Restriction map for
pFN21AE4057
(Linear) (Six-base) MAPSORT of: KIEE4057.seq Check: 8737 from: 1 to: 891
KIEE4057.orf
With 182 enzymes: SgfI *
July 3, 2012 18:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 696 891
Size: 696 195
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 112 307 891
Size: 112 195 584
Fragments arranged by size:
584 195 112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 322 354 505 537 866 891
Size: 322 32 151 32 329 25
Fragments arranged by size:
329 322 151 32 32 25
AlwNI CAG_nnn'CTG
Cuts at: 0 144 891
Size: 144 747
ApaI G_GGCC'C
Cuts at: 0 260 891
Size: 260 631
BanI G'GyrC_C
Cuts at: 0 840 891
Size: 840 51
BanII G_rGCy'C
Cuts at: 0 260 461 584 610 891
Size: 260 201 123 26 281
Fragments arranged by size:
281 260 201 123 26
BbvCI CC'TCA_GC
Cuts at: 0 169 311 891
Size: 169 142 580
Fragments arranged by size:
580 169 142
BglI GCCn_nnn'nGGC
Cuts at: 0 40 891
Size: 40 851
BlpI GC'TnA_GC
Cuts at: 0 604 764 891
Size: 604 160 127
Fragments arranged by size:
604 160 127
Bme1580I G_kGCm'C
Cuts at: 0 118 260 891
Size: 118 142 631
Fragments arranged by size:
631 142 118
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 800 891
Size: 800 91
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 670 853 891
Size: 346 324 183 38
Fragments arranged by size:
346 324 183 38
Bpu10I CC'TnA_GC
Cuts at: 0 169 311 891
Size: 169 142 580
Fragments arranged by size:
580 169 142
BsaI GGTCTCn'nnnn_
Cuts at: 0 208 477 891
Size: 208 269 414
Fragments arranged by size:
414 269 208
BsaBI GATnn'nnATC
Cuts at: 0 364 891
Size: 364 527
BsaHI Gr'CG_yC
Cuts at: 0 7 891
Size: 7 884
BsaWI w'CCGG_w
Cuts at: 0 377 891
Size: 377 514
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 505 535 891
Size: 505 30 356
Fragments arranged by size:
505 356 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 466 469 513 655 667 685 688
Size: 328 138 3 44 142 12 18 3
Cuts at: 688 697 776 891
Size: 9 79 115
Fragments arranged by size:
328 142 138 115 79 44 18 12
9 3 3
BseYI C'CCAG_C
Cuts at: 0 83 891
Size: 83 808
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 891
Size: 724 167
BsiEI CG_ry'CG
Cuts at: 0 16 69 891
Size: 16 53 822
Fragments arranged by size:
822 53 16
BsiHKAI G_wGCw'C
Cuts at: 0 461 584 610 637 891
Size: 461 123 26 27 254
Fragments arranged by size:
461 254 123 27 26
Bsp1286I G_dGCh'C
Cuts at: 0 118 260 461 584 610 637 891
Size: 118 142 201 123 26 27 254
Fragments arranged by size:
254 201 142 123 118 27 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 696 891
Size: 696 195
BsrFI r'CCGG_y
Cuts at: 0 15 51 891
Size: 15 36 840
Fragments arranged by size:
840 36 15
BstEII G'GTnAC_C
Cuts at: 0 402 891
Size: 402 489
BstXI CCAn_nnnn'nTGG
Cuts at: 0 877 891
Size: 877 14
Bsu36I CC'TnA_GG
Cuts at: 0 758 891
Size: 758 133
BtgI C'CryG_G
Cuts at: 0 10 891
Size: 10 881
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 34 891
Size: 34 857
BtsI GCAGTG_nn'
Cuts at: 0 448 779 891
Size: 448 331 112
Fragments arranged by size:
448 331 112
DrdI GACnn_nn'nnGTC
Cuts at: 0 400 891
Size: 400 491
EaeI y'GGCC_r
Cuts at: 0 13 66 891
Size: 13 53 825
Fragments arranged by size:
825 53 13
EagI C'GGCC_G
Cuts at: 0 13 66 891
Size: 13 53 825
Fragments arranged by size:
825 53 13
EarI CTCTTCn'nnn_
Cuts at: 0 185 380 891
Size: 185 195 511
Fragments arranged by size:
511 195 185
EcoICRI GAG'CTC
Cuts at: 0 459 582 608 891
Size: 459 123 26 283
Fragments arranged by size:
459 283 123 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 670 853 891
Size: 346 324 183 38
Fragments arranged by size:
346 324 183 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 181 891
Size: 181 710
EcoO109I rG'GnC_Cy
Cuts at: 0 221 321 411 855 891
Size: 221 100 90 444 36
Fragments arranged by size:
444 221 100 90 36
HaeII r_GCGC'y
Cuts at: 0 49 498 891
Size: 49 449 393
Fragments arranged by size:
449 393 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 184 216 801 833 891
Size: 145 32 7 32 585 32 58
Fragments arranged by size:
585 145 58 32 32 32 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 891
Size: 182 709
MslI CAynn'nnrTG
Cuts at: 0 466 846 891
Size: 466 380 45
Fragments arranged by size:
466 380 45
MspA1I CmG'CkG
Cuts at: 0 12 357 507 648 891
Size: 12 345 150 141 243
Fragments arranged by size:
345 243 150 141 12
NaeI GCC'GGC
Cuts at: 0 17 53 891
Size: 17 36 838
Fragments arranged by size:
838 36 17
NgoMIV G'CCGG_C
Cuts at: 0 15 51 891
Size: 15 36 840
Fragments arranged by size:
840 36 15
NotI GC'GGCC_GC
Cuts at: 0 66 891
Size: 66 825
NspI r_CATG'y
Cuts at: 0 427 891
Size: 427 464
PfoI T'CCnGG_A
Cuts at: 0 323 891
Size: 323 568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 505 537 891
Size: 505 32 354
Fragments arranged by size:
505 354 32
PpuMI rG'GwC_Cy
Cuts at: 0 321 411 891
Size: 321 90 480
Fragments arranged by size:
480 321 90
PspOMI G'GGCC_C
Cuts at: 0 256 891
Size: 256 635
PstI C_TGCA'G
Cuts at: 0 200 443 891
Size: 200 243 448
Fragments arranged by size:
448 243 200
PvuII CAG'CTG
Cuts at: 0 507 648 891
Size: 507 141 243
Fragments arranged by size:
507 243 141
RsrII CG'GwC_CG
Cuts at: 0 204 891
Size: 204 687
SacI G_AGCT'C
Cuts at: 0 461 584 610 891
Size: 461 123 26 281
Fragments arranged by size:
461 281 123 26
SacII CC_GC'GG
Cuts at: 0 13 891
Size: 13 878
SapI GCTCTTCn'nnn_
Cuts at: 0 185 891
Size: 185 706
SfcI C'TryA_G
Cuts at: 0 196 439 891
Size: 196 243 452
Fragments arranged by size:
452 243 196
SgrAI Cr'CCGG_yG
Cuts at: 0 51 891
Size: 51 840
StyI C'CwwG_G
Cuts at: 0 575 891
Size: 575 316
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 84 421 432 891
Size: 84 337 11 459
Fragments arranged by size:
459 337 84 11
XmnI GAAnn'nnTTC
Cuts at: 0 163 891
Size: 163 728
Enzymes that do cut:
AarI AlfI AloI AlwNI ApaI BanI BanII BbvCI
BglI BlpI Bme1580I BplI BpmI Bpu10I BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrFI BstEII BstXI Bsu36I BtgI BtgZI
BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I MmeI MslI MspA1I NaeI NgoMIV
NotI NspI PfoI PpiI PpuMI PspOMI PstI PvuII
RsrII SacI SacII SapI SfcI SgrAI StyI TaqII
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BclI BfrBI BglII BmgBI BmrI BmtI BpuEI BsaAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstYI BstZ17I ClaI CspCI
DraI DraIII EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NcoI NdeI NheI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI