Restriction map for
pFN21AE4029
(Linear) (Six-base) MAPSORT of: KIEE4029.seq Check: 8439 from: 1 to: 708
KIEE4029.orf
With 182 enzymes: SgfI *
March 22, 2012 17:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 163 708
Size: 163 545
Acc65I G'GTAC_C
Cuts at: 0 164 708
Size: 164 544
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 708
Size: 312 396
AlwNI CAG_nnn'CTG
Cuts at: 0 519 708
Size: 519 189
ApaI G_GGCC'C
Cuts at: 0 48 493 708
Size: 48 445 215
Fragments arranged by size:
445 215 48
AvaI C'yCGr_G
Cuts at: 0 379 708
Size: 379 329
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 296 329 708
Size: 296 33 379
Fragments arranged by size:
379 296 33
BanI G'GyrC_C
Cuts at: 0 118 164 405 424 708
Size: 118 46 241 19 284
Fragments arranged by size:
284 241 118 46 19
BanII G_rGCy'C
Cuts at: 0 48 231 248 493 708
Size: 48 183 17 245 215
Fragments arranged by size:
245 215 183 48 17
BbeI G_GCGC'C
Cuts at: 0 122 409 708
Size: 122 287 299
Fragments arranged by size:
299 287 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 306 708
Size: 306 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 123 157 524 558 708
Size: 123 34 367 34 150
Fragments arranged by size:
367 150 123 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 297 708
Size: 297 411
BglI GCCn_nnn'nGGC
Cuts at: 0 488 708
Size: 488 220
BlpI GC'TnA_GC
Cuts at: 0 16 558 708
Size: 16 542 150
Fragments arranged by size:
542 150 16
Bme1580I G_kGCm'C
Cuts at: 0 48 427 493 500 708
Size: 48 379 66 7 208
Fragments arranged by size:
379 208 66 48 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 708
Size: 655 53
Bpu10I CC'TnA_GC
Cuts at: 0 512 708
Size: 512 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 708
Size: 571 137
BsaHI Gr'CG_yC
Cuts at: 0 119 406 708
Size: 119 287 302
Fragments arranged by size:
302 287 119
BsaWI w'CCGG_w
Cuts at: 0 231 708
Size: 231 477
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 218 248 708
Size: 218 30 460
Fragments arranged by size:
460 218 30
BseYI C'CCAG_C
Cuts at: 0 87 343 708
Size: 87 256 365
Fragments arranged by size:
365 256 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 708
Size: 358 350
BsiHKAI G_wGCw'C
Cuts at: 0 231 248 559 623 708
Size: 231 17 311 64 85
Fragments arranged by size:
311 231 85 64 17
BsmI GAATG_Cn'
Cuts at: 0 654 708
Size: 654 54
Bsp1286I G_dGCh'C
Cuts at: 0 48 231 248 427 493 500 559 623
Size: 48 183 17 179 66 7 59 64
Cuts at: 623 708
Size: 85
Fragments arranged by size:
183 179 85 66 64 59 48 17
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 163 708
Size: 163 545
BsrBI CCG'CTC
Cuts at: 0 39 708
Size: 39 669
BsrDI GCAATG_nn'
Cuts at: 0 165 708
Size: 165 543
BsrFI r'CCGG_y
Cuts at: 0 179 708
Size: 179 529
BssHII G'CGCG_C
Cuts at: 0 74 172 708
Size: 74 98 536
Fragments arranged by size:
536 98 74
BstAPI GCAn_nnn'nTGC
Cuts at: 0 98 708
Size: 98 610
BstXI CCAn_nnnn'nTGG
Cuts at: 0 192 708
Size: 192 516
BstYI r'GATC_y
Cuts at: 0 383 530 708
Size: 383 147 178
Fragments arranged by size:
383 178 147
Bsu36I CC'TnA_GG
Cuts at: 0 439 708
Size: 439 269
BtgI C'CryG_G
Cuts at: 0 7 61 122 708
Size: 7 54 61 586
Fragments arranged by size:
586 61 54 7
BtsI GCAGTG_nn'
Cuts at: 0 185 708
Size: 185 523
EaeI y'GGCC_r
Cuts at: 0 521 708
Size: 521 187
EcoICRI GAG'CTC
Cuts at: 0 229 246 708
Size: 229 17 462
Fragments arranged by size:
462 229 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 655 708
Size: 312 343 53
Fragments arranged by size:
343 312 53
EcoO109I rG'GnC_Cy
Cuts at: 0 45 489 490 708
Size: 45 444 1 218
Fragments arranged by size:
444 218 45 1
FspI TGC'GCA
Cuts at: 0 238 664 708
Size: 238 426 44
Fragments arranged by size:
426 238 44
FspAI rTGC'GCAy
Cuts at: 0 238 664 708
Size: 238 426 44
Fragments arranged by size:
426 238 44
HaeII r_GCGC'y
Cuts at: 0 122 409 708
Size: 122 287 299
Fragments arranged by size:
299 287 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 708
Size: 187 32 489
Fragments arranged by size:
489 187 32
HincII GTy'rAC
Cuts at: 0 453 708
Size: 453 255
KasI G'GCGC_C
Cuts at: 0 118 405 708
Size: 118 287 303
Fragments arranged by size:
303 287 118
KpnI G_GTAC'C
Cuts at: 0 168 708
Size: 168 540
MspA1I CmG'CkG
Cuts at: 0 9 63 259 468 677 683 708
Size: 9 54 196 209 209 6 25
Fragments arranged by size:
209 209 196 54 25 9 6
NarI GG'CG_CC
Cuts at: 0 119 406 708
Size: 119 287 302
Fragments arranged by size:
302 287 119
PspOMI G'GGCC_C
Cuts at: 0 44 489 708
Size: 44 445 219
Fragments arranged by size:
445 219 44
PvuII CAG'CTG
Cuts at: 0 468 708
Size: 468 240
SacI G_AGCT'C
Cuts at: 0 231 248 708
Size: 231 17 460
Fragments arranged by size:
460 231 17
SacII CC_GC'GG
Cuts at: 0 10 64 708
Size: 10 54 644
Fragments arranged by size:
644 54 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 488 708
Size: 488 220
SfoI GGC'GCC
Cuts at: 0 120 407 708
Size: 120 287 301
Fragments arranged by size:
301 287 120
SmlI C'TyrA_G
Cuts at: 0 550 708
Size: 550 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 293 442 708
Size: 293 149 266
Fragments arranged by size:
293 266 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 218 250 708
Size: 218 32 458
Fragments arranged by size:
458 218 32
Tth111I GACn'n_nGTC
Cuts at: 0 448 708
Size: 448 260
XmnI GAAnn'nnTTC
Cuts at: 0 691 694 708
Size: 691 3 14
Fragments arranged by size:
691 14 3
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApaI AvaI BaeI BanI
BanII BbeI BbsI BcgI BciVI BglI BlpI Bme1580I
BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EcoICRI
Eco57MI EcoO109I FspI FspAI HaeII Hin4I HincII KasI
KpnI MspA1I NarI PspOMI PvuII SacI SacII SfiI
SfoI SmlI TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbvCI BclI BfrBI BglII BmgBI BmrI
BmtI BplI BsaI BsaAI BsaBI BseRI BsiEI BsiWI
BsmBI BspEI BspHI BsrGI BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoNI EcoRI EcoRV FalI FseI HindIII HpaI
MfeI MluI MmeI MscI MslI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI XbaI XcmI XhoI XmaI ZraI