Restriction map for
pFN21AE4070
(Linear) (Six-base) MAPSORT of: KIEE4070.seq Check: 8553 from: 1 to: 1119
KIEE4070.orf
With 182 enzymes: SgfI *
July 3, 2012 18:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 638 838 1119
Size: 376 262 200 281
Fragments arranged by size:
376 281 262 200
AflIII A'CryG_T
Cuts at: 0 529 1106 1119
Size: 529 577 13
Fragments arranged by size:
577 529 13
AgeI A'CCGG_T
Cuts at: 0 109 893 1119
Size: 109 784 226
Fragments arranged by size:
784 226 109
ApaI G_GGCC'C
Cuts at: 0 176 1119
Size: 176 943
ApaLI G'TGCA_C
Cuts at: 0 1102 1119
Size: 1102 17
ApoI r'AATT_y
Cuts at: 0 495 824 837 1119
Size: 495 329 13 282
Fragments arranged by size:
495 329 282 13
AvaI C'yCGr_G
Cuts at: 0 733 1119
Size: 733 386
BanI G'GyrC_C
Cuts at: 0 13 1119
Size: 13 1106
BanII G_rGCy'C
Cuts at: 0 176 740 1119
Size: 176 564 379
Fragments arranged by size:
564 379 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 431 566 968 1119
Size: 431 135 402 151
Fragments arranged by size:
431 402 151 135
BglII A'GATC_T
Cuts at: 0 616 1119
Size: 616 503
Bme1580I G_kGCm'C
Cuts at: 0 176 1028 1106 1119
Size: 176 852 78 13
Fragments arranged by size:
852 176 78 13
BmgBI CAC'GTC
Cuts at: 0 20 1119
Size: 20 1099
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 641 1036 1119
Size: 89 552 395 83
Fragments arranged by size:
552 395 89 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 596 1119
Size: 596 523
BsaAI yAC'GTr
Cuts at: 0 1107 1119
Size: 1107 12
BsaWI w'CCGG_w
Cuts at: 0 109 893 1119
Size: 109 784 226
Fragments arranged by size:
784 226 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1023 1119
Size: 1023 96
BsiHKAI G_wGCw'C
Cuts at: 0 1106 1119
Size: 1106 13
Bsp1286I G_dGCh'C
Cuts at: 0 176 740 1028 1106 1119
Size: 176 564 288 78 13
Fragments arranged by size:
564 288 176 78 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 377 1045 1119
Size: 377 668 74
Fragments arranged by size:
668 377 74
BsrBI CCG'CTC
Cuts at: 0 939 1119
Size: 939 180
BsrFI r'CCGG_y
Cuts at: 0 109 627 893 1079 1119
Size: 109 518 266 186 40
Fragments arranged by size:
518 266 186 109 40
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1046 1119
Size: 1046 73
BstEII G'GTnAC_C
Cuts at: 0 1032 1119
Size: 1032 87
BstYI r'GATC_y
Cuts at: 0 435 616 1119
Size: 435 181 503
Fragments arranged by size:
503 435 181
DraIII CAC_nnn'GTG
Cuts at: 0 1046 1119
Size: 1046 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 376 638 641 838 1036 1119
Size: 89 287 262 3 197 198 83
Fragments arranged by size:
287 262 198 197 89 83 3
EcoO109I rG'GnC_Cy
Cuts at: 0 172 366 506 1119
Size: 172 194 140 613
Fragments arranged by size:
613 194 172 140
EcoRI G'AATT_C
Cuts at: 0 495 1119
Size: 495 624
HaeII r_GCGC'y
Cuts at: 0 339 1119
Size: 339 780
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 595 627 1053 1085 1119
Size: 244 32 319 32 426 32 34
Fragments arranged by size:
426 319 244 34 32 32 32
HincII GTy'rAC
Cuts at: 0 846 1119
Size: 846 273
MslI CAynn'nnrTG
Cuts at: 0 22 985 1119
Size: 22 963 134
Fragments arranged by size:
963 134 22
MspA1I CmG'CkG
Cuts at: 0 816 948 1119
Size: 816 132 171
Fragments arranged by size:
816 171 132
NaeI GCC'GGC
Cuts at: 0 629 1081 1119
Size: 629 452 38
Fragments arranged by size:
629 452 38
NgoMIV G'CCGG_C
Cuts at: 0 627 1079 1119
Size: 627 452 40
Fragments arranged by size:
627 452 40
NspI r_CATG'y
Cuts at: 0 232 1119
Size: 232 887
PmlI CAC'GTG
Cuts at: 0 1107 1119
Size: 1107 12
PpuMI rG'GwC_Cy
Cuts at: 0 366 506 1119
Size: 366 140 613
Fragments arranged by size:
613 366 140
PspOMI G'GGCC_C
Cuts at: 0 172 1119
Size: 172 947
PstI C_TGCA'G
Cuts at: 0 947 953 1119
Size: 947 6 166
Fragments arranged by size:
947 166 6
PvuII CAG'CTG
Cuts at: 0 816 948 1119
Size: 816 132 171
Fragments arranged by size:
816 171 132
ScaI AGT'ACT
Cuts at: 0 443 1119
Size: 443 676
SfcI C'TryA_G
Cuts at: 0 943 949 1119
Size: 943 6 170
Fragments arranged by size:
943 170 6
SmaI CCC'GGG
Cuts at: 0 735 1119
Size: 735 384
StyI C'CwwG_G
Cuts at: 0 743 1119
Size: 743 376
TatI w'GTAC_w
Cuts at: 0 441 1119
Size: 441 678
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 29 791 823 1119
Size: 29 762 32 296
Fragments arranged by size:
762 296 32 29
Tth111I GACn'n_nGTC
Cuts at: 0 1006 1119
Size: 1006 113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 928 1119
Size: 928 191
XmaI C'CCGG_G
Cuts at: 0 733 1119
Size: 733 386
Enzymes that do cut:
AcuI AflIII AgeI ApaI ApaLI ApoI AvaI BanI
BanII BbsI BglII Bme1580I BmgBI BpmI BsaI BsaAI
BsaWI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI
BstEII BstYI DraIII Eco57MI EcoO109I EcoRI HaeII Hin4I
HincII MslI MspA1I NaeI NgoMIV NspI PmlI PpuMI
PspOMI PstI PvuII ScaI SfcI SmaI StyI TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AlfI AloI AlwNI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmrI BmtI BplI Bpu10I BpuEI BsaBI
BsaHI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII XbaI XhoI XmnI ZraI