Restriction map for
pFN21AE4068
(Linear) (Six-base) MAPSORT of: KIEE4068.seq Check: 4964 from: 1 to: 1065
KIEE4068.orf
With 182 enzymes: SgfI *
March 22, 2012 17:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 909 1065
Size: 909 156
AatII G_ACGT'C
Cuts at: 0 511 1065
Size: 511 554
AccI GT'mk_AC
Cuts at: 0 476 1065
Size: 476 589
AfeI AGC'GCT
Cuts at: 0 1051 1065
Size: 1051 14
AflIII A'CryG_T
Cuts at: 0 539 1065
Size: 539 526
AlwNI CAG_nnn'CTG
Cuts at: 0 29 278 837 1065
Size: 29 249 559 228
Fragments arranged by size:
559 249 228 29
ApaLI G'TGCA_C
Cuts at: 0 664 1065
Size: 664 401
AvaI C'yCGr_G
Cuts at: 0 71 148 873 1065
Size: 71 77 725 192
Fragments arranged by size:
725 192 77 71
BanI G'GyrC_C
Cuts at: 0 186 418 481 1065
Size: 186 232 63 584
Fragments arranged by size:
584 232 186 63
BanII G_rGCy'C
Cuts at: 0 652 1065
Size: 652 413
BbsI GAAGACnn'nnnn_
Cuts at: 0 302 1065
Size: 302 763
BglI GCCn_nnn'nGGC
Cuts at: 0 557 1065
Size: 557 508
BlpI GC'TnA_GC
Cuts at: 0 352 1065
Size: 352 713
Bme1580I G_kGCm'C
Cuts at: 0 191 421 668 1065
Size: 191 230 247 397
Fragments arranged by size:
397 247 230 191
BmrI ACTGGGnnnn_n'
Cuts at: 0 111 173 321 1065
Size: 111 62 148 744
Fragments arranged by size:
744 148 111 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 907 962 1065
Size: 814 93 55 103
Fragments arranged by size:
814 103 93 55
Bpu10I CC'TnA_GC
Cuts at: 0 949 1065
Size: 949 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1065
Size: 898 167
BsaHI Gr'CG_yC
Cuts at: 0 9 508 1065
Size: 9 499 557
Fragments arranged by size:
557 499 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 1065
Size: 124 30 911
Fragments arranged by size:
911 124 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 589 1065
Size: 589 476
BseYI C'CCAG_C
Cuts at: 0 220 429 555 575 936 941 1015 1065
Size: 220 209 126 20 361 5 74 50
Fragments arranged by size:
361 220 209 126 74 50 20 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 238 1065
Size: 68 170 827
Fragments arranged by size:
827 170 68
BsiHKAI G_wGCw'C
Cuts at: 0 668 772 1065
Size: 668 104 293
Fragments arranged by size:
668 293 104
BsmBI CGTCTCn'nnnn_
Cuts at: 0 53 1065
Size: 53 1012
Bsp1286I G_dGCh'C
Cuts at: 0 191 421 652 668 772 1065
Size: 191 230 231 16 104 293
Fragments arranged by size:
293 231 230 191 104 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 909 1065
Size: 909 156
BsrBI CCG'CTC
Cuts at: 0 806 1065
Size: 806 259
BsrFI r'CCGG_y
Cuts at: 0 196 1065
Size: 196 869
BstAPI GCAn_nnn'nTGC
Cuts at: 0 278 959 1065
Size: 278 681 106
Fragments arranged by size:
681 278 106
BstEII G'GTnAC_C
Cuts at: 0 798 1065
Size: 798 267
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 1065
Size: 429 636
BtgI C'CryG_G
Cuts at: 0 77 773 1065
Size: 77 696 292
Fragments arranged by size:
696 292 77
DrdI GACnn_nn'nnGTC
Cuts at: 0 201 1065
Size: 201 864
EaeI y'GGCC_r
Cuts at: 0 2 407 642 865 1065
Size: 2 405 235 223 200
Fragments arranged by size:
405 235 223 200 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 996 1065
Size: 996 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 907 962 1065
Size: 814 93 55 103
Fragments arranged by size:
814 103 93 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 916 1041 1065
Size: 916 125 24
Fragments arranged by size:
916 125 24
EcoO109I rG'GnC_Cy
Cuts at: 0 26 579 945 1065
Size: 26 553 366 120
Fragments arranged by size:
553 366 120 26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 393 642 1065
Size: 393 249 423
Fragments arranged by size:
423 393 249
HaeII r_GCGC'y
Cuts at: 0 821 1053 1065
Size: 821 232 12
Fragments arranged by size:
821 232 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 453 485 1065
Size: 124 32 297 32 580
Fragments arranged by size:
580 297 124 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 491 1065
Size: 167 324 574
Fragments arranged by size:
574 324 167
MscI TGG'CCA
Cuts at: 0 4 1065
Size: 4 1061
MslI CAynn'nnrTG
Cuts at: 0 487 493 661 1065
Size: 487 6 168 404
Fragments arranged by size:
487 404 168 6
MspA1I CmG'CkG
Cuts at: 0 284 395 406 575 861 900 1065
Size: 284 111 11 169 286 39 165
Fragments arranged by size:
286 284 169 165 111 39 11
NruI TCG'CGA
Cuts at: 0 1025 1065
Size: 1025 40
PflMI CCAn_nnn'nTGG
Cuts at: 0 101 1065
Size: 101 964
PpuMI rG'GwC_Cy
Cuts at: 0 945 1065
Size: 945 120
PstI C_TGCA'G
Cuts at: 0 550 743 1065
Size: 550 193 322
Fragments arranged by size:
550 322 193
PvuII CAG'CTG
Cuts at: 0 395 575 861 1065
Size: 395 180 286 204
Fragments arranged by size:
395 286 204 180
ScaI AGT'ACT
Cuts at: 0 154 1065
Size: 154 911
SfcI C'TryA_G
Cuts at: 0 243 546 739 1065
Size: 243 303 193 326
Fragments arranged by size:
326 303 243 193
SmaI CCC'GGG
Cuts at: 0 73 1065
Size: 73 992
SmlI C'TyrA_G
Cuts at: 0 913 1065
Size: 913 152
SrfI GCCC'GGGC
Cuts at: 0 73 1065
Size: 73 992
StuI AGG'CCT
Cuts at: 0 235 1065
Size: 235 830
StyI C'CwwG_G
Cuts at: 0 422 1065
Size: 422 643
TatI w'GTAC_w
Cuts at: 0 152 389 1065
Size: 152 237 676
Fragments arranged by size:
676 237 152
Tth111I GACn'n_nGTC
Cuts at: 0 313 1065
Size: 313 752
XmaI C'CCGG_G
Cuts at: 0 71 1065
Size: 71 994
ZraI GAC'GTC
Cuts at: 0 509 1065
Size: 509 556
Enzymes that do cut:
AarI AatII AccI AfeI AflIII AlwNI ApaLI AvaI
BanI BanII BbsI BglI BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstXI
BtgI DrdI EaeI EciI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I MmeI MscI MslI MspA1I NruI PflMI
PpuMI PstI PvuII ScaI SfcI SmaI SmlI SrfI
StuI StyI TatI Tth111I XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AflII AgeI AhdI AleI AlfI
AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BsiEI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EagI EarI EcoICRI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
TaqII TstI XbaI XcmI XhoI XmnI