(Linear) (Six-base) MAPSORT of: KIEE4004.seq  Check: 2625  from: 1  to: 291



KIEE4004.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:07  ..

AfeI AGC'GCT

Cuts at:      0      91     205     291 
   Size:         91     114      86

  Fragments arranged by size: 

                 114      91      86
 
AlwNI CAG_nnn'CTG

Cuts at:      0     102     291 
   Size:        102     189
 
ApaLI G'TGCA_C

Cuts at:      0     106     291 
   Size:        106     185
 
BbvCI CC'TCA_GC

Cuts at:      0     176     291 
   Size:        176     115
 
Bme1580I G_kGCm'C

Cuts at:      0     110     291 
   Size:        110     181
 
Bpu10I CC'TnA_GC

Cuts at:      0     176     291 
   Size:        176     115
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       3     291 
   Size:          3     288
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     291 
   Size:         59     232
 
BsiHKAI G_wGCw'C

Cuts at:      0     110     291 
   Size:        110     181
 
Bsp1286I G_dGCh'C

Cuts at:      0     110     291 
   Size:        110     181
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     105     291 
   Size:        105     186
 
BtgI C'CryG_G

Cuts at:      0      23     291 
   Size:         23     268
 
BtsI GCAGTG_nn'

Cuts at:      0      47     291 
   Size:         47     244
 
EarI CTCTTCn'nnn_

Cuts at:      0      21     291 
   Size:         21     270
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     108     291 
   Size:        108     183
 
HaeII r_GCGC'y

Cuts at:      0      93     207     291 
   Size:         93     114      84

  Fragments arranged by size: 

                 114      93      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     291 
   Size:         27     264
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     291 
   Size:        143     148
 
MspA1I CmG'CkG

Cuts at:      0      25     102     291 
   Size:         25      77     189

  Fragments arranged by size: 

                 189      77      25
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     102     291 
   Size:        102     189
 
PvuII CAG'CTG

Cuts at:      0     102     291 
   Size:        102     189
 
SacII CC_GC'GG

Cuts at:      0      26     291 
   Size:         26     265
 
StuI AGG'CCT

Cuts at:      0     181     291 
   Size:        181     110
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      25     291 
   Size:         25     266
 

 Enzymes that do cut:

     AfeI    AlwNI    ApaLI    BbvCI Bme1580I   Bpu10I    BseRI     BsgI
  BsiHKAI Bsp1286I   BstAPI     BtgI     BtsI     EarI     FalI    HaeII
    Hin4I     MmeI   MspA1I    PflMI    PvuII    SacII     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI