(Linear) (Six-base) MAPSORT of: KIEE4052.seq  Check: 4799  from: 1  to: 696



KIEE4052.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:26  ..

AatII G_ACGT'C

Cuts at:      0      52     633     696 
   Size:         52     581      63

  Fragments arranged by size: 

                 581      63      52
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     696 
   Size:         42     654
 
AflIII A'CryG_T

Cuts at:      0      80     696 
   Size:         80     616
 
ApoI r'AATT_y

Cuts at:      0     253     696 
   Size:        253     443
 
Bme1580I G_kGCm'C

Cuts at:      0      80     585     696 
   Size:         80     505     111

  Fragments arranged by size: 

                 505     111      80
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      18     696 
   Size:         18     678
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     487     696 
   Size:        213     274     209

  Fragments arranged by size: 

                 274     213     209
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542     696 
   Size:        542     154
 
BsaAI yAC'GTr

Cuts at:      0      81     696 
   Size:         81     615
 
BsaHI Gr'CG_yC

Cuts at:      0      49      92     630     696 
   Size:         49      43     538      66

  Fragments arranged by size: 

                 538      66      49      43
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     579     609     696 
   Size:        579      30      87

  Fragments arranged by size: 

                 579      87      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     641     696 
   Size:        641      55
 
BsmI GAATG_Cn'

Cuts at:      0     127     190     696 
   Size:        127      63     506

  Fragments arranged by size: 

                 506     127      63
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     585     696 
   Size:         80     505     111

  Fragments arranged by size: 

                 505     111      80
 
BspHI T'CATG_A

Cuts at:      0     197     696 
   Size:        197     499
 
BsrFI r'CCGG_y

Cuts at:      0     523     696 
   Size:        523     173
 
Bsu36I CC'TnA_GG

Cuts at:      0     325     696 
   Size:        325     371
 
EaeI y'GGCC_r

Cuts at:      0       2     696 
   Size:          2     694
 
EarI CTCTTCn'nnn_

Cuts at:      0     268     696 
   Size:        268     428
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     213     487     696 
   Size:         42     171     274     209

  Fragments arranged by size: 

                 274     209     171      42
 
EcoRV GAT'ATC

Cuts at:      0     183     696 
   Size:        183     513
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     340     696 
   Size:        340     356
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     579     611     696 
   Size:        579      32      85

  Fragments arranged by size: 

                 579      85      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     696 
   Size:        293     403
 
MscI TGG'CCA

Cuts at:      0       4     696 
   Size:          4     692
 
MslI CAynn'nnrTG

Cuts at:      0     426     696 
   Size:        426     270
 
NaeI GCC'GGC

Cuts at:      0     525     696 
   Size:        525     171
 
NgoMIV G'CCGG_C

Cuts at:      0     523     696 
   Size:        523     173
 
PmlI CAC'GTG

Cuts at:      0      81     696 
   Size:         81     615
 
PshAI GACnn'nnGTC

Cuts at:      0     468     633     696 
   Size:        468     165      63

  Fragments arranged by size: 

                 468     165      63
 
PstI C_TGCA'G

Cuts at:      0     344     696 
   Size:        344     352
 
ScaI AGT'ACT

Cuts at:      0     226     696 
   Size:        226     470
 
SfcI C'TryA_G

Cuts at:      0     291     340     696 
   Size:        291      49     356

  Fragments arranged by size: 

                 356     291      49
 
SmlI C'TyrA_G

Cuts at:      0     557     696 
   Size:        557     139
 
TatI w'GTAC_w

Cuts at:      0      83     224     696 
   Size:         83     141     472

  Fragments arranged by size: 

                 472     141      83
 
ZraI GAC'GTC

Cuts at:      0      50     631     696 
   Size:         50     581      65

  Fragments arranged by size: 

                 581      65      50
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     ApoI Bme1580I     BmrI     BpmI    BpuEI
    BsaAI    BsaHI    BsaXI    BseRI     BsmI Bsp1286I    BspHI    BsrFI
   Bsu36I     EaeI     EarI  Eco57MI    EcoRV     FalI    Hin4I     MmeI
     MscI     MslI     NaeI   NgoMIV     PmlI    PshAI     PstI     ScaI
     SfcI     SmlI     TatI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI   MspA1I     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI