(Linear) (Six-base) MAPSORT of: KIEE4007.seq  Check: 5579  from: 1  to: 465



KIEE4007.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:07  ..

BbsI GAAGACnn'nnnn_

Cuts at:      0     135     299     465 
   Size:        135     164     166

  Fragments arranged by size: 

                 166     164     135
 
BlpI GC'TnA_GC

Cuts at:      0     151     465 
   Size:        151     314
 
BsiHKAI G_wGCw'C

Cuts at:      0     326     465 
   Size:        326     139
 
Bsp1286I G_dGCh'C

Cuts at:      0     326     465 
   Size:        326     139
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     283     465 
   Size:        283     182
 
BsrBI CCG'CTC

Cuts at:      0     102     465 
   Size:        102     363
 
BsrDI GCAATG_nn'

Cuts at:      0     407     465 
   Size:        407      58
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      14     465 
   Size:         14     451
 
DraIII CAC_nnn'GTG

Cuts at:      0     205     465 
   Size:        205     260
 
FspI TGC'GCA

Cuts at:      0     279     465 
   Size:        279     186
 
MslI CAynn'nnrTG

Cuts at:      0      12     112     465 
   Size:         12     100     353

  Fragments arranged by size: 

                 353     100      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      20      93     465 
   Size:         20      73     372

  Fragments arranged by size: 

                 372      73      20
 

 Enzymes that do cut:

     BbsI     BlpI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BstXI
   DraIII     FspI     MslI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI