Restriction map for
pFN21AE4040
(Linear) (Six-base) MAPSORT of: KIEE4040.seq Check: 1267 from: 1 to: 789
KIEE4040.orf
With 182 enzymes: SgfI *
March 22, 2012 17:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 789
Size: 751 38
AflIII A'CryG_T
Cuts at: 0 554 789
Size: 554 235
AlwNI CAG_nnn'CTG
Cuts at: 0 162 789
Size: 162 627
ApaI G_GGCC'C
Cuts at: 0 92 789
Size: 92 697
ApoI r'AATT_y
Cuts at: 0 625 685 789
Size: 625 60 104
Fragments arranged by size:
625 104 60
AvaI C'yCGr_G
Cuts at: 0 184 789
Size: 184 605
BanI G'GyrC_C
Cuts at: 0 238 789
Size: 238 551
BanII G_rGCy'C
Cuts at: 0 92 281 590 789
Size: 92 189 309 199
Fragments arranged by size:
309 199 189 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 228 262 789
Size: 228 34 527
Fragments arranged by size:
527 228 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 503 789
Size: 503 286
BglI GCCn_nnn'nGGC
Cuts at: 0 110 126 789
Size: 110 16 663
Fragments arranged by size:
663 110 16
Bme1580I G_kGCm'C
Cuts at: 0 92 131 789
Size: 92 39 658
Fragments arranged by size:
658 92 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 73 789
Size: 73 716
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 345 789
Size: 345 444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 475 687 789
Size: 184 291 212 102
Fragments arranged by size:
291 212 184 102
BsaAI yAC'GTr
Cuts at: 0 555 789
Size: 555 234
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 268 298 789
Size: 268 30 491
Fragments arranged by size:
491 268 30
BseYI C'CCAG_C
Cuts at: 0 36 94 215 789
Size: 36 58 121 574
Fragments arranged by size:
574 121 58 36
BsiEI CG_ry'CG
Cuts at: 0 135 789
Size: 135 654
BsiHKAI G_wGCw'C
Cuts at: 0 590 789
Size: 590 199
Bsp1286I G_dGCh'C
Cuts at: 0 92 131 281 590 789
Size: 92 39 150 309 199
Fragments arranged by size:
309 199 150 92 39
BsrBI CCG'CTC
Cuts at: 0 189 789
Size: 189 600
BsrDI GCAATG_nn'
Cuts at: 0 675 789
Size: 675 114
BsrGI T'GTAC_A
Cuts at: 0 557 789
Size: 557 232
BstBI TT'CG_AA
Cuts at: 0 629 789
Size: 629 160
BstYI r'GATC_y
Cuts at: 0 649 789
Size: 649 140
Bsu36I CC'TnA_GG
Cuts at: 0 267 789
Size: 267 522
BtgI C'CryG_G
Cuts at: 0 84 123 408 789
Size: 84 39 285 381
Fragments arranged by size:
381 285 84 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 40 462 789
Size: 40 422 327
Fragments arranged by size:
422 327 40
BtsI GCAGTG_nn'
Cuts at: 0 577 789
Size: 577 212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 363 398 789
Size: 363 35 391
Fragments arranged by size:
391 363 35
EaeI y'GGCC_r
Cuts at: 0 132 789
Size: 132 657
EagI C'GGCC_G
Cuts at: 0 132 789
Size: 132 657
EarI CTCTTCn'nnn_
Cuts at: 0 578 789
Size: 578 211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 394 789
Size: 19 375 395
Fragments arranged by size:
395 375 19
EcoICRI GAG'CTC
Cuts at: 0 588 789
Size: 588 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 475 687 751 789
Size: 184 291 212 64 38
Fragments arranged by size:
291 212 184 64 38
EcoO109I rG'GnC_Cy
Cuts at: 0 89 172 315 369 541 789
Size: 89 83 143 54 172 248
Fragments arranged by size:
248 172 143 89 83 54
EcoRI G'AATT_C
Cuts at: 0 685 789
Size: 685 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 763 789
Size: 268 32 463 26
Fragments arranged by size:
463 268 32 26
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 789
Size: 638 151
MslI CAynn'nnrTG
Cuts at: 0 595 789
Size: 595 194
MspA1I CmG'CkG
Cuts at: 0 15 86 125 162 215 789
Size: 15 71 39 37 53 574
Fragments arranged by size:
574 71 53 39 37 15
PfoI T'CCnGG_A
Cuts at: 0 174 789
Size: 174 615
PmlI CAC'GTG
Cuts at: 0 555 789
Size: 555 234
PpuMI rG'GwC_Cy
Cuts at: 0 172 315 369 789
Size: 172 143 54 420
Fragments arranged by size:
420 172 143 54
PspOMI G'GGCC_C
Cuts at: 0 88 789
Size: 88 701
PstI C_TGCA'G
Cuts at: 0 14 572 789
Size: 14 558 217
Fragments arranged by size:
558 217 14
PvuII CAG'CTG
Cuts at: 0 15 789
Size: 15 774
SacI G_AGCT'C
Cuts at: 0 590 789
Size: 590 199
SacII CC_GC'GG
Cuts at: 0 87 126 789
Size: 87 39 663
Fragments arranged by size:
663 87 39
SanDI GG'GwC_CC
Cuts at: 0 172 369 789
Size: 172 197 420
Fragments arranged by size:
420 197 172
SapI GCTCTTCn'nnn_
Cuts at: 0 578 789
Size: 578 211
SfcI C'TryA_G
Cuts at: 0 10 568 789
Size: 10 558 221
Fragments arranged by size:
558 221 10
TatI w'GTAC_w
Cuts at: 0 557 789
Size: 557 232
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApoI AvaI BanI BanII
BcgI BciVI BglI Bme1580I BmrI BplI BpmI BsaAI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BsrBI BsrDI BsrGI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI Hin4I
MmeI MslI MspA1I PfoI PmlI PpuMI PspOMI PstI
PvuII SacI SacII SanDI SapI SfcI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BclI BfrBI
BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BseRI BsgI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstZ17I ClaI DraI DraIII DrdI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI