Restriction map for
pFN21AE4035
(Linear) (Six-base) MAPSORT of: KIEE4035.seq Check: 2172 from: 1 to: 738
KIEE4035.orf
With 182 enzymes: SgfI *
March 22, 2012 17:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 667 730 738
Size: 397 270 63 8
Fragments arranged by size:
397 270 63 8
AlwNI CAG_nnn'CTG
Cuts at: 0 314 738
Size: 314 424
ApaI G_GGCC'C
Cuts at: 0 489 679 738
Size: 489 190 59
Fragments arranged by size:
489 190 59
AvaI C'yCGr_G
Cuts at: 0 384 670 738
Size: 384 286 68
Fragments arranged by size:
384 286 68
BanI G'GyrC_C
Cuts at: 0 259 738
Size: 259 479
BanII G_rGCy'C
Cuts at: 0 489 679 738
Size: 489 190 59
Fragments arranged by size:
489 190 59
BglI GCCn_nnn'nGGC
Cuts at: 0 674 738
Size: 674 64
Bme1580I G_kGCm'C
Cuts at: 0 489 679 738
Size: 489 190 59
Fragments arranged by size:
489 190 59
BmrI ACTGGGnnnn_n'
Cuts at: 0 96 738
Size: 96 642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 538 738
Size: 301 237 200
Fragments arranged by size:
301 237 200
BsaI GGTCTCn'nnnn_
Cuts at: 0 535 588 738
Size: 535 53 150
Fragments arranged by size:
535 150 53
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 738
Size: 130 608
BseYI C'CCAG_C
Cuts at: 0 255 680 738
Size: 255 425 58
Fragments arranged by size:
425 255 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 738
Size: 228 510
BsmI GAATG_Cn'
Cuts at: 0 684 738
Size: 684 54
Bsp1286I G_dGCh'C
Cuts at: 0 489 679 738
Size: 489 190 59
Fragments arranged by size:
489 190 59
BsrDI GCAATG_nn'
Cuts at: 0 533 738
Size: 533 205
BsrFI r'CCGG_y
Cuts at: 0 134 247 738
Size: 134 113 491
Fragments arranged by size:
491 134 113
BstXI CCAn_nnnn'nTGG
Cuts at: 0 75 738
Size: 75 663
BstYI r'GATC_y
Cuts at: 0 194 650 738
Size: 194 456 88
Fragments arranged by size:
456 194 88
BtgI C'CryG_G
Cuts at: 0 391 524 738
Size: 391 133 214
Fragments arranged by size:
391 214 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 531 738
Size: 531 207
BtsI GCAGTG_nn'
Cuts at: 0 347 597 738
Size: 347 250 141
Fragments arranged by size:
347 250 141
DraIII CAC_nnn'GTG
Cuts at: 0 175 371 738
Size: 175 196 367
Fragments arranged by size:
367 196 175
EaeI y'GGCC_r
Cuts at: 0 132 452 738
Size: 132 320 286
Fragments arranged by size:
320 286 132
EarI CTCTTCn'nnn_
Cuts at: 0 559 738
Size: 559 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 676 738
Size: 676 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 397 538 667 730 738
Size: 301 96 141 129 63 8
Fragments arranged by size:
301 141 129 96 63 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 712 738
Size: 712 26
EcoO109I rG'GnC_Cy
Cuts at: 0 381 485 675 676 738
Size: 381 104 190 1 62
Fragments arranged by size:
381 190 104 62 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 165 197 738
Size: 165 32 541
Fragments arranged by size:
541 165 32
MspA1I CmG'CkG
Cuts at: 0 393 450 516 606 700 738
Size: 393 57 66 90 94 38
Fragments arranged by size:
393 94 90 66 57 38
NaeI GCC'GGC
Cuts at: 0 136 738
Size: 136 602
NgoMIV G'CCGG_C
Cuts at: 0 134 738
Size: 134 604
PflMI CCAn_nnn'nTGG
Cuts at: 0 108 738
Size: 108 630
PfoI T'CCnGG_A
Cuts at: 0 653 738
Size: 653 85
PpuMI rG'GwC_Cy
Cuts at: 0 381 738
Size: 381 357
PshAI GACnn'nnGTC
Cuts at: 0 580 738
Size: 580 158
PspOMI G'GGCC_C
Cuts at: 0 485 675 738
Size: 485 190 63
Fragments arranged by size:
485 190 63
PvuII CAG'CTG
Cuts at: 0 516 606 700 738
Size: 516 90 94 38
Fragments arranged by size:
516 94 90 38
RsrII CG'GwC_CG
Cuts at: 0 94 738
Size: 94 644
SacII CC_GC'GG
Cuts at: 0 394 738
Size: 394 344
SmaI CCC'GGG
Cuts at: 0 386 738
Size: 386 352
StyI C'CwwG_G
Cuts at: 0 68 161 738
Size: 68 93 577
Fragments arranged by size:
577 93 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 247 738
Size: 247 491
TatI w'GTAC_w
Cuts at: 0 608 738
Size: 608 130
XmaI C'CCGG_G
Cuts at: 0 384 738
Size: 384 354
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanI BanII BglI Bme1580I
BmrI BpmI BsaI BseRI BseYI BsgI BsmI Bsp1286I
BsrDI BsrFI BstXI BstYI BtgI BtgZI BtsI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I Hin4I MspA1I
NaeI NgoMIV PflMI PfoI PpuMI PshAI PspOMI PvuII
RsrII SacII SmaI StyI TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI
PstI PvuI SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI