(Linear) (Six-base) MAPSORT of: KIEE4015.seq  Check: 8036  from: 1  to: 579



KIEE4015.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      28     579 
   Size:         28     551
 
AhdI GACnn_n'nnGTC

Cuts at:      0     210     579 
   Size:        210     369
 
AleI CACnn'nnGTG

Cuts at:      0     371     443     455     579 
   Size:        371      72      12     124

  Fragments arranged by size: 

                 371     124      72      12
 
ApaLI G'TGCA_C

Cuts at:      0     448     579 
   Size:        448     131
 
ApoI r'AATT_y

Cuts at:      0     297     579 
   Size:        297     282
 
AvaI C'yCGr_G

Cuts at:      0     150     579 
   Size:        150     429
 
BanI G'GyrC_C

Cuts at:      0     542     579 
   Size:        542      37
 
BbvCI CC'TCA_GC

Cuts at:      0     142     579 
   Size:        142     437
 
BlpI GC'TnA_GC

Cuts at:      0      82     579 
   Size:         82     497
 
Bme1580I G_kGCm'C

Cuts at:      0     452     579 
   Size:        452     127
 
Bpu10I CC'TnA_GC

Cuts at:      0       5     142     579 
   Size:          5     137     437

  Fragments arranged by size: 

                 437     137       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     151     452     579 
   Size:        151     301     127

  Fragments arranged by size: 

                 301     151     127
 
BsmI GAATG_Cn'

Cuts at:      0     394     579 
   Size:        394     185
 
Bsp1286I G_dGCh'C

Cuts at:      0     151     452     579 
   Size:        151     301     127

  Fragments arranged by size: 

                 301     151     127
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      28     115     579 
   Size:         28      87     464

  Fragments arranged by size: 

                 464      87      28
 
BsrBI CCG'CTC

Cuts at:      0     120     579 
   Size:        120     459
 
BstEII G'GTnAC_C

Cuts at:      0     160     579 
   Size:        160     419
 
BtsI GCAGTG_nn'

Cuts at:      0      56     312     579 
   Size:         56     256     267

  Fragments arranged by size: 

                 267     256      56
 
ClaI AT'CG_AT

Cuts at:      0     510     579 
   Size:        510      69
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     144     176     196     228     579 
   Size:        144      32      20      32     351

  Fragments arranged by size: 

                 351     144      32      32      20
 
MslI CAynn'nnrTG

Cuts at:      0     371     387     443     455     579 
   Size:        371      16      56      12     124

  Fragments arranged by size: 

                 371     124      56      16      12
 
PfoI T'CCnGG_A

Cuts at:      0     184     579 
   Size:        184     395
 
PspXI vC'TCGA_Gb

Cuts at:      0     150     579 
   Size:        150     429
 
PstI C_TGCA'G

Cuts at:      0      63     579 
   Size:         63     516
 
SfcI C'TryA_G

Cuts at:      0      59     579 
   Size:         59     520
 
SmlI C'TyrA_G

Cuts at:      0     150     579 
   Size:        150     429
 
StuI AGG'CCT

Cuts at:      0      32     220     579 
   Size:         32     188     359

  Fragments arranged by size: 

                 359     188      32
 
StyI C'CwwG_G

Cuts at:      0     537     579 
   Size:        537      42
 
XhoI C'TCGA_G

Cuts at:      0     150     579 
   Size:        150     429
 

 Enzymes that do cut:

     AarI     AhdI     AleI    ApaLI     ApoI     AvaI     BanI    BbvCI
     BlpI Bme1580I   Bpu10I  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI
   BstEII     BtsI     ClaI    Hin4I     MslI     PfoI    PspXI     PstI
     SfcI     SmlI     StuI     StyI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI