Restriction map for
pFN21AE4066
(Linear) (Six-base) MAPSORT of: KIEE4066.seq Check: 4569 from: 1 to: 1038
KIEE4066.orf
With 182 enzymes: SgfI *
March 22, 2012 17:18 ..
AccI GT'mk_AC
Cuts at: 0 734 1038
Size: 734 304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 611 1038
Size: 58 553 427
Fragments arranged by size:
553 427 58
AfeI AGC'GCT
Cuts at: 0 751 934 1038
Size: 751 183 104
Fragments arranged by size:
751 183 104
AflII C'TTAA_G
Cuts at: 0 366 1038
Size: 366 672
AhdI GACnn_n'nnGTC
Cuts at: 0 282 1038
Size: 282 756
AleI CACnn'nnGTG
Cuts at: 0 510 1038
Size: 510 528
AlwNI CAG_nnn'CTG
Cuts at: 0 483 651 1038
Size: 483 168 387
Fragments arranged by size:
483 387 168
ApaI G_GGCC'C
Cuts at: 0 195 349 871 1038
Size: 195 154 522 167
Fragments arranged by size:
522 195 167 154
AvaI C'yCGr_G
Cuts at: 0 71 89 143 194 264 1038
Size: 71 18 54 51 70 774
Fragments arranged by size:
774 71 70 54 51 18
BanI G'GyrC_C
Cuts at: 0 120 1038
Size: 120 918
BanII G_rGCy'C
Cuts at: 0 195 349 365 445 461 574 871 1038
Size: 195 154 16 80 16 113 297 167
Fragments arranged by size:
297 195 167 154 113 80 16 16
BbeI G_GCGC'C
Cuts at: 0 124 1038
Size: 124 914
BbvCI CC'TCA_GC
Cuts at: 0 929 1038
Size: 929 109
BglI GCCn_nnn'nGGC
Cuts at: 0 104 206 1038
Size: 104 102 832
Fragments arranged by size:
832 104 102
BglII A'GATC_T
Cuts at: 0 662 1038
Size: 662 376
BlpI GC'TnA_GC
Cuts at: 0 289 1038
Size: 289 749
Bme1580I G_kGCm'C
Cuts at: 0 195 349 871 1038
Size: 195 154 522 167
Fragments arranged by size:
522 195 167 154
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 459 1038
Size: 459 579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 1038
Size: 468 570
Bpu10I CC'TnA_GC
Cuts at: 0 929 1038
Size: 929 109
BsaHI Gr'CG_yC
Cuts at: 0 121 278 1038
Size: 121 157 760
Fragments arranged by size:
760 157 121
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 181 717 827 1038
Size: 178 3 536 110 211
Fragments arranged by size:
536 211 178 110 3
BseYI C'CCAG_C
Cuts at: 0 116 897 905 1038
Size: 116 781 8 133
Fragments arranged by size:
781 133 116 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 1038
Size: 384 654
BsiHKAI G_wGCw'C
Cuts at: 0 365 461 1038
Size: 365 96 577
Fragments arranged by size:
577 365 96
Bsp1286I G_dGCh'C
Cuts at: 0 195 349 365 445 461 574 871 1038
Size: 195 154 16 80 16 113 297 167
Fragments arranged by size:
297 195 167 154 113 80 16 16
BspHI T'CATG_A
Cuts at: 0 1019 1038
Size: 1019 19
BsrFI r'CCGG_y
Cuts at: 0 205 1038
Size: 205 833
BssHII G'CGCG_C
Cuts at: 0 269 1038
Size: 269 769
BssSI C'ACGA_G
Cuts at: 0 885 1038
Size: 885 153
BstXI CCAn_nnnn'nTGG
Cuts at: 0 512 567 1038
Size: 512 55 471
Fragments arranged by size:
512 471 55
BstYI r'GATC_y
Cuts at: 0 332 648 662 1038
Size: 332 316 14 376
Fragments arranged by size:
376 332 316 14
BtgI C'CryG_G
Cuts at: 0 187 220 1038
Size: 187 33 818
Fragments arranged by size:
818 187 33
BtsI GCAGTG_nn'
Cuts at: 0 505 517 767 778 1038
Size: 505 12 250 11 260
Fragments arranged by size:
505 260 250 12 11
EaeI y'GGCC_r
Cuts at: 0 203 973 1005 1038
Size: 203 770 32 33
Fragments arranged by size:
770 203 33 32
EarI CTCTTCn'nnn_
Cuts at: 0 583 1038
Size: 583 455
EcoICRI GAG'CTC
Cuts at: 0 363 459 1038
Size: 363 96 579
Fragments arranged by size:
579 363 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 468 611 1038
Size: 58 410 143 427
Fragments arranged by size:
427 410 143 58
EcoO109I rG'GnC_Cy
Cuts at: 0 198 345 480 780 847 868 1038
Size: 198 147 135 300 67 21 170
Fragments arranged by size:
300 198 170 147 135 67 21
HaeII r_GCGC'y
Cuts at: 0 124 753 936 1038
Size: 124 629 183 102
Fragments arranged by size:
629 183 124 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 1001 1033 1038
Size: 268 32 701 32 5
Fragments arranged by size:
701 268 32 32 5
KasI G'GCGC_C
Cuts at: 0 120 1038
Size: 120 918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 1038
Size: 161 877
MslI CAynn'nnrTG
Cuts at: 0 510 1038
Size: 510 528
MspA1I CmG'CkG
Cuts at: 0 189 222 255 308 380 425 901 969
Size: 189 33 33 53 72 45 476 68
Cuts at: 969 1038
Size: 69
Fragments arranged by size:
476 189 72 69 68 53 45 33
33
NaeI GCC'GGC
Cuts at: 0 207 1038
Size: 207 831
NarI GG'CG_CC
Cuts at: 0 121 1038
Size: 121 917
NgoMIV G'CCGG_C
Cuts at: 0 205 1038
Size: 205 833
NspI r_CATG'y
Cuts at: 0 24 678 915 1038
Size: 24 654 237 123
Fragments arranged by size:
654 237 123 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 1038
Size: 483 555
PfoI T'CCnGG_A
Cuts at: 0 445 1038
Size: 445 593
PpuMI rG'GwC_Cy
Cuts at: 0 480 780 1038
Size: 480 300 258
Fragments arranged by size:
480 300 258
PspOMI G'GGCC_C
Cuts at: 0 191 345 867 1038
Size: 191 154 522 171
Fragments arranged by size:
522 191 171 154
PstI C_TGCA'G
Cuts at: 0 512 1038
Size: 512 526
PvuII CAG'CTG
Cuts at: 0 901 969 1038
Size: 901 68 69
Fragments arranged by size:
901 69 68
SacI G_AGCT'C
Cuts at: 0 365 461 1038
Size: 365 96 577
Fragments arranged by size:
577 365 96
SacII CC_GC'GG
Cuts at: 0 190 223 1038
Size: 190 33 815
Fragments arranged by size:
815 190 33
SapI GCTCTTCn'nnn_
Cuts at: 0 583 1038
Size: 583 455
ScaI AGT'ACT
Cuts at: 0 172 1038
Size: 172 866
SexAI A'CCwGG_T
Cuts at: 0 476 1038
Size: 476 562
SfcI C'TryA_G
Cuts at: 0 508 1031 1038
Size: 508 523 7
Fragments arranged by size:
523 508 7
SfoI GGC'GCC
Cuts at: 0 122 1038
Size: 122 916
SmaI CCC'GGG
Cuts at: 0 73 91 145 196 266 1038
Size: 73 18 54 51 70 772
Fragments arranged by size:
772 73 70 54 51 18
SmlI C'TyrA_G
Cuts at: 0 4 366 1038
Size: 4 362 672
Fragments arranged by size:
672 362 4
SphI G_CATG'C
Cuts at: 0 915 1038
Size: 915 123
StuI AGG'CCT
Cuts at: 0 707 1038
Size: 707 331
StyI C'CwwG_G
Cuts at: 0 800 960 1038
Size: 800 160 78
Fragments arranged by size:
800 160 78
TatI w'GTAC_w
Cuts at: 0 170 1038
Size: 170 868
XmaI C'CCGG_G
Cuts at: 0 71 89 143 194 264 1038
Size: 71 18 54 51 70 774
Fragments arranged by size:
774 71 70 54 51 18
Enzymes that do cut:
AccI AcuI AfeI AflII AhdI AleI AlwNI ApaI
AvaI BanI BanII BbeI BbvCI BglI BglII BlpI
Bme1580I BplI BpmI Bpu10I BsaHI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BsrFI BssHII BssSI BstXI BstYI
BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII
Hin4I KasI MmeI MslI MspA1I NaeI NarI NgoMIV
NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacI
SacII SapI ScaI SexAI SfcI SfoI SmaI SmlI
SphI StuI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BcgI BciVI BclI BfrBI BmgBI BmrI BmtI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SbfI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmnI ZraI