Restriction map for
pFN21AE4073
(Linear) (Six-base) MAPSORT of: KIEE4073.seq Check: 1907 from: 1 to: 1146
KIEE4073.orf
With 182 enzymes: SgfI *
July 3, 2012 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 460 596 1146
Size: 460 136 550
Fragments arranged by size:
550 460 136
AatII G_ACGT'C
Cuts at: 0 110 1146
Size: 110 1036
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 543 1146
Size: 375 168 603
Fragments arranged by size:
603 375 168
AfeI AGC'GCT
Cuts at: 0 388 1146
Size: 388 758
AflIII A'CryG_T
Cuts at: 0 839 1146
Size: 839 307
AlwNI CAG_nnn'CTG
Cuts at: 0 1046 1146
Size: 1046 100
ApaI G_GGCC'C
Cuts at: 0 668 806 903 1146
Size: 668 138 97 243
Fragments arranged by size:
668 243 138 97
AscI GG'CGCG_CC
Cuts at: 0 740 816 1025 1146
Size: 740 76 209 121
Fragments arranged by size:
740 209 121 76
AvaI C'yCGr_G
Cuts at: 0 919 1120 1146
Size: 919 201 26
Fragments arranged by size:
919 201 26
BanI G'GyrC_C
Cuts at: 0 248 985 1115 1146
Size: 248 737 130 31
Fragments arranged by size:
737 248 130 31
BanII G_rGCy'C
Cuts at: 0 319 403 668 806 884 903 920 1146
Size: 319 84 265 138 78 19 17 226
Fragments arranged by size:
319 265 226 138 84 78 19 17
BbeI G_GCGC'C
Cuts at: 0 989 1146
Size: 989 157
BglI GCCn_nnn'nGGC
Cuts at: 0 174 1146
Size: 174 972
Bme1580I G_kGCm'C
Cuts at: 0 541 668 806 872 903 1120 1146
Size: 541 127 138 66 31 217 26
Fragments arranged by size:
541 217 138 127 66 31 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 70 138 1146
Size: 70 68 1008
Fragments arranged by size:
1008 70 68
BmtI G_CTAG'C
Cuts at: 0 647 1146
Size: 647 499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 868 1146
Size: 417 451 278
Fragments arranged by size:
451 417 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 1146
Size: 508 638
BsaI GGTCTCn'nnnn_
Cuts at: 0 29 1146
Size: 29 1117
BsaAI yAC'GTr
Cuts at: 0 840 1146
Size: 840 306
BsaBI GATnn'nnATC
Cuts at: 0 700 1146
Size: 700 446
BsaHI Gr'CG_yC
Cuts at: 0 107 986 1146
Size: 107 879 160
Fragments arranged by size:
879 160 107
BsaWI w'CCGG_w
Cuts at: 0 995 1146
Size: 995 151
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 952 1093 1096 1146
Size: 952 141 3 50
Fragments arranged by size:
952 141 50 3
BseYI C'CCAG_C
Cuts at: 0 112 156 902 1146
Size: 112 44 746 244
Fragments arranged by size:
746 244 112 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 580 1000 1146
Size: 349 231 420 146
Fragments arranged by size:
420 349 231 146
BsiEI CG_ry'CG
Cuts at: 0 178 184 468 552 764 811 1146
Size: 178 6 284 84 212 47 335
Fragments arranged by size:
335 284 212 178 84 47 6
BsiHKAI G_wGCw'C
Cuts at: 0 319 403 1146
Size: 319 84 743
Fragments arranged by size:
743 319 84
BsiWI C'GTAC_G
Cuts at: 0 836 1146
Size: 836 310
Bsp1286I G_dGCh'C
Cuts at: 0 319 403 541 668 806 872 884 903
Size: 319 84 138 127 138 66 12 19
Cuts at: 903 920 1120 1146
Size: 17 200 26
Fragments arranged by size:
319 200 138 138 127 84 66 26
19 17 12
BspEI T'CCGG_A
Cuts at: 0 995 1146
Size: 995 151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 460 596 1146
Size: 460 136 550
Fragments arranged by size:
550 460 136
BsrBI CCG'CTC
Cuts at: 0 273 1146
Size: 273 873
BsrFI r'CCGG_y
Cuts at: 0 160 265 335 503 942 1146
Size: 160 105 70 168 439 204
Fragments arranged by size:
439 204 168 160 105 70
BssHII G'CGCG_C
Cuts at: 0 740 816 1025 1146
Size: 740 76 209 121
Fragments arranged by size:
740 209 121 76
BssSI C'ACGA_G
Cuts at: 0 67 1146
Size: 67 1079
BstAPI GCAn_nnn'nTGC
Cuts at: 0 327 1046 1146
Size: 327 719 100
Fragments arranged by size:
719 327 100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 636 708 1146
Size: 636 72 438
Fragments arranged by size:
636 438 72
BstYI r'GATC_y
Cuts at: 0 1010 1146
Size: 1010 136
BtgI C'CryG_G
Cuts at: 0 178 552 707 758 764 773 797 1103
Size: 178 374 155 51 6 9 24 306
Cuts at: 1103 1128 1146
Size: 25 18
Fragments arranged by size:
374 306 178 155 51 25 24 18
9 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 728 1146
Size: 728 418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 192 227 1146
Size: 192 35 919
Fragments arranged by size:
919 192 35
DraIII CAC_nnn'GTG
Cuts at: 0 327 1020 1146
Size: 327 693 126
Fragments arranged by size:
693 327 126
EaeI y'GGCC_r
Cuts at: 0 59 175 181 465 494 549 626 761
Size: 59 116 6 284 29 55 77 135
Cuts at: 761 1146
Size: 385
Fragments arranged by size:
385 284 135 116 77 59 55 29
6
EagI C'GGCC_G
Cuts at: 0 175 181 465 549 761 1146
Size: 175 6 284 84 212 385
Fragments arranged by size:
385 284 212 175 84 6
EarI CTCTTCn'nnn_
Cuts at: 0 665 1146
Size: 665 481
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 700 1146
Size: 700 446
EcoICRI GAG'CTC
Cuts at: 0 317 401 1146
Size: 317 84 745
Fragments arranged by size:
745 317 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 417 543 868 1146
Size: 375 42 126 325 278
Fragments arranged by size:
375 325 278 126 42
EcoO109I rG'GnC_Cy
Cuts at: 0 613 664 802 803 1146
Size: 613 51 138 1 343
Fragments arranged by size:
613 343 138 51 1
HaeII r_GCGC'y
Cuts at: 0 390 510 989 1146
Size: 390 120 479 157
Fragments arranged by size:
479 390 157 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 48 80 1146
Size: 48 32 1066
Fragments arranged by size:
1066 48 32
KasI G'GCGC_C
Cuts at: 0 985 1146
Size: 985 161
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1146
Size: 428 718
MscI TGG'CCA
Cuts at: 0 61 496 1146
Size: 61 435 650
Fragments arranged by size:
650 435 61
MslI CAynn'nnrTG
Cuts at: 0 706 730 733 955 1146
Size: 706 24 3 222 191
Fragments arranged by size:
706 222 191 24 3
MspA1I CmG'CkG
Cuts at: 0 36 116 180 395 518 554 760 766
Size: 36 80 64 215 123 36 206 6
Cuts at: 766 799 897 1046 1105 1130 1146
Size: 33 98 149 59 25 16
Fragments arranged by size:
215 206 149 123 98 80 64 59
36 36 33 25 16 6
NaeI GCC'GGC
Cuts at: 0 162 944 1146
Size: 162 782 202
Fragments arranged by size:
782 202 162
NarI GG'CG_CC
Cuts at: 0 986 1146
Size: 986 160
NcoI C'CATG_G
Cuts at: 0 773 1146
Size: 773 373
NgoMIV G'CCGG_C
Cuts at: 0 160 942 1146
Size: 160 782 204
Fragments arranged by size:
782 204 160
NheI G'CTAG_C
Cuts at: 0 643 1146
Size: 643 503
NotI GC'GGCC_GC
Cuts at: 0 181 465 549 761 1146
Size: 181 284 84 212 385
Fragments arranged by size:
385 284 212 181 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 57 1020 1146
Size: 57 963 126
Fragments arranged by size:
963 126 57
PspOMI G'GGCC_C
Cuts at: 0 664 802 899 1146
Size: 664 138 97 247
Fragments arranged by size:
664 247 138 97
PstI C_TGCA'G
Cuts at: 0 1042 1146
Size: 1042 104
PvuII CAG'CTG
Cuts at: 0 116 395 1046 1146
Size: 116 279 651 100
Fragments arranged by size:
651 279 116 100
SacI G_AGCT'C
Cuts at: 0 319 403 1146
Size: 319 84 743
Fragments arranged by size:
743 319 84
SacII CC_GC'GG
Cuts at: 0 181 555 761 767 800 1106 1131 1146
Size: 181 374 206 6 33 306 25 15
Fragments arranged by size:
374 306 206 181 33 25 15 6
SexAI A'CCwGG_T
Cuts at: 0 323 1146
Size: 323 823
SfcI C'TryA_G
Cuts at: 0 1038 1146
Size: 1038 108
SfoI GGC'GCC
Cuts at: 0 987 1146
Size: 987 159
SgrAI Cr'CCGG_yG
Cuts at: 0 265 335 503 1146
Size: 265 70 168 643
Fragments arranged by size:
643 265 168 70
SmlI C'TyrA_G
Cuts at: 0 523 1146
Size: 523 623
StyI C'CwwG_G
Cuts at: 0 629 659 773 1146
Size: 629 30 114 373
Fragments arranged by size:
629 373 114 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1023 1146
Size: 1023 123
Tth111I GACn'n_nGTC
Cuts at: 0 106 1146
Size: 106 1040
XmnI GAAnn'nnTTC
Cuts at: 0 862 1146
Size: 862 284
ZraI GAC'GTC
Cuts at: 0 108 1146
Size: 108 1038
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AlwNI ApaI AscI
AvaI BanI BanII BbeI BglI Bme1580I BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI
BtgZI CspCI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI PflMI
PspOMI PstI PvuII SacI SacII SexAI SfcI SfoI
SgrAI SmlI StyI TaqII Tth111I XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BplI Bpu10I BsaXI BsmI BsmBI BspHI BsrDI
BsrGI BstBI BstEII BstZ17I Bsu36I BtsI ClaI DraI
DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NdeI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SapI SbfI ScaI SfiI SgfI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI XbaI XcmI XhoI XmaI