuMI rG'GwC_Cy
Cuts at: 0 825 957 1236
Size: 825 132 279
Fragments arranged by size:
825 279 132
PshAI GACnn'nnGTC
Cuts at: 0 268 1097 1236
Size: 268 829 139
Fragments arranged by size:
829 268 139
PspOMI G'GGCC_C
Cuts at: 0 137 1236
Size: 137 1099
PstI C_TGCA'G
Cuts at: 0 1031 1236
Size: 1031 205
PvuII CAG'CTG
Cuts at: 0 188 843 1236
Size: 188 655 393
Fragments arranged by size:
655 393 188
SacII CC_GC'GG
Cuts at: 0 71 1236
Size: 71 1165
SalI G'TCGA_C
Cuts at: 0 1134 1236
Size: 1134 102
ScaI AGT'ACT
Cuts at: 0 592 1236
Size: 592 644
SfcI C'TryA_G
Cuts at: 0 1027 1236
Size: 1027 209
SfoI GGC'GCC
Cuts at: 0 88 205 1236
Size: 88 117 1031
Fragments arranged by size:
1031 117 88
SmaI CCC'GGG
Cuts at: 0 217 1236
Size: 217 1019
SrfI GCCC'GGGC
Cuts at: 0 217 1236
Size: 217 1019
StyI C'CwwG_G
Cuts at: 0 671 1236
Size: 671 565
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 749 1236
Size: 749 487
TatI w'GTAC_w
Cuts at: 0 392 590 1019 1157 1236
Size: 392 198 429 138 79
Fragments arranged by size:
429 392 198 138 79
Tth111I GACn'n_nGTC
Cuts at: 0 736 1236
Size: 736 500
XmaI C'CCGG_G
Cuts at: 0 215 1236
Size: 215 1021
ZraI GAC'GTC
Cuts at: 0 1133 1236
Size: 1133 103
Enzymes that do cut:
AarI AatII AccI AcuI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI Bsu36I BtgI BtsI DraIII EaeI
EagI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacII
SalI ScaI SfcI SfoI SmaI SrfI StyI TaqII
TatI Tth111I XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglII BlpI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsiHKAI BsiWI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgZI ClaI CspCI DraI DrdI EarI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SspI StuI SwaI TstI XbaI XcmI XhoI XmnI