Restriction map for
pFN21AE4003
(Linear) (Six-base) MAPSORT of: KIEE4003.seq Check: 8855 from: 1 to: 798
KIEE4003.orf
With 182 enzymes: SgfI *
March 22, 2012 17:06 ..
AccI GT'mk_AC
Cuts at: 0 299 798
Size: 299 499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 798
Size: 706 92
AfeI AGC'GCT
Cuts at: 0 256 798
Size: 256 542
AlwNI CAG_nnn'CTG
Cuts at: 0 248 798
Size: 248 550
ApaI G_GGCC'C
Cuts at: 0 273 609 744 798
Size: 273 336 135 54
Fragments arranged by size:
336 273 135 54
AvaI C'yCGr_G
Cuts at: 0 55 737 798
Size: 55 682 61
Fragments arranged by size:
682 61 55
BanI G'GyrC_C
Cuts at: 0 80 470 759 798
Size: 80 390 289 39
Fragments arranged by size:
390 289 80 39
BanII G_rGCy'C
Cuts at: 0 273 571 609 744 798
Size: 273 298 38 135 54
Fragments arranged by size:
298 273 135 54 38
BbeI G_GCGC'C
Cuts at: 0 84 763 798
Size: 84 679 35
Fragments arranged by size:
679 84 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 516 798
Size: 516 282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 472 506 798
Size: 472 34 292
Fragments arranged by size:
472 292 34
BglII A'GATC_T
Cuts at: 0 791 798
Size: 791 7
Bme1580I G_kGCm'C
Cuts at: 0 273 609 744 798
Size: 273 336 135 54
Fragments arranged by size:
336 273 135 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 276 798
Size: 276 522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 580 798
Size: 358 222 218
Fragments arranged by size:
358 222 218
BsaI GGTCTCn'nnnn_
Cuts at: 0 492 798
Size: 492 306
BsaAI yAC'GTr
Cuts at: 0 513 798
Size: 513 285
BsaHI Gr'CG_yC
Cuts at: 0 81 760 798
Size: 81 679 38
Fragments arranged by size:
679 81 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 655 685 798
Size: 655 30 113
Fragments arranged by size:
655 113 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 613 798
Size: 193 420 185
Fragments arranged by size:
420 193 185
BseYI C'CCAG_C
Cuts at: 0 393 798
Size: 393 405
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 639 798
Size: 535 104 159
Fragments arranged by size:
535 159 104
BsiHKAI G_wGCw'C
Cuts at: 0 512 798
Size: 512 286
Bsp1286I G_dGCh'C
Cuts at: 0 273 512 571 609 744 798
Size: 273 239 59 38 135 54
Fragments arranged by size:
273 239 135 59 54 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 452 798
Size: 452 346
BsrBI CCG'CTC
Cuts at: 0 627 798
Size: 627 171
BsrGI T'GTAC_A
Cuts at: 0 290 798
Size: 290 508
BssHII G'CGCG_C
Cuts at: 0 47 798
Size: 47 751
BssSI C'ACGA_G
Cuts at: 0 529 798
Size: 529 269
BstEII G'GTnAC_C
Cuts at: 0 720 798
Size: 720 78
BstYI r'GATC_y
Cuts at: 0 791 798
Size: 791 7
Bsu36I CC'TnA_GG
Cuts at: 0 208 798
Size: 208 590
BtgI C'CryG_G
Cuts at: 0 84 798
Size: 84 714
BtsI GCAGTG_nn'
Cuts at: 0 221 542 629 798
Size: 221 321 87 169
Fragments arranged by size:
321 221 169 87
EaeI y'GGCC_r
Cuts at: 0 68 647 798
Size: 68 579 151
Fragments arranged by size:
579 151 68
EarI CTCTTCn'nnn_
Cuts at: 0 559 592 798
Size: 559 33 206
Fragments arranged by size:
559 206 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 580 706 798
Size: 358 222 126 92
Fragments arranged by size:
358 222 126 92
EcoO109I rG'GnC_Cy
Cuts at: 0 605 606 621 798
Size: 605 1 15 177
Fragments arranged by size:
605 177 15 1
HaeII r_GCGC'y
Cuts at: 0 84 258 763 798
Size: 84 174 505 35
Fragments arranged by size:
505 174 84 35
HincII GTy'rAC
Cuts at: 0 63 300 798
Size: 63 237 498
Fragments arranged by size:
498 237 63
KasI G'GCGC_C
Cuts at: 0 80 759 798
Size: 80 679 39
Fragments arranged by size:
679 80 39
MscI TGG'CCA
Cuts at: 0 70 649 798
Size: 70 579 149
Fragments arranged by size:
579 149 70
MspA1I CmG'CkG
Cuts at: 0 35 86 653 798
Size: 35 51 567 145
Fragments arranged by size:
567 145 51 35
NarI GG'CG_CC
Cuts at: 0 81 760 798
Size: 81 679 38
Fragments arranged by size:
679 81 38
NspI r_CATG'y
Cuts at: 0 523 798
Size: 523 275
PmlI CAC'GTG
Cuts at: 0 513 798
Size: 513 285
PspOMI G'GGCC_C
Cuts at: 0 269 605 740 798
Size: 269 336 135 58
Fragments arranged by size:
336 269 135 58
PspXI vC'TCGA_Gb
Cuts at: 0 55 798
Size: 55 743
PstI C_TGCA'G
Cuts at: 0 228 798
Size: 228 570
PvuII CAG'CTG
Cuts at: 0 653 798
Size: 653 145
SacII CC_GC'GG
Cuts at: 0 87 798
Size: 87 711
SalI G'TCGA_C
Cuts at: 0 298 798
Size: 298 500
SapI GCTCTTCn'nnn_
Cuts at: 0 559 798
Size: 559 239
SfcI C'TryA_G
Cuts at: 0 168 224 798
Size: 168 56 574
Fragments arranged by size:
574 168 56
SfoI GGC'GCC
Cuts at: 0 82 761 798
Size: 82 679 37
Fragments arranged by size:
679 82 37
SmaI CCC'GGG
Cuts at: 0 739 798
Size: 739 59
SmlI C'TyrA_G
Cuts at: 0 55 798
Size: 55 743
StyI C'CwwG_G
Cuts at: 0 434 798
Size: 434 364
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 458 798
Size: 458 340
TatI w'GTAC_w
Cuts at: 0 290 419 798
Size: 290 129 379
Fragments arranged by size:
379 290 129
XhoI C'TCGA_G
Cuts at: 0 55 798
Size: 55 743
XmaI C'CCGG_G
Cuts at: 0 737 798
Size: 737 61
XmnI GAAnn'nnTTC
Cuts at: 0 784 798
Size: 784 14
Enzymes that do cut:
AccI AcuI AfeI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BcgI BglII Bme1580I BmrI BpmI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrGI BssHII BssSI BstEII BstYI Bsu36I
BtgI BtsI EaeI EarI Eco57MI EcoO109I HaeII HincII
KasI MscI MspA1I NarI NspI PmlI PspOMI PspXI
PstI PvuII SacII SalI SapI SfcI SfoI SmaI
SmlI StyI TaqII TatI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglI
BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI
BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HindIII HpaI KpnI
MfeI MluI MmeI MslI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PvuI
RsrII SacI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI ZraI