(Linear) (Six-base) MAPSORT of: KIEE4020.seq  Check: 7494  from: 1  to: 615



KIEE4020.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:09  ..

AatII G_ACGT'C

Cuts at:      0     549     615 
   Size:        549      66
 
AfeI AGC'GCT

Cuts at:      0     290     615 
   Size:        290     325
 
AlwNI CAG_nnn'CTG

Cuts at:      0     474     615 
   Size:        474     141
 
ApoI r'AATT_y

Cuts at:      0     199     615 
   Size:        199     416
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     260     615 
   Size:        260     355
 
BclI T'GATC_A

Cuts at:      0     578     615 
   Size:        578      37
 
BglII A'GATC_T

Cuts at:      0     375     615 
   Size:        375     240
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     234     545     615 
   Size:        234     311      70

  Fragments arranged by size: 

                 311     234      70
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     615 
   Size:        496     119
 
BsaBI GATnn'nnATC

Cuts at:      0     493     561     615 
   Size:        493      68      54

  Fragments arranged by size: 

                 493      68      54
 
BsaHI Gr'CG_yC

Cuts at:      0     546     615 
   Size:        546      69
 
BseYI C'CCAG_C

Cuts at:      0     401     615 
   Size:        401     214
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     537     615 
   Size:        537      78
 
BstYI r'GATC_y

Cuts at:      0     375     556     615 
   Size:        375     181      59

  Fragments arranged by size: 

                 375     181      59
 
BtsI GCAGTG_nn'

Cuts at:      0     356     615 
   Size:        356     259
 
EaeI y'GGCC_r

Cuts at:      0     569     615 
   Size:        569      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     615 
   Size:        496     119
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     147     615 
   Size:        147     468
 
HaeII r_GCGC'y

Cuts at:      0     292     615 
   Size:        292     323
 
MscI TGG'CCA

Cuts at:      0     571     615 
   Size:        571      44
 
PfoI T'CCnGG_A

Cuts at:      0     305     615 
   Size:        305     310
 
PstI C_TGCA'G

Cuts at:      0     462     615 
   Size:        462     153
 
SfcI C'TryA_G

Cuts at:      0     297     458     615 
   Size:        297     161     157

  Fragments arranged by size: 

                 297     161     157
 
SpeI A'CTAG_T

Cuts at:      0     319     615 
   Size:        319     296
 
XbaI T'CTAG_A

Cuts at:      0     159     615 
   Size:        159     456
 
ZraI GAC'GTC

Cuts at:      0     547     615 
   Size:        547      68
 

 Enzymes that do cut:

    AatII     AfeI    AlwNI     ApoI     BbsI     BclI    BglII     BmrI
     BpmI    BsaBI    BsaHI    BseYI    BsmBI    BstYI     BtsI     EaeI
  Eco57MI     FalI    HaeII     MscI     PfoI     PstI     SfcI     SpeI
     XbaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI