(Linear) (Six-base) MAPSORT of: KIEE4021.seq  Check: 711  from: 1  to: 627



KIEE4021.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535     627 
   Size:        535      92
 
ApoI r'AATT_y

Cuts at:      0       4     190     627 
   Size:          4     186     437

  Fragments arranged by size: 

                 437     186       4
 
AvaI C'yCGr_G

Cuts at:      0     594     627 
   Size:        594      33
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     595     627 
   Size:        595      32
 
BclI T'GATC_A

Cuts at:      0     369     627 
   Size:        369     258
 
BlpI GC'TnA_GC

Cuts at:      0     233     627 
   Size:        233     394
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      70     627 
   Size:         70     557
 
BmtI G_CTAG'C

Cuts at:      0      44     627 
   Size:         44     583
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     451     571     627 
   Size:        226     225     120      56

  Fragments arranged by size: 

                 226     225     120      56
 
BseYI C'CCAG_C

Cuts at:      0     206     210     627 
   Size:        206       4     417

  Fragments arranged by size: 

                 417     206       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     375     576     627 
   Size:        375     201      51

  Fragments arranged by size: 

                 375     201      51
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      57     627 
   Size:         57     570
 
BstYI r'GATC_y

Cuts at:      0     135     627 
   Size:        135     492
 
BtgI C'CryG_G

Cuts at:      0     448     627 
   Size:        448     179
 
EarI CTCTTCn'nnn_

Cuts at:      0     253     259     269     627 
   Size:        253       6      10     358

  Fragments arranged by size: 

                 358     253      10       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     577     627 
   Size:        577      50
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     451     535     571     627 
   Size:        226     225      84      36      56

  Fragments arranged by size: 

                 226     225      84      56      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     417     627 
   Size:        417     210
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     626     627 
   Size:        626       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     516     548     558     590     627 
   Size:        516      32      10      32      37

  Fragments arranged by size: 

                 516      37      32      32      10
 
MslI CAynn'nnrTG

Cuts at:      0     121     383     487     627 
   Size:        121     262     104     140

  Fragments arranged by size: 

                 262     140     121     104
 
MspA1I CmG'CkG

Cuts at:      0      89     210     627 
   Size:         89     121     417

  Fragments arranged by size: 

                 417     121      89
 
NheI G'CTAG_C

Cuts at:      0      40     627 
   Size:         40     587
 
NspI r_CATG'y

Cuts at:      0     286     627 
   Size:        286     341
 
PfoI T'CCnGG_A

Cuts at:      0     127     627 
   Size:        127     500
 
PsiI TTA'TAA

Cuts at:      0     196     627 
   Size:        196     431
 
PvuII CAG'CTG

Cuts at:      0      89     210     627 
   Size:         89     121     417

  Fragments arranged by size: 

                 417     121      89
 
SmaI CCC'GGG

Cuts at:      0     596     627 
   Size:        596      31
 
StyI C'CwwG_G

Cuts at:      0     349     627 
   Size:        349     278
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     504     627 
   Size:        504     123
 
XmaI C'CCGG_G

Cuts at:      0     594     627 
   Size:        594      33
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI     BcgI     BclI     BlpI     BmrI     BmtI
     BpmI    BseYI     BsgI    BstXI    BstYI     BtgI     EarI     EciI
  Eco57MI    EcoNI     FalI    Hin4I     MslI   MspA1I     NheI     NspI
     PfoI     PsiI    PvuII     SmaI     StyI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmnI     ZraI