Restriction map for
pFN21AE4051
(Linear) (Six-base) MAPSORT of: KIEE4051.seq Check: 8796 from: 1 to: 882
KIEE4051.orf
With 182 enzymes: SgfI *
March 22, 2012 17:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 882
Size: 710 172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 143 882
Size: 143 739
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 365 397 882
Size: 365 32 485
Fragments arranged by size:
485 365 32
ApaI G_GGCC'C
Cuts at: 0 764 882
Size: 764 118
ApoI r'AATT_y
Cuts at: 0 354 794 882
Size: 354 440 88
Fragments arranged by size:
440 354 88
BanI G'GyrC_C
Cuts at: 0 714 882
Size: 714 168
BanII G_rGCy'C
Cuts at: 0 279 730 764 882
Size: 279 451 34 118
Fragments arranged by size:
451 279 118 34
BfrBI ATG'CAT
Cuts at: 0 120 882
Size: 120 762
BglI GCCn_nnn'nGGC
Cuts at: 0 42 500 882
Size: 42 458 382
Fragments arranged by size:
458 382 42
Bme1580I G_kGCm'C
Cuts at: 0 505 764 882
Size: 505 259 118
Fragments arranged by size:
505 259 118
BmgBI CAC'GTC
Cuts at: 0 576 882
Size: 576 306
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 259 882
Size: 259 623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 704 882
Size: 580 124 178
Fragments arranged by size:
580 178 124
Bpu10I CC'TnA_GC
Cuts at: 0 278 882
Size: 278 604
BsaI GGTCTCn'nnnn_
Cuts at: 0 116 882
Size: 116 766
BsaAI yAC'GTr
Cuts at: 0 438 882
Size: 438 444
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 266 296 365 395 560 590 830 854
Size: 266 30 69 30 165 30 240 24
Cuts at: 854 860 882
Size: 6 22
Fragments arranged by size:
266 240 165 69 30 30 30 24
22 6
BseYI C'CCAG_C
Cuts at: 0 341 737 882
Size: 341 396 145
Fragments arranged by size:
396 341 145
BsiHKAI G_wGCw'C
Cuts at: 0 279 882
Size: 279 603
BsmI GAATG_Cn'
Cuts at: 0 690 882
Size: 690 192
Bsp1286I G_dGCh'C
Cuts at: 0 279 505 730 764 882
Size: 279 226 225 34 118
Fragments arranged by size:
279 226 225 118 34
BsrBI CCG'CTC
Cuts at: 0 571 667 882
Size: 571 96 215
Fragments arranged by size:
571 215 96
BstYI r'GATC_y
Cuts at: 0 855 882
Size: 855 27
BtgI C'CryG_G
Cuts at: 0 38 135 882
Size: 38 97 747
Fragments arranged by size:
747 97 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 107 882
Size: 107 775
BtsI GCAGTG_nn'
Cuts at: 0 620 882
Size: 620 262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 497 532 882
Size: 497 35 350
Fragments arranged by size:
497 350 35
EaeI y'GGCC_r
Cuts at: 0 617 882
Size: 617 265
EarI CTCTTCn'nnn_
Cuts at: 0 629 748 882
Size: 629 119 134
Fragments arranged by size:
629 134 119
EcoICRI GAG'CTC
Cuts at: 0 277 882
Size: 277 605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 704 710 882
Size: 580 124 6 172
Fragments arranged by size:
580 172 124 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 850 882
Size: 850 32
EcoO109I rG'GnC_Cy
Cuts at: 0 760 761 882
Size: 760 1 121
Fragments arranged by size:
760 121 1
EcoRI G'AATT_C
Cuts at: 0 794 882
Size: 794 88
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 247 882
Size: 247 635
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 388 420 530 562 882
Size: 85 32 271 32 110 32 320
Fragments arranged by size:
320 271 110 85 32 32 32
HincII GTy'rAC
Cuts at: 0 450 882
Size: 450 432
HindIII A'AGCT_T
Cuts at: 0 553 882
Size: 553 329
MscI TGG'CCA
Cuts at: 0 619 882
Size: 619 263
MspA1I CmG'CkG
Cuts at: 0 40 147 335 341 882
Size: 40 107 188 6 541
Fragments arranged by size:
541 188 107 40 6
NsiI A_TGCA'T
Cuts at: 0 122 882
Size: 122 760
PmlI CAC'GTG
Cuts at: 0 438 882
Size: 438 444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 365 397 882
Size: 365 32 485
Fragments arranged by size:
485 365 32
PspOMI G'GGCC_C
Cuts at: 0 760 882
Size: 760 122
PstI C_TGCA'G
Cuts at: 0 368 882
Size: 368 514
PvuII CAG'CTG
Cuts at: 0 147 341 882
Size: 147 194 541
Fragments arranged by size:
541 194 147
SacI G_AGCT'C
Cuts at: 0 279 882
Size: 279 603
SacII CC_GC'GG
Cuts at: 0 41 882
Size: 41 841
SapI GCTCTTCn'nnn_
Cuts at: 0 629 748 882
Size: 629 119 134
Fragments arranged by size:
629 134 119
SfcI C'TryA_G
Cuts at: 0 364 882
Size: 364 518
StyI C'CwwG_G
Cuts at: 0 46 582 731 882
Size: 46 536 149 151
Fragments arranged by size:
536 151 149 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 688 882
Size: 688 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 266 298 560 592 615 647 882
Size: 266 32 262 32 23 32 235
Fragments arranged by size:
266 262 235 32 32 32 23
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 636 882
Size: 636 246
Enzymes that do cut:
AcuI AlfI AloI ApaI ApoI BanI BanII BfrBI
BglI Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI
BsaXI BseYI BsiHKAI BsmI Bsp1286I BsrBI BstYI BtgI
BtgZI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI Hin4I HincII HindIII MscI MspA1I
NsiI PmlI PpiI PspOMI PstI PvuII SacI SacII
SapI SfcI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI BmrI BmtI BpuEI BsaBI
BsaHI BsaWI BseRI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI DraI DraIII
DrdI EagI EciI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PflMI PfoI PmeI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI ZraI