Restriction map for
pFN21AE4075
(Linear) (Six-base) MAPSORT of: KIEE4075.seq Check: 5558 from: 1 to: 1074
KIEE4075.orf
With 182 enzymes: SgfI *
March 22, 2012 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 442 1074
Size: 442 632
AatII G_ACGT'C
Cuts at: 0 161 1074
Size: 161 913
AccI GT'mk_AC
Cuts at: 0 275 1074
Size: 275 799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 1074
Size: 197 877
AhdI GACnn_n'nnGTC
Cuts at: 0 271 1074
Size: 271 803
AleI CACnn'nnGTG
Cuts at: 0 205 1074
Size: 205 869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 553 1074
Size: 553 521
AlwNI CAG_nnn'CTG
Cuts at: 0 702 1074
Size: 702 372
AvaI C'yCGr_G
Cuts at: 0 191 707 1074
Size: 191 516 367
Fragments arranged by size:
516 367 191
BanI G'GyrC_C
Cuts at: 0 426 457 1074
Size: 426 31 617
Fragments arranged by size:
617 426 31
BanII G_rGCy'C
Cuts at: 0 199 521 1074
Size: 199 322 553
Fragments arranged by size:
553 322 199
BbsI GAAGACnn'nnnn_
Cuts at: 0 153 203 444 1074
Size: 153 50 241 630
Fragments arranged by size:
630 241 153 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 435 469 1074
Size: 435 34 605
Fragments arranged by size:
605 435 34
BclI T'GATC_A
Cuts at: 0 572 857 1074
Size: 572 285 217
Fragments arranged by size:
572 285 217
BglI GCCn_nnn'nGGC
Cuts at: 0 1068 1074
Size: 1068 6
BlpI GC'TnA_GC
Cuts at: 0 359 520 1074
Size: 359 161 554
Fragments arranged by size:
554 359 161
Bme1580I G_kGCm'C
Cuts at: 0 431 462 1074
Size: 431 31 612
Fragments arranged by size:
612 431 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 114 795 1074
Size: 114 681 279
Fragments arranged by size:
681 279 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 109 691 790 823 1074
Size: 79 30 582 99 33 251
Fragments arranged by size:
582 251 99 79 33 30
Bpu10I CC'TnA_GC
Cuts at: 0 415 1074
Size: 415 659
BsaAI yAC'GTr
Cuts at: 0 954 1074
Size: 954 120
BsaHI Gr'CG_yC
Cuts at: 0 158 1074
Size: 158 916
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 577 607 1074
Size: 577 30 467
Fragments arranged by size:
577 467 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 601 688 733 768 1024 1045 1074
Size: 394 207 87 45 35 256 21 29
Fragments arranged by size:
394 256 207 87 45 35 29 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 331 1074
Size: 261 70 743
Fragments arranged by size:
743 261 70
BsiEI CG_ry'CG
Cuts at: 0 904 1063 1074
Size: 904 159 11
Fragments arranged by size:
904 159 11
BsiHKAI G_wGCw'C
Cuts at: 0 521 953 1074
Size: 521 432 121
Fragments arranged by size:
521 432 121
Bsp1286I G_dGCh'C
Cuts at: 0 199 431 462 521 953 1074
Size: 199 232 31 59 432 121
Fragments arranged by size:
432 232 199 121 59 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 442 1074
Size: 302 140 632
Fragments arranged by size:
632 302 140
BsrBI CCG'CTC
Cuts at: 0 1059 1074
Size: 1059 15
BsrDI GCAATG_nn'
Cuts at: 0 558 1074
Size: 558 516
BstXI CCAn_nnnn'nTGG
Cuts at: 0 87 108 1074
Size: 87 21 966
Fragments arranged by size:
966 87 21
BtgI C'CryG_G
Cuts at: 0 738 809 1074
Size: 738 71 265
Fragments arranged by size:
738 265 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 516 539 550 1074
Size: 516 23 11 524
Fragments arranged by size:
524 516 23 11
BtsI GCAGTG_nn'
Cuts at: 0 484 575 671 1074
Size: 484 91 96 403
Fragments arranged by size:
484 403 96 91
EaeI y'GGCC_r
Cuts at: 0 901 956 1060 1074
Size: 901 55 104 14
Fragments arranged by size:
901 104 55 14
EagI C'GGCC_G
Cuts at: 0 901 1060 1074
Size: 901 159 14
Fragments arranged by size:
901 159 14
EarI CTCTTCn'nnn_
Cuts at: 0 386 505 809 1001 1049 1074
Size: 386 119 304 192 48 25
Fragments arranged by size:
386 304 192 119 48 25
EcoICRI GAG'CTC
Cuts at: 0 519 1074
Size: 519 555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 109 197 691 790 823 1074
Size: 79 30 88 494 99 33 251
Fragments arranged by size:
494 251 99 88 79 33 30
FspI TGC'GCA
Cuts at: 0 663 1074
Size: 663 411
HaeII r_GCGC'y
Cuts at: 0 703 832 995 1074
Size: 703 129 163 79
Fragments arranged by size:
703 163 129 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 257 289 1074
Size: 257 32 785
Fragments arranged by size:
785 257 32
MslI CAynn'nnrTG
Cuts at: 0 205 535 580 1074
Size: 205 330 45 494
Fragments arranged by size:
494 330 205 45
MspA1I CmG'CkG
Cuts at: 0 27 230 345 740 1074
Size: 27 203 115 395 334
Fragments arranged by size:
395 334 203 115 27
NcoI C'CATG_G
Cuts at: 0 809 1074
Size: 809 265
NruI TCG'CGA
Cuts at: 0 535 1074
Size: 535 539
PflMI CCAn_nnn'nTGG
Cuts at: 0 810 1074
Size: 810 264
PfoI T'CCnGG_A
Cuts at: 0 257 1074
Size: 257 817
PmlI CAC'GTG
Cuts at: 0 954 1074
Size: 954 120
PstI C_TGCA'G
Cuts at: 0 287 698 731 1074
Size: 287 411 33 343
Fragments arranged by size:
411 343 287 33
SacI G_AGCT'C
Cuts at: 0 521 1074
Size: 521 553
SacII CC_GC'GG
Cuts at: 0 741 1074
Size: 741 333
SapI GCTCTTCn'nnn_
Cuts at: 0 809 1049 1074
Size: 809 240 25
Fragments arranged by size:
809 240 25
SfcI C'TryA_G
Cuts at: 0 283 694 727 1074
Size: 283 411 33 347
Fragments arranged by size:
411 347 283 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1068 1074
Size: 1068 6
SmaI CCC'GGG
Cuts at: 0 193 709 1074
Size: 193 516 365
Fragments arranged by size:
516 365 193
SspI AAT'ATT
Cuts at: 0 405 1074
Size: 405 669
StuI AGG'CCT
Cuts at: 0 169 775 1074
Size: 169 606 299
Fragments arranged by size:
606 299 169
StyI C'CwwG_G
Cuts at: 0 353 809 848 1074
Size: 353 456 39 226
Fragments arranged by size:
456 353 226 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 577 609 1074
Size: 577 32 465
Fragments arranged by size:
577 465 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 807 882 1074
Size: 807 75 192
Fragments arranged by size:
807 192 75
XmaI C'CCGG_G
Cuts at: 0 191 707 1074
Size: 191 516 367
Fragments arranged by size:
516 367 191
ZraI GAC'GTC
Cuts at: 0 159 1074
Size: 159 915
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AleI AlfI AlwNI
AvaI BanI BanII BbsI BcgI BclI BglI BlpI
Bme1580I BplI BpmI Bpu10I BsaAI BsaHI BsaXI BseRI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstXI
BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI
FspI HaeII Hin4I MslI MspA1I NcoI NruI PflMI
PfoI PmlI PstI SacI SacII SapI SfcI SfiI
SmaI SspI StuI StyI TstI XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AloI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BciVI BfrBI BglII BmgBI BmrI BmtI
BpuEI BsaI BsaBI BsaWI BseYI BsiWI BsmI BsmBI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NsiI NspI PacI PasI PciI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SwaI TaqII TatI
Tth111I XbaI XhoI XmnI