Restriction map for
pFN21AE4047
(Linear) (Six-base) MAPSORT of: KIEE4047.seq Check: 2141 from: 1 to: 819
KIEE4047.orf
With 182 enzymes: SgfI *
March 22, 2012 17:15 ..
AflIII A'CryG_T
Cuts at: 0 161 819
Size: 161 658
AhdI GACnn_n'nnGTC
Cuts at: 0 411 819
Size: 411 408
AlwNI CAG_nnn'CTG
Cuts at: 0 588 759 819
Size: 588 171 60
Fragments arranged by size:
588 171 60
ApaI G_GGCC'C
Cuts at: 0 500 819
Size: 500 319
AvaI C'yCGr_G
Cuts at: 0 15 91 172 694 819
Size: 15 76 81 522 125
Fragments arranged by size:
522 125 81 76 15
BanI G'GyrC_C
Cuts at: 0 230 328 819
Size: 230 98 491
Fragments arranged by size:
491 230 98
BanII G_rGCy'C
Cuts at: 0 92 500 701 819
Size: 92 408 201 118
Fragments arranged by size:
408 201 118 92
BbsI GAAGACnn'nnnn_
Cuts at: 0 186 819
Size: 186 633
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 493 527 819
Size: 493 34 292
Fragments arranged by size:
493 292 34
BglI GCCn_nnn'nGGC
Cuts at: 0 96 105 318 484 819
Size: 96 9 213 166 335
Fragments arranged by size:
335 213 166 96 9
Bme1580I G_kGCm'C
Cuts at: 0 500 559 819
Size: 500 59 260
Fragments arranged by size:
500 260 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 198 819
Size: 198 621
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 595 819
Size: 10 585 224
Fragments arranged by size:
585 224 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 613 819
Size: 613 206
BsaHI Gr'CG_yC
Cuts at: 0 407 819
Size: 407 412
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 757 787 819
Size: 757 30 32
Fragments arranged by size:
757 32 30
BseYI C'CCAG_C
Cuts at: 0 422 819
Size: 422 397
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 169 274 355 819
Size: 104 65 105 81 464
Fragments arranged by size:
464 105 104 81 65
BsiEI CG_ry'CG
Cuts at: 0 61 100 213 819
Size: 61 39 113 606
Fragments arranged by size:
606 113 61 39
BsiHKAI G_wGCw'C
Cuts at: 0 250 366 819
Size: 250 116 453
Fragments arranged by size:
453 250 116
Bsp1286I G_dGCh'C
Cuts at: 0 92 250 366 500 559 701 819
Size: 92 158 116 134 59 142 118
Fragments arranged by size:
158 142 134 118 116 92 59
BsrBI CCG'CTC
Cuts at: 0 182 737 819
Size: 182 555 82
Fragments arranged by size:
555 182 82
BsrFI r'CCGG_y
Cuts at: 0 48 60 99 212 312 819
Size: 48 12 39 113 100 507
Fragments arranged by size:
507 113 100 48 39 12
BssSI C'ACGA_G
Cuts at: 0 532 819
Size: 532 287
BstYI r'GATC_y
Cuts at: 0 727 819
Size: 727 92
BtgI C'CryG_G
Cuts at: 0 236 261 282 353 377 819
Size: 236 25 21 71 24 442
Fragments arranged by size:
442 236 71 25 24 21
BtsI GCAGTG_nn'
Cuts at: 0 408 819
Size: 408 411
DraIII CAC_nnn'GTG
Cuts at: 0 237 378 819
Size: 237 141 441
Fragments arranged by size:
441 237 141
EaeI y'GGCC_r
Cuts at: 0 58 62 97 310 350 579 819
Size: 58 4 35 213 40 229 240
Fragments arranged by size:
240 229 213 58 40 35 4
EagI C'GGCC_G
Cuts at: 0 58 97 819
Size: 58 39 722
Fragments arranged by size:
722 58 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 225 819
Size: 225 594
EcoO109I rG'GnC_Cy
Cuts at: 0 426 496 819
Size: 426 70 323
Fragments arranged by size:
426 323 70
FseI GG_CCGG'CC
Cuts at: 0 64 819
Size: 64 755
HaeII r_GCGC'y
Cuts at: 0 55 394 759 819
Size: 55 339 365 60
Fragments arranged by size:
365 339 60 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 757 789 819
Size: 397 32 328 32 30
Fragments arranged by size:
397 328 32 32 30
MluI A'CGCG_T
Cuts at: 0 161 819
Size: 161 658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 819
Size: 101 718
MscI TGG'CCA
Cuts at: 0 352 581 819
Size: 352 229 238
Fragments arranged by size:
352 238 229
MspA1I CmG'CkG
Cuts at: 0 117 133 153 263 284 819
Size: 117 16 20 110 21 535
Fragments arranged by size:
535 117 110 21 20 16
NaeI GCC'GGC
Cuts at: 0 50 62 101 314 819
Size: 50 12 39 213 505
Fragments arranged by size:
505 213 50 39 12
NgoMIV G'CCGG_C
Cuts at: 0 48 60 99 312 819
Size: 48 12 39 213 507
Fragments arranged by size:
507 213 48 39 12
NspI r_CATG'y
Cuts at: 0 129 819
Size: 129 690
PasI CC'CwG_GG
Cuts at: 0 744 819
Size: 744 75
PflMI CCAn_nnn'nTGG
Cuts at: 0 120 588 819
Size: 120 468 231
Fragments arranged by size:
468 231 120
PpuMI rG'GwC_Cy
Cuts at: 0 426 819
Size: 426 393
PspOMI G'GGCC_C
Cuts at: 0 496 819
Size: 496 323
PstI C_TGCA'G
Cuts at: 0 764 812 819
Size: 764 48 7
Fragments arranged by size:
764 48 7
PvuII CAG'CTG
Cuts at: 0 117 153 819
Size: 117 36 666
Fragments arranged by size:
666 117 36
SacII CC_GC'GG
Cuts at: 0 264 285 819
Size: 264 21 534
Fragments arranged by size:
534 264 21
SbfI CC_TGCA'GG
Cuts at: 0 764 819
Size: 764 55
SfcI C'TryA_G
Cuts at: 0 338 760 808 819
Size: 338 422 48 11
Fragments arranged by size:
422 338 48 11
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 484 819
Size: 484 335
SmaI CCC'GGG
Cuts at: 0 93 819
Size: 93 726
SmlI C'TyrA_G
Cuts at: 0 25 610 819
Size: 25 585 209
Fragments arranged by size:
585 209 25
SrfI GCCC'GGGC
Cuts at: 0 93 819
Size: 93 726
StuI AGG'CCT
Cuts at: 0 508 819
Size: 508 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 346 819
Size: 346 473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 340 819
Size: 340 479
XmaI C'CCGG_G
Cuts at: 0 91 819
Size: 91 728
Enzymes that do cut:
AflIII AhdI AlwNI ApaI AvaI BanI BanII BbsI
BcgI BglI Bme1580I BplI BpuEI BsaI BsaHI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssSI
BstYI BtgI BtsI DraIII EaeI EagI EcoNI EcoO109I
FseI HaeII Hin4I MluI MmeI MscI MspA1I NaeI
NgoMIV NspI PasI PflMI PpuMI PspOMI PstI PvuII
SacII SbfI SfcI SfiI SmaI SmlI SrfI StuI
TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BpmI Bpu10I
BsaAI BsaBI BsaWI BseRI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MslI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SspI
StyI SwaI TatI TstI Tth111I XbaI XhoI XmnI
ZraI