Restriction map for
pFN21AE4033
(Linear) (Six-base) MAPSORT of: KIEE4033.seq Check: 5478 from: 1 to: 726
KIEE4033.orf
With 182 enzymes: SgfI *
March 22, 2012 17:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 456 726
Size: 456 270
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 726
Size: 688 38
AlwNI CAG_nnn'CTG
Cuts at: 0 666 690 726
Size: 666 24 36
Fragments arranged by size:
666 36 24
ApoI r'AATT_y
Cuts at: 0 524 726
Size: 524 202
AvaI C'yCGr_G
Cuts at: 0 46 247 726
Size: 46 201 479
Fragments arranged by size:
479 201 46
BanI G'GyrC_C
Cuts at: 0 100 726
Size: 100 626
BbeI G_GCGC'C
Cuts at: 0 104 726
Size: 104 622
BciVI GTATCCnnnnn_n'
Cuts at: 0 234 726
Size: 234 492
BglI GCCn_nnn'nGGC
Cuts at: 0 113 726
Size: 113 613
BlpI GC'TnA_GC
Cuts at: 0 10 726
Size: 10 716
Bme1580I G_kGCm'C
Cuts at: 0 26 726
Size: 26 700
BmtI G_CTAG'C
Cuts at: 0 496 726
Size: 496 230
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 72 726
Size: 72 654
BsaHI Gr'CG_yC
Cuts at: 0 101 254 726
Size: 101 153 472
Fragments arranged by size:
472 153 101
BsaWI w'CCGG_w
Cuts at: 0 392 726
Size: 392 334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 208 238 726
Size: 208 30 488
Fragments arranged by size:
488 208 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 45 726
Size: 45 681
BseYI C'CCAG_C
Cuts at: 0 96 504 726
Size: 96 408 222
Fragments arranged by size:
408 222 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 490 701 726
Size: 328 162 211 25
Fragments arranged by size:
328 211 162 25
BsiEI CG_ry'CG
Cuts at: 0 7 108 726
Size: 7 101 618
Fragments arranged by size:
618 101 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 666 726
Size: 666 60
Bsp1286I G_dGCh'C
Cuts at: 0 26 726
Size: 26 700
BspEI T'CCGG_A
Cuts at: 0 392 726
Size: 392 334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 456 726
Size: 456 270
BsrBI CCG'CTC
Cuts at: 0 9 647 726
Size: 9 638 79
Fragments arranged by size:
638 79 9
BsrDI GCAATG_nn'
Cuts at: 0 131 726
Size: 131 595
BsrFI r'CCGG_y
Cuts at: 0 103 726
Size: 103 623
BstAPI GCAn_nnn'nTGC
Cuts at: 0 30 726
Size: 30 696
BstXI CCAn_nnnn'nTGG
Cuts at: 0 504 726
Size: 504 222
BstYI r'GATC_y
Cuts at: 0 233 260 726
Size: 233 27 466
Fragments arranged by size:
466 233 27
BtsI GCAGTG_nn'
Cuts at: 0 316 726
Size: 316 410
EaeI y'GGCC_r
Cuts at: 0 4 105 726
Size: 4 101 621
Fragments arranged by size:
621 101 4
EagI C'GGCC_G
Cuts at: 0 4 105 726
Size: 4 101 621
Fragments arranged by size:
621 101 4
EarI CTCTTCn'nnn_
Cuts at: 0 615 664 726
Size: 615 49 62
Fragments arranged by size:
615 62 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 726
Size: 688 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 277 726
Size: 277 449
EcoO109I rG'GnC_Cy
Cuts at: 0 447 663 726
Size: 447 216 63
Fragments arranged by size:
447 216 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 693 726
Size: 693 33
HaeII r_GCGC'y
Cuts at: 0 104 726
Size: 104 622
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 280 312 614 646 726
Size: 208 32 40 32 302 32 80
Fragments arranged by size:
302 208 80 40 32 32 32
KasI G'GCGC_C
Cuts at: 0 100 726
Size: 100 626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 726
Size: 396 330
MslI CAynn'nnrTG
Cuts at: 0 430 726
Size: 430 296
NaeI GCC'GGC
Cuts at: 0 105 726
Size: 105 621
NarI GG'CG_CC
Cuts at: 0 101 726
Size: 101 625
NgoMIV G'CCGG_C
Cuts at: 0 103 726
Size: 103 623
NheI G'CTAG_C
Cuts at: 0 492 726
Size: 492 234
NotI GC'GGCC_GC
Cuts at: 0 4 726
Size: 4 722
NspI r_CATG'y
Cuts at: 0 18 460 660 726
Size: 18 442 200 66
Fragments arranged by size:
442 200 66 18
PfoI T'CCnGG_A
Cuts at: 0 236 726
Size: 236 490
PpuMI rG'GwC_Cy
Cuts at: 0 447 663 726
Size: 447 216 63
Fragments arranged by size:
447 216 63
PstI C_TGCA'G
Cuts at: 0 233 726
Size: 233 493
SapI GCTCTTCn'nnn_
Cuts at: 0 615 726
Size: 615 111
SfcI C'TryA_G
Cuts at: 0 229 726
Size: 229 497
SfoI GGC'GCC
Cuts at: 0 102 726
Size: 102 624
SmaI CCC'GGG
Cuts at: 0 249 726
Size: 249 477
SphI G_CATG'C
Cuts at: 0 460 726
Size: 460 266
StyI C'CwwG_G
Cuts at: 0 74 726
Size: 74 652
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 54 726
Size: 54 672
XmaI C'CCGG_G
Cuts at: 0 247 726
Size: 247 479
Enzymes that do cut:
AarI AcuI AlwNI ApoI AvaI BanI BbeI BciVI
BglI BlpI Bme1580I BmtI BplI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtsI EaeI
EagI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
KasI MmeI MslI NaeI NarI NgoMIV NheI NotI
NspI PfoI PpuMI PstI SapI SfcI SfoI SmaI
SphI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbsI BbvCI BcgI
BclI BfrBI BglII BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsiHKAI BsiWI BsmI BspHI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MscI MspA1I NcoI NdeI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI