Restriction map for
pFN21AE4053
(Linear) (Six-base) MAPSORT of: KIEE4053.seq Check: 6305 from: 1 to: 900
KIEE4053.orf
With 182 enzymes: SgfI *
March 22, 2012 17:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 195 900
Size: 195 705
AflIII A'CryG_T
Cuts at: 0 90 900
Size: 90 810
AlwNI CAG_nnn'CTG
Cuts at: 0 273 432 846 900
Size: 273 159 414 54
Fragments arranged by size:
414 273 159 54
BanI G'GyrC_C
Cuts at: 0 282 318 900
Size: 282 36 582
Fragments arranged by size:
582 282 36
BanII G_rGCy'C
Cuts at: 0 41 900
Size: 41 859
BbeI G_GCGC'C
Cuts at: 0 286 900
Size: 286 614
BbsI GAAGACnn'nnnn_
Cuts at: 0 80 900
Size: 80 820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 364 398 900
Size: 364 34 502
Fragments arranged by size:
502 364 34
BglI GCCn_nnn'nGGC
Cuts at: 0 235 900
Size: 235 665
BmgBI CAC'GTC
Cuts at: 0 78 93 822 900
Size: 78 15 729 78
Fragments arranged by size:
729 78 78 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 462 900
Size: 462 438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 900
Size: 523 377
Bpu10I CC'TnA_GC
Cuts at: 0 787 900
Size: 787 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 900
Size: 520 380
BsaI GGTCTCn'nnnn_
Cuts at: 0 586 900
Size: 586 314
BsaHI Gr'CG_yC
Cuts at: 0 283 623 900
Size: 283 340 277
Fragments arranged by size:
340 283 277
BsaWI w'CCGG_w
Cuts at: 0 22 152 900
Size: 22 130 748
Fragments arranged by size:
748 130 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 520 900
Size: 520 380
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 820 900
Size: 184 636 80
Fragments arranged by size:
636 184 80
BsiEI CG_ry'CG
Cuts at: 0 157 900
Size: 157 743
Bsp1286I G_dGCh'C
Cuts at: 0 41 900
Size: 41 859
BspEI T'CCGG_A
Cuts at: 0 22 900
Size: 22 878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 71 195 885 899 900
Size: 71 124 690 14 1
Fragments arranged by size:
690 124 71 14 1
BsrFI r'CCGG_y
Cuts at: 0 321 679 900
Size: 321 358 221
Fragments arranged by size:
358 321 221
BstYI r'GATC_y
Cuts at: 0 218 513 900
Size: 218 295 387
Fragments arranged by size:
387 295 218
BtgI C'CryG_G
Cuts at: 0 223 626 900
Size: 223 403 274
Fragments arranged by size:
403 274 223
BtsI GCAGTG_nn'
Cuts at: 0 759 900
Size: 759 141
DraIII CAC_nnn'GTG
Cuts at: 0 771 900
Size: 771 129
EaeI y'GGCC_r
Cuts at: 0 176 404 599 719 900
Size: 176 228 195 120 181
Fragments arranged by size:
228 195 181 176 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 790 900
Size: 790 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 900
Size: 523 377
EcoO109I rG'GnC_Cy
Cuts at: 0 105 312 843 900
Size: 105 207 531 57
Fragments arranged by size:
531 207 105 57
HaeII r_GCGC'y
Cuts at: 0 286 394 432 472 578 900
Size: 286 108 38 40 106 322
Fragments arranged by size:
322 286 108 106 40 38
HincII GTy'rAC
Cuts at: 0 606 888 900
Size: 606 282 12
Fragments arranged by size:
606 282 12
KasI G'GCGC_C
Cuts at: 0 282 900
Size: 282 618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 900
Size: 675 225
MslI CAynn'nnrTG
Cuts at: 0 820 900
Size: 820 80
MspA1I CmG'CkG
Cuts at: 0 111 120 225 345 628 834 900
Size: 111 9 105 120 283 206 66
Fragments arranged by size:
283 206 120 111 105 66 9
NarI GG'CG_CC
Cuts at: 0 283 900
Size: 283 617
PasI CC'CwG_GG
Cuts at: 0 68 900
Size: 68 832
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 846 900
Size: 771 75 54
Fragments arranged by size:
771 75 54
PfoI T'CCnGG_A
Cuts at: 0 56 900
Size: 56 844
PpuMI rG'GwC_Cy
Cuts at: 0 105 312 900
Size: 105 207 588
Fragments arranged by size:
588 207 105
PstI C_TGCA'G
Cuts at: 0 134 269 428 860 896 900
Size: 134 135 159 432 36 4
Fragments arranged by size:
432 159 135 134 36 4
PvuII CAG'CTG
Cuts at: 0 120 345 900
Size: 120 225 555
Fragments arranged by size:
555 225 120
SacII CC_GC'GG
Cuts at: 0 226 629 900
Size: 226 403 271
Fragments arranged by size:
403 271 226
SanDI GG'GwC_CC
Cuts at: 0 105 900
Size: 105 795
SbfI CC_TGCA'GG
Cuts at: 0 896 900
Size: 896 4
ScaI AGT'ACT
Cuts at: 0 727 900
Size: 727 173
SexAI A'CCwGG_T
Cuts at: 0 314 900
Size: 314 586
SfcI C'TryA_G
Cuts at: 0 130 265 424 856 892 900
Size: 130 135 159 432 36 8
Fragments arranged by size:
432 159 135 130 36 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 235 900
Size: 235 665
SfoI GGC'GCC
Cuts at: 0 284 900
Size: 284 616
SmlI C'TyrA_G
Cuts at: 0 535 900
Size: 535 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 444 900
Size: 444 456
TatI w'GTAC_w
Cuts at: 0 725 900
Size: 725 175
Tth111I GACn'n_nGTC
Cuts at: 0 706 900
Size: 706 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 900
Size: 555 345
Enzymes that do cut:
AarI AflIII AlwNI BanI BanII BbeI BbsI BcgI
BglI BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BseRI BsgI BsiEI Bsp1286I BspEI BspMI BsrFI
BstYI BtgI BtsI DraIII EaeI EciI Eco57MI EcoO109I
HaeII HincII KasI MmeI MslI MspA1I NarI PasI
PflMI PfoI PpuMI PstI PvuII SacII SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SmlI TaqII TatI
Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmtI BplI BsaAI
BsaBI BsaXI BseYI BsiHKAI BsiWI BsmI BsmBI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SapI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TstI XbaI XhoI XmaI
XmnI ZraI