(Linear) (Six-base) MAPSORT of: KIEE4022.seq  Check: 3079  from: 1  to: 630



KIEE4022.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:10  ..

Acc65I G'GTAC_C

Cuts at:      0     575     630 
   Size:        575      55
 
AvaI C'yCGr_G

Cuts at:      0     145     556     610     630 
   Size:        145     411      54      20

  Fragments arranged by size: 

                 411     145      54      20
 
BanI G'GyrC_C

Cuts at:      0     575     630 
   Size:        575      55
 
Bme1580I G_kGCm'C

Cuts at:      0     186     586     630 
   Size:        186     400      44

  Fragments arranged by size: 

                 400     186      44
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     399     630 
   Size:        208     191     231

  Fragments arranged by size: 

                 231     208     191
 
BseYI C'CCAG_C

Cuts at:      0      85     630 
   Size:         85     545
 
BsiHKAI G_wGCw'C

Cuts at:      0     271     630 
   Size:        271     359
 
Bsp1286I G_dGCh'C

Cuts at:      0     186     271     586     630 
   Size:        186      85     315      44

  Fragments arranged by size: 

                 315     186      85      44
 
BspHI T'CATG_A

Cuts at:      0     389     630 
   Size:        389     241
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      35     105     630 
   Size:         35      70     525

  Fragments arranged by size: 

                 525      70      35
 
BssSI C'ACGA_G

Cuts at:      0     520     630 
   Size:        520     110
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     240     630 
   Size:        240     390
 
BtsI GCAGTG_nn'

Cuts at:      0     101     426     630 
   Size:        101     325     204

  Fragments arranged by size: 

                 325     204     101
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     399     630 
   Size:        208     191     231

  Fragments arranged by size: 

                 231     208     191
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15     410     630 
   Size:         15     395     220

  Fragments arranged by size: 

                 395     220      15
 
HaeII r_GCGC'y

Cuts at:      0     169     630 
   Size:        169     461
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     476     508     630 
   Size:        476      32     122

  Fragments arranged by size: 

                 476     122      32
 
KpnI G_GTAC'C

Cuts at:      0     579     630 
   Size:        579      51
 
MslI CAynn'nnrTG

Cuts at:      0     520     597     630 
   Size:        520      77      33

  Fragments arranged by size: 

                 520      77      33
 
PasI CC'CwG_GG

Cuts at:      0      36     630 
   Size:         36     594
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     230     630 
   Size:        230     400
 
PpuMI rG'GwC_Cy

Cuts at:      0      15     410     630 
   Size:         15     395     220

  Fragments arranged by size: 

                 395     220      15
 
PstI C_TGCA'G

Cuts at:      0     461     630 
   Size:        461     169
 
SfcI C'TryA_G

Cuts at:      0     457     630 
   Size:        457     173
 
SmaI CCC'GGG

Cuts at:      0     558     630 
   Size:        558      72
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     591     630 
   Size:        591      39
 
XmaI C'CCGG_G

Cuts at:      0     556     630 
   Size:        556      74
 

 Enzymes that do cut:

   Acc65I     AvaI     BanI Bme1580I     BpmI    BseYI  BsiHKAI Bsp1286I
    BspHI    BspMI    BssSI   BstAPI     BtsI  Eco57MI EcoO109I    HaeII
    Hin4I     KpnI     MslI     PasI    PflMI    PpuMI     PstI     SfcI
     SmaI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI