Restriction map for
pFN21AE4024
(Linear) (Six-base) MAPSORT of: KIEE4024.seq Check: 4544 from: 1 to: 681
KIEE4024.orf
With 182 enzymes: SgfI *
March 22, 2012 17:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 250 681
Size: 250 431
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 681
Size: 220 461
AleI CACnn'nnGTG
Cuts at: 0 67 681
Size: 67 614
AlwNI CAG_nnn'CTG
Cuts at: 0 366 617 681
Size: 366 251 64
Fragments arranged by size:
366 251 64
ApaI G_GGCC'C
Cuts at: 0 512 681
Size: 512 169
AvaI C'yCGr_G
Cuts at: 0 274 522 605 681
Size: 274 248 83 76
Fragments arranged by size:
274 248 83 76
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 329 362 681
Size: 329 33 319
Fragments arranged by size:
329 319 33
BamHI G'GATC_C
Cuts at: 0 372 681
Size: 372 309
BanI G'GyrC_C
Cuts at: 0 458 681
Size: 458 223
BanII G_rGCy'C
Cuts at: 0 137 512 681
Size: 137 375 169
Fragments arranged by size:
375 169 137
BbeI G_GCGC'C
Cuts at: 0 462 681
Size: 462 219
Bme1580I G_kGCm'C
Cuts at: 0 512 681
Size: 512 169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 612 681
Size: 133 479 69
Fragments arranged by size:
479 133 69
BsaAI yAC'GTr
Cuts at: 0 447 681
Size: 447 234
BsaHI Gr'CG_yC
Cuts at: 0 459 681
Size: 459 222
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 615 681
Size: 615 66
BseYI C'CCAG_C
Cuts at: 0 29 79 329 400 598 681
Size: 29 50 250 71 198 83
Fragments arranged by size:
250 198 83 71 50 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 681
Size: 238 443
BsiHKAI G_wGCw'C
Cuts at: 0 545 681
Size: 545 136
Bsp1286I G_dGCh'C
Cuts at: 0 137 512 545 681
Size: 137 375 33 136
Fragments arranged by size:
375 137 136 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 250 681
Size: 250 431
BsrDI GCAATG_nn'
Cuts at: 0 674 681
Size: 674 7
BstEII G'GTnAC_C
Cuts at: 0 261 681
Size: 261 420
BstXI CCAn_nnnn'nTGG
Cuts at: 0 69 165 408 681
Size: 69 96 243 273
Fragments arranged by size:
273 243 96 69
BstYI r'GATC_y
Cuts at: 0 372 681
Size: 372 309
Bsu36I CC'TnA_GG
Cuts at: 0 359 681
Size: 359 322
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 540 681
Size: 540 141
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 428 463 681
Size: 428 35 218
Fragments arranged by size:
428 218 35
EaeI y'GGCC_r
Cuts at: 0 464 681
Size: 464 217
EarI CTCTTCn'nnn_
Cuts at: 0 589 643 675 681
Size: 589 54 32 6
Fragments arranged by size:
589 54 32 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 220 612 681
Size: 133 87 392 69
Fragments arranged by size:
392 133 87 69
EcoNI CCTnn'n_nnAGG
Cuts at: 0 627 681
Size: 627 54
EcoO109I rG'GnC_Cy
Cuts at: 0 279 509 681
Size: 279 230 172
Fragments arranged by size:
279 230 172
HaeII r_GCGC'y
Cuts at: 0 462 681
Size: 462 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 527 559 681
Size: 527 32 122
Fragments arranged by size:
527 122 32
KasI G'GCGC_C
Cuts at: 0 458 681
Size: 458 223
MslI CAynn'nnrTG
Cuts at: 0 67 681
Size: 67 614
MspA1I CmG'CkG
Cuts at: 0 67 143 249 324 614 681
Size: 67 76 106 75 290 67
Fragments arranged by size:
290 106 76 75 67 67
NarI GG'CG_CC
Cuts at: 0 459 681
Size: 459 222
NspI r_CATG'y
Cuts at: 0 181 681
Size: 181 500
PfoI T'CCnGG_A
Cuts at: 0 628 681
Size: 628 53
PmlI CAC'GTG
Cuts at: 0 447 681
Size: 447 234
PspOMI G'GGCC_C
Cuts at: 0 508 681
Size: 508 173
PstI C_TGCA'G
Cuts at: 0 292 646 681
Size: 292 354 35
Fragments arranged by size:
354 292 35
PvuII CAG'CTG
Cuts at: 0 143 324 614 681
Size: 143 181 290 67
Fragments arranged by size:
290 181 143 67
SexAI A'CCwGG_T
Cuts at: 0 229 681
Size: 229 452
SfcI C'TryA_G
Cuts at: 0 138 288 642 681
Size: 138 150 354 39
Fragments arranged by size:
354 150 138 39
SfoI GGC'GCC
Cuts at: 0 460 681
Size: 460 221
SmaI CCC'GGG
Cuts at: 0 276 524 607 681
Size: 276 248 83 74
Fragments arranged by size:
276 248 83 74
SphI G_CATG'C
Cuts at: 0 181 681
Size: 181 500
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 528 681
Size: 528 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 655 681
Size: 655 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 66 681
Size: 66 615
XmaI C'CCGG_G
Cuts at: 0 274 522 605 681
Size: 274 248 83 76
Fragments arranged by size:
274 248 83 76
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI AvaI BaeI BamHI
BanI BanII BbeI Bme1580I BpmI BsaAI BsaHI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BstEII BstXI
BstYI Bsu36I BtgZI CspCI EaeI EarI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MslI MspA1I NarI NspI
PfoI PmlI PspOMI PstI PvuII SexAI SfcI SfoI
SmaI SphI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I BtgI BtsI ClaI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI