Restriction map for
pFN21AE4037
(Linear) (Six-base) MAPSORT of: KIEE4037.seq Check: 1549 from: 1 to: 1563
KIEE4037.orf
With 182 enzymes: SgfI *
March 22, 2012 17:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 963 1563
Size: 963 600
AccI GT'mk_AC
Cuts at: 0 538 1563
Size: 538 1025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 705 1370 1563
Size: 497 208 665 193
Fragments arranged by size:
665 497 208 193
AflIII A'CryG_T
Cuts at: 0 1301 1563
Size: 1301 262
AlwNI CAG_nnn'CTG
Cuts at: 0 505 882 1129 1563
Size: 505 377 247 434
Fragments arranged by size:
505 434 377 247
ApaI G_GGCC'C
Cuts at: 0 699 1563
Size: 699 864
ApaLI G'TGCA_C
Cuts at: 0 154 1563
Size: 154 1409
ApoI r'AATT_y
Cuts at: 0 1270 1533 1563
Size: 1270 263 30
Fragments arranged by size:
1270 263 30
AvaI C'yCGr_G
Cuts at: 0 1246 1563
Size: 1246 317
AvrII C'CTAG_G
Cuts at: 0 690 1563
Size: 690 873
BanI G'GyrC_C
Cuts at: 0 88 595 676 1563
Size: 88 507 81 887
Fragments arranged by size:
887 507 88 81
BanII G_rGCy'C
Cuts at: 0 699 842 1240 1416 1563
Size: 699 143 398 176 147
Fragments arranged by size:
699 398 176 147 143
BbeI G_GCGC'C
Cuts at: 0 92 1563
Size: 92 1471
BbvCI CC'TCA_GC
Cuts at: 0 229 1563
Size: 229 1334
BciVI GTATCCnnnnn_n'
Cuts at: 0 942 1113 1563
Size: 942 171 450
Fragments arranged by size:
942 450 171
Bme1580I G_kGCm'C
Cuts at: 0 158 699 1563
Size: 158 541 864
Fragments arranged by size:
864 541 158
BmrI ACTGGGnnnn_n'
Cuts at: 0 988 1464 1563
Size: 988 476 99
Fragments arranged by size:
988 476 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1563
Size: 826 737
Bpu10I CC'TnA_GC
Cuts at: 0 229 743 1239 1563
Size: 229 514 496 324
Fragments arranged by size:
514 496 324 229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1164 1563
Size: 1164 399
BsaI GGTCTCn'nnnn_
Cuts at: 0 1349 1563
Size: 1349 214
BsaAI yAC'GTr
Cuts at: 0 119 1563
Size: 119 1444
BsaHI Gr'CG_yC
Cuts at: 0 89 170 1563
Size: 89 81 1393
Fragments arranged by size:
1393 89 81
BsaWI w'CCGG_w
Cuts at: 0 1214 1563
Size: 1214 349
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 814 1168 1563
Size: 814 354 395
Fragments arranged by size:
814 395 354
BseYI C'CCAG_C
Cuts at: 0 360 521 672 1115 1498 1563
Size: 360 161 151 443 383 65
Fragments arranged by size:
443 383 360 161 151 65
BsiHKAI G_wGCw'C
Cuts at: 0 158 842 1240 1416 1563
Size: 158 684 398 176 147
Fragments arranged by size:
684 398 176 158 147
BsiWI C'GTAC_G
Cuts at: 0 119 1563
Size: 119 1444
BsmI GAATG_Cn'
Cuts at: 0 258 1563
Size: 258 1305
Bsp1286I G_dGCh'C
Cuts at: 0 158 699 842 1240 1416 1563
Size: 158 541 143 398 176 147
Fragments arranged by size:
541 398 176 158 147 143
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 231 596 963 1563
Size: 231 365 367 600
Fragments arranged by size:
600 367 365 231
BsrBI CCG'CTC
Cuts at: 0 161 735 1563
Size: 161 574 828
Fragments arranged by size:
828 574 161
BsrDI GCAATG_nn'
Cuts at: 0 1463 1563
Size: 1463 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 781 1563
Size: 781 782
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 825 1563
Size: 201 624 738
Fragments arranged by size:
738 624 201
BstYI r'GATC_y
Cuts at: 0 350 1563
Size: 350 1213
Bsu36I CC'TnA_GG
Cuts at: 0 1092 1563
Size: 1092 471
BtgI C'CryG_G
Cuts at: 0 806 1563
Size: 806 757
BtsI GCAGTG_nn'
Cuts at: 0 555 1377 1563
Size: 555 822 186
Fragments arranged by size:
822 555 186
DraIII CAC_nnn'GTG
Cuts at: 0 957 1563
Size: 957 606
EaeI y'GGCC_r
Cuts at: 0 1517 1563
Size: 1517 46
EarI CTCTTCn'nnn_
Cuts at: 0 1389 1563
Size: 1389 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 811 1563
Size: 22 789 752
Fragments arranged by size:
789 752 22
EcoICRI GAG'CTC
Cuts at: 0 840 1238 1414 1563
Size: 840 398 176 149
Fragments arranged by size:
840 398 176 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 705 826 1370 1563
Size: 497 208 121 544 193
Fragments arranged by size:
544 497 208 193 121
EcoO109I rG'GnC_Cy
Cuts at: 0 518 695 1563
Size: 518 177 868
Fragments arranged by size:
868 518 177
EcoRI G'AATT_C
Cuts at: 0 1533 1563
Size: 1533 30
HaeII r_GCGC'y
Cuts at: 0 92 1563
Size: 92 1471
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1327 1359 1563
Size: 1327 32 204
Fragments arranged by size:
1327 204 32
HindIII A'AGCT_T
Cuts at: 0 1101 1563
Size: 1101 462
KasI G'GCGC_C
Cuts at: 0 88 1563
Size: 88 1475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 182 233 1563
Size: 85 97 51 1330
Fragments arranged by size:
1330 97 85 51
MscI TGG'CCA
Cuts at: 0 1519 1563
Size: 1519 44
MspA1I CmG'CkG
Cuts at: 0 228 360 1023 1115 1459 1563
Size: 228 132 663 92 344 104
Fragments arranged by size:
663 344 228 132 104 92
NarI GG'CG_CC
Cuts at: 0 89 1563
Size: 89 1474
NcoI C'CATG_G
Cuts at: 0 806 1563
Size: 806 757
NspI r_CATG'y
Cuts at: 0 1305 1563
Size: 1305 258
PasI CC'CwG_GG
Cuts at: 0 165 1563
Size: 165 1398
PciI A'CATG_T
Cuts at: 0 1301 1563
Size: 1301 262
PpuMI rG'GwC_Cy
Cuts at: 0 518 1563
Size: 518 1045
PspOMI G'GGCC_C
Cuts at: 0 695 1563
Size: 695 868
PspXI vC'TCGA_Gb
Cuts at: 0 1246 1563
Size: 1246 317
PstI C_TGCA'G
Cuts at: 0 1355 1563
Size: 1355 208
PvuII CAG'CTG
Cuts at: 0 228 360 1023 1563
Size: 228 132 663 540
Fragments arranged by size:
663 540 228 132
SacI G_AGCT'C
Cuts at: 0 842 1240 1416 1563
Size: 842 398 176 147
Fragments arranged by size:
842 398 176 147
SanDI GG'GwC_CC
Cuts at: 0 518 1563
Size: 518 1045
SapI GCTCTTCn'nnn_
Cuts at: 0 1389 1563
Size: 1389 174
SfcI C'TryA_G
Cuts at: 0 539 874 1351 1454 1563
Size: 539 335 477 103 109
Fragments arranged by size:
539 477 335 109 103
SfoI GGC'GCC
Cuts at: 0 90 1563
Size: 90 1473
SmlI C'TyrA_G
Cuts at: 0 1179 1246 1563
Size: 1179 67 317
Fragments arranged by size:
1179 317 67
StuI AGG'CCT
Cuts at: 0 73 689 1244 1563
Size: 73 616 555 319
Fragments arranged by size:
616 555 319 73
StyI C'CwwG_G
Cuts at: 0 690 806 1563
Size: 690 116 757
Fragments arranged by size:
757 690 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 618 664 1563
Size: 37 581 46 899
Fragments arranged by size:
899 581 46 37
TatI w'GTAC_w
Cuts at: 0 1070 1563
Size: 1070 493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 612 1563
Size: 612 951
XhoI C'TCGA_G
Cuts at: 0 1246 1563
Size: 1246 317
XmnI GAAnn'nnTTC
Cuts at: 0 220 1563
Size: 220 1343
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApaI ApaLI ApoI
AvaI AvrII BanI BanII BbeI BbvCI BciVI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrBI
BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII
Hin4I HindIII KasI MmeI MscI MspA1I NarI NcoI
NspI PasI PciI PpuMI PspOMI PspXI PstI PvuII
SacI SanDI SapI SfcI SfoI SmlI StuI StyI
TaqII TatI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AscI AseI AsiSI BaeI BamHI BbsI
BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BsaBI BsaXI BsgI BsiEI BsmBI BspEI BspHI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EcoNI EcoRV FalI
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XmaI ZraI