(Linear) (Six-base) MAPSORT of: KIEE4010.seq  Check: 9348  from: 1  to: 489



KIEE4010.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:08  ..

AflII C'TTAA_G

Cuts at:      0     414     489 
   Size:        414      75
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      91     123     489 
   Size:         91      32     366

  Fragments arranged by size: 

                 366      91      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     243     489 
   Size:        243     246
 
ApoI r'AATT_y

Cuts at:      0      58     126     489 
   Size:         58      68     363

  Fragments arranged by size: 

                 363      68      58
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     164     489 
   Size:        164     325
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     489 
   Size:        169     320
 
BsaHI Gr'CG_yC

Cuts at:      0       5     489 
   Size:          5     484
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      30     489 
   Size:         30     459
 
BtsI GCAGTG_nn'

Cuts at:      0     426     489 
   Size:        426      63
 
DraI TTT'AAA

Cuts at:      0     363     489 
   Size:        363     126
 
EarI CTCTTCn'nnn_

Cuts at:      0     161     489 
   Size:        161     328
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      33     489 
   Size:         33     456
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     489 
   Size:        169     320
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     489 
   Size:         54     435
 
MslI CAynn'nnrTG

Cuts at:      0      94     302     489 
   Size:         94     208     187

  Fragments arranged by size: 

                 208     187      94
 
MspA1I CmG'CkG

Cuts at:      0     240     249     489 
   Size:        240       9     240

  Fragments arranged by size: 

                 240     240       9
 
NspI r_CATG'y

Cuts at:      0     333     489 
   Size:        333     156
 
PstI C_TGCA'G

Cuts at:      0     248     489 
   Size:        248     241
 
PvuII CAG'CTG

Cuts at:      0     240     489 
   Size:        240     249
 
ScaI AGT'ACT

Cuts at:      0     142     489 
   Size:        142     347
 
SfcI C'TryA_G

Cuts at:      0     244     381     489 
   Size:        244     137     108

  Fragments arranged by size: 

                 244     137     108
 
SmlI C'TyrA_G

Cuts at:      0     414     489 
   Size:        414      75
 
TatI w'GTAC_w

Cuts at:      0     140     489 
   Size:        140     349
 

 Enzymes that do cut:

    AflII     AloI    AlwNI     ApoI     BbsI     BpmI    BsaHI    BseRI
     BtsI     DraI     EarI     EciI  Eco57MI     MmeI     MslI   MspA1I
     NspI     PstI    PvuII     ScaI     SfcI     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI