Restriction map for
pFN21AE4058
(Linear) (Six-base) MAPSORT of: KIEE4058.seq Check: 8690 from: 1 to: 969
KIEE4058.orf
With 182 enzymes: SgfI *
March 22, 2012 17:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 743 861 969
Size: 743 118 108
Fragments arranged by size:
743 118 108
AccI GT'mk_AC
Cuts at: 0 626 665 969
Size: 626 39 304
Fragments arranged by size:
626 304 39
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 969
Size: 781 188
AflIII A'CryG_T
Cuts at: 0 366 969
Size: 366 603
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 368 400 941 969
Size: 368 32 541 28
Fragments arranged by size:
541 368 32 28
AlwNI CAG_nnn'CTG
Cuts at: 0 681 969
Size: 681 288
ApaLI G'TGCA_C
Cuts at: 0 362 481 969
Size: 362 119 488
Fragments arranged by size:
488 362 119
ApoI r'AATT_y
Cuts at: 0 173 969
Size: 173 796
BanI G'GyrC_C
Cuts at: 0 576 969
Size: 576 393
BbsI GAAGACnn'nnnn_
Cuts at: 0 290 969
Size: 290 679
BbvCI CC'TCA_GC
Cuts at: 0 581 969
Size: 581 388
BglII A'GATC_T
Cuts at: 0 161 969
Size: 161 808
Bme1580I G_kGCm'C
Cuts at: 0 366 485 969
Size: 366 119 484
Fragments arranged by size:
484 366 119
BmgBI CAC'GTC
Cuts at: 0 369 969
Size: 369 600
BmrI ACTGGGnnnn_n'
Cuts at: 0 465 969
Size: 465 504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 969
Size: 472 497
Bpu10I CC'TnA_GC
Cuts at: 0 221 581 776 969
Size: 221 360 195 193
Fragments arranged by size:
360 221 195 193
BsaAI yAC'GTr
Cuts at: 0 330 969
Size: 330 639
BsaWI w'CCGG_w
Cuts at: 0 884 969
Size: 884 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 314 344 368 398 647 677 821 851
Size: 314 30 24 30 249 30 144 30
Cuts at: 851 941 969
Size: 90 28
Fragments arranged by size:
314 249 144 90 30 30 30 30
28 24
BseYI C'CCAG_C
Cuts at: 0 185 863 969
Size: 185 678 106
Fragments arranged by size:
678 185 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 969
Size: 594 375
BsiEI CG_ry'CG
Cuts at: 0 900 969
Size: 900 69
BsiHKAI G_wGCw'C
Cuts at: 0 366 485 969
Size: 366 119 484
Fragments arranged by size:
484 366 119
Bsp1286I G_dGCh'C
Cuts at: 0 366 485 969
Size: 366 119 484
Fragments arranged by size:
484 366 119
BspEI T'CCGG_A
Cuts at: 0 884 969
Size: 884 85
BspHI T'CATG_A
Cuts at: 0 207 969
Size: 207 762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 743 861 969
Size: 743 118 108
Fragments arranged by size:
743 118 108
BssSI C'ACGA_G
Cuts at: 0 307 969
Size: 307 662
BstYI r'GATC_y
Cuts at: 0 161 539 969
Size: 161 378 430
Fragments arranged by size:
430 378 161
BtgI C'CryG_G
Cuts at: 0 669 969
Size: 669 300
BtsI GCAGTG_nn'
Cuts at: 0 432 515 969
Size: 432 83 454
Fragments arranged by size:
454 432 83
ClaI AT'CG_AT
Cuts at: 0 195 969
Size: 195 774
DraI TTT'AAA
Cuts at: 0 30 969
Size: 30 939
DraIII CAC_nnn'GTG
Cuts at: 0 262 969
Size: 262 707
EaeI y'GGCC_r
Cuts at: 0 672 969
Size: 672 297
EarI CTCTTCn'nnn_
Cuts at: 0 152 409 508 969
Size: 152 257 99 461
Fragments arranged by size:
461 257 152 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 781 969
Size: 472 309 188
Fragments arranged by size:
472 309 188
EcoNI CCTnn'n_nnAGG
Cuts at: 0 618 969
Size: 618 351
HaeII r_GCGC'y
Cuts at: 0 231 969
Size: 231 738
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 647 679 969
Size: 647 32 290
Fragments arranged by size:
647 290 32
HincII GTy'rAC
Cuts at: 0 561 666 969
Size: 561 105 303
Fragments arranged by size:
561 303 105
HindIII A'AGCT_T
Cuts at: 0 73 969
Size: 73 896
HpaI GTT'AAC
Cuts at: 0 561 969
Size: 561 408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 969
Size: 455 514
MscI TGG'CCA
Cuts at: 0 674 969
Size: 674 295
MslI CAynn'nnrTG
Cuts at: 0 371 969
Size: 371 598
MspA1I CmG'CkG
Cuts at: 0 422 969
Size: 422 547
NcoI C'CATG_G
Cuts at: 0 669 969
Size: 669 300
NspI r_CATG'y
Cuts at: 0 317 437 908 969
Size: 317 120 471 61
Fragments arranged by size:
471 317 120 61
PacI TTA_AT'TAA
Cuts at: 0 83 969
Size: 83 886
PflMI CCAn_nnn'nTGG
Cuts at: 0 681 969
Size: 681 288
PmlI CAC'GTG
Cuts at: 0 330 969
Size: 330 639
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 368 400 941 969
Size: 368 32 541 28
Fragments arranged by size:
541 368 32 28
PstI C_TGCA'G
Cuts at: 0 219 421 705 969
Size: 219 202 284 264
Fragments arranged by size:
284 264 219 202
SalI G'TCGA_C
Cuts at: 0 664 969
Size: 664 305
SfcI C'TryA_G
Cuts at: 0 215 417 701 969
Size: 215 202 284 268
Fragments arranged by size:
284 268 215 202
SphI G_CATG'C
Cuts at: 0 317 969
Size: 317 652
StyI C'CwwG_G
Cuts at: 0 318 464 669 969
Size: 318 146 205 300
Fragments arranged by size:
318 300 205 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 886 969
Size: 886 83
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 314 346 821 853 969
Size: 314 32 475 32 116
Fragments arranged by size:
475 314 116 32 32
XbaI T'CTAG_A
Cuts at: 0 645 969
Size: 645 324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 348 471 969
Size: 348 123 498
Fragments arranged by size:
498 348 123
Enzymes that do cut:
AarI AccI AcuI AflIII AloI AlwNI ApaLI ApoI
BanI BbsI BbvCI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BsaAI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BssSI BstYI BtgI BtsI
ClaI DraI DraIII EaeI EarI Eco57MI EcoNI HaeII
Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I
NcoI NspI PacI PflMI PmlI PpiI PstI SalI
SfcI SphI StyI TaqII TstI XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BcgI BciVI BclI BfrBI BglI
BlpI BmtI BplI BpuEI BsaI BsaBI BsaHI BseRI
BsiWI BsmI BsmBI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI
DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PasI
PciI PfoI PmeI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI
Tth111I XhoI XmaI XmnI ZraI