Restriction map for
pFN21AE4062
(Linear) (Six-base) MAPSORT of: KIEE4062.seq Check: 7625 from: 1 to: 900
KIEE4062.orf
With 182 enzymes: SgfI *
July 3, 2012 18:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 735 900
Size: 735 165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 900
Size: 104 796
AleI CACnn'nnGTG
Cuts at: 0 479 900
Size: 479 421
AlwNI CAG_nnn'CTG
Cuts at: 0 586 708 750 900
Size: 586 122 42 150
Fragments arranged by size:
586 150 122 42
ApoI r'AATT_y
Cuts at: 0 94 900
Size: 94 806
BanII G_rGCy'C
Cuts at: 0 857 900
Size: 857 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 793 827 900
Size: 793 34 73
Fragments arranged by size:
793 73 34
BclI T'GATC_A
Cuts at: 0 530 900
Size: 530 370
BlpI GC'TnA_GC
Cuts at: 0 520 557 851 900
Size: 520 37 294 49
Fragments arranged by size:
520 294 49 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 843 900
Size: 843 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 900
Size: 675 225
BsaI GGTCTCn'nnnn_
Cuts at: 0 427 900
Size: 427 473
BsaAI yAC'GTr
Cuts at: 0 262 900
Size: 262 638
BsaWI w'CCGG_w
Cuts at: 0 227 900
Size: 227 673
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 27 900
Size: 27 873
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 174 900
Size: 174 726
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 208 599 610 900
Size: 49 159 391 11 290
Fragments arranged by size:
391 290 159 49 11
BsiHKAI G_wGCw'C
Cuts at: 0 425 563 857 900
Size: 425 138 294 43
Fragments arranged by size:
425 294 138 43
Bsp1286I G_dGCh'C
Cuts at: 0 425 563 857 900
Size: 425 138 294 43
Fragments arranged by size:
425 294 138 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 230 735 900
Size: 230 505 165
Fragments arranged by size:
505 230 165
BsrBI CCG'CTC
Cuts at: 0 138 900
Size: 138 762
BsrDI GCAATG_nn'
Cuts at: 0 254 900
Size: 254 646
BssSI C'ACGA_G
Cuts at: 0 430 900
Size: 430 470
BstAPI GCAn_nnn'nTGC
Cuts at: 0 735 750 900
Size: 735 15 150
Fragments arranged by size:
735 150 15
BstYI r'GATC_y
Cuts at: 0 288 332 900
Size: 288 44 568
Fragments arranged by size:
568 288 44
EaeI y'GGCC_r
Cuts at: 0 500 900
Size: 500 400
EarI CTCTTCn'nnn_
Cuts at: 0 313 409 604 900
Size: 313 96 195 296
Fragments arranged by size:
313 296 195 96
EcoICRI GAG'CTC
Cuts at: 0 855 900
Size: 855 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 675 900
Size: 104 571 225
Fragments arranged by size:
571 225 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 75 900
Size: 75 825
MscI TGG'CCA
Cuts at: 0 502 900
Size: 502 398
MslI CAynn'nnrTG
Cuts at: 0 479 496 900
Size: 479 17 404
Fragments arranged by size:
479 404 17
MspA1I CmG'CkG
Cuts at: 0 411 479 900
Size: 411 68 421
Fragments arranged by size:
421 411 68
NspI r_CATG'y
Cuts at: 0 519 726 900
Size: 519 207 174
Fragments arranged by size:
519 207 174
PasI CC'CwG_GG
Cuts at: 0 294 900
Size: 294 606
PflMI CCAn_nnn'nTGG
Cuts at: 0 414 708 900
Size: 414 294 192
Fragments arranged by size:
414 294 192
PfoI T'CCnGG_A
Cuts at: 0 539 900
Size: 539 361
PmlI CAC'GTG
Cuts at: 0 262 900
Size: 262 638
PstI C_TGCA'G
Cuts at: 0 464 694 731 755 900
Size: 464 230 37 24 145
Fragments arranged by size:
464 230 145 37 24
PvuII CAG'CTG
Cuts at: 0 411 479 900
Size: 411 68 421
Fragments arranged by size:
421 411 68
SacI G_AGCT'C
Cuts at: 0 857 900
Size: 857 43
SapI GCTCTTCn'nnn_
Cuts at: 0 409 900
Size: 409 491
SbfI CC_TGCA'GG
Cuts at: 0 694 900
Size: 694 206
SfcI C'TryA_G
Cuts at: 0 460 690 717 727 751 900
Size: 460 230 27 10 24 149
Fragments arranged by size:
460 230 149 27 24 10
SphI G_CATG'C
Cuts at: 0 726 900
Size: 726 174
StuI AGG'CCT
Cuts at: 0 178 900
Size: 178 722
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 29 900
Size: 29 871
Enzymes that do cut:
AarI AcuI AleI AlwNI ApoI BanII BcgI BclI
BlpI BplI BpmI BsaI BsaAI BsaWI BsaXI BseRI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BssSI BstAPI
BstYI EaeI EarI EcoICRI Eco57MI EcoNI MscI MslI
MspA1I NspI PasI PflMI PfoI PmlI PstI PvuII
SacI SapI SbfI SfcI SphI StuI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI
Bpu10I BpuEI BsaBI BsaHI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrFI BsrGI BssHII BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StyI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI