Restriction map for
pFN21AE4026
(Linear) (Six-base) MAPSORT of: KIEE4026.seq Check: 6618 from: 1 to: 702
KIEE4026.orf
With 182 enzymes: SgfI *
March 22, 2012 17:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 441 702
Size: 441 261
AfeI AGC'GCT
Cuts at: 0 87 702
Size: 87 615
AhdI GACnn_n'nnGTC
Cuts at: 0 145 702
Size: 145 557
AlwNI CAG_nnn'CTG
Cuts at: 0 183 702
Size: 183 519
ApoI r'AATT_y
Cuts at: 0 379 702
Size: 379 323
AseI AT'TA_AT
Cuts at: 0 400 702
Size: 400 302
AvaI C'yCGr_G
Cuts at: 0 35 702
Size: 35 667
BanI G'GyrC_C
Cuts at: 0 97 154 262 453 702
Size: 97 57 108 191 249
Fragments arranged by size:
249 191 108 97 57
BanII G_rGCy'C
Cuts at: 0 23 95 503 659 702
Size: 23 72 408 156 43
Fragments arranged by size:
408 156 72 43 23
BbeI G_GCGC'C
Cuts at: 0 101 266 702
Size: 101 165 436
Fragments arranged by size:
436 165 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 593 702
Size: 593 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 702
Size: 678 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 275 702
Size: 275 427
BsaHI Gr'CG_yC
Cuts at: 0 98 263 702
Size: 98 165 439
Fragments arranged by size:
439 165 98
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 508 511 555 702
Size: 508 3 44 147
Fragments arranged by size:
508 147 44 3
BseYI C'CCAG_C
Cuts at: 0 57 88 107 658 702
Size: 57 31 19 551 44
Fragments arranged by size:
551 57 44 31 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 702
Size: 610 92
BsiHKAI G_wGCw'C
Cuts at: 0 23 503 702
Size: 23 480 199
Fragments arranged by size:
480 199 23
BsiWI C'GTAC_G
Cuts at: 0 638 702
Size: 638 64
Bsp1286I G_dGCh'C
Cuts at: 0 23 95 503 659 702
Size: 23 72 408 156 43
Fragments arranged by size:
408 156 72 43 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 441 702
Size: 441 261
BsrFI r'CCGG_y
Cuts at: 0 30 702
Size: 30 672
BstXI CCAn_nnnn'nTGG
Cuts at: 0 115 702
Size: 115 587
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 431 702
Size: 431 271
BtsI GCAGTG_nn'
Cuts at: 0 12 490 702
Size: 12 478 212
Fragments arranged by size:
478 212 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 276 311 702
Size: 276 35 391
Fragments arranged by size:
391 276 35
EaeI y'GGCC_r
Cuts at: 0 647 702
Size: 647 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 702
Size: 19 683
EcoICRI GAG'CTC
Cuts at: 0 21 501 702
Size: 21 480 201
Fragments arranged by size:
480 201 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 352 702
Size: 352 350
EcoO109I rG'GnC_Cy
Cuts at: 0 192 702
Size: 192 510
HaeII r_GCGC'y
Cuts at: 0 89 101 114 266 702
Size: 89 12 13 152 436
Fragments arranged by size:
436 152 89 13 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 131 163 184 216 226 258
Size: 10 32 89 32 21 32 10 32
Cuts at: 258 702
Size: 444
Fragments arranged by size:
444 89 32 32 32 32 21 10
10
KasI G'GCGC_C
Cuts at: 0 97 262 702
Size: 97 165 440
Fragments arranged by size:
440 165 97
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 347 551 702
Size: 162 185 204 151
Fragments arranged by size:
204 185 162 151
MslI CAynn'nnrTG
Cuts at: 0 508 702
Size: 508 194
MspA1I CmG'CkG
Cuts at: 0 80 702
Size: 80 622
NaeI GCC'GGC
Cuts at: 0 32 702
Size: 32 670
NarI GG'CG_CC
Cuts at: 0 98 263 702
Size: 98 165 439
Fragments arranged by size:
439 165 98
NgoMIV G'CCGG_C
Cuts at: 0 30 702
Size: 30 672
NspI r_CATG'y
Cuts at: 0 469 702
Size: 469 233
PpuMI rG'GwC_Cy
Cuts at: 0 192 702
Size: 192 510
PstI C_TGCA'G
Cuts at: 0 85 485 702
Size: 85 400 217
Fragments arranged by size:
400 217 85
SacI G_AGCT'C
Cuts at: 0 23 503 702
Size: 23 480 199
Fragments arranged by size:
480 199 23
SanDI GG'GwC_CC
Cuts at: 0 192 702
Size: 192 510
SfcI C'TryA_G
Cuts at: 0 81 481 702
Size: 81 400 221
Fragments arranged by size:
400 221 81
SfoI GGC'GCC
Cuts at: 0 99 264 702
Size: 99 165 438
Fragments arranged by size:
438 165 99
SmaI CCC'GGG
Cuts at: 0 37 702
Size: 37 665
SmlI C'TyrA_G
Cuts at: 0 693 702
Size: 693 9
StyI C'CwwG_G
Cuts at: 0 617 702
Size: 617 85
XmaI C'CCGG_G
Cuts at: 0 35 702
Size: 35 667
XmnI GAAnn'nnTTC
Cuts at: 0 202 702
Size: 202 500
Enzymes that do cut:
AarI AfeI AhdI AlwNI ApoI AseI AvaI BanI
BanII BbeI BbsI BpuEI BsaI BsaHI BseRI BseYI
BsgI BsiHKAI BsiWI Bsp1286I BspMI BsrFI BstXI BtgZI
BtsI CspCI EaeI EciI EcoICRI EcoNI EcoO109I HaeII
Hin4I KasI MmeI MslI MspA1I NaeI NarI NgoMIV
NspI PpuMI PstI SacI SanDI SfcI SfoI SmaI
SmlI StyI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaAI BsaBI BsaWI BsaXI BsiEI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI ClaI DraI
DraIII DrdI EagI EarI Eco57MI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI ZraI