Restriction map for
pFN21AE4002
(Linear) (Six-base) MAPSORT of: KIEE4002.seq Check: 2949 from: 1 to: 609
KIEE4002.orf
With 182 enzymes: SgfI *
July 3, 2012 18:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 238 609
Size: 238 371
AccI GT'mk_AC
Cuts at: 0 272 609
Size: 272 337
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 609
Size: 492 117
AflIII A'CryG_T
Cuts at: 0 405 425 609
Size: 405 20 184
Fragments arranged by size:
405 184 20
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 246 568 609
Size: 246 322 41
Fragments arranged by size:
322 246 41
AlwNI CAG_nnn'CTG
Cuts at: 0 330 609
Size: 330 279
ApaLI G'TGCA_C
Cuts at: 0 154 553 609
Size: 154 399 56
Fragments arranged by size:
399 154 56
Bme1580I G_kGCm'C
Cuts at: 0 158 557 609
Size: 158 399 52
Fragments arranged by size:
399 158 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 196 352 609
Size: 148 48 156 257
Fragments arranged by size:
257 156 148 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 609
Size: 505 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 9 609
Size: 9 600
BsaAI yAC'GTr
Cuts at: 0 153 465 552 609
Size: 153 312 87 57
Fragments arranged by size:
312 153 87 57
BsaHI Gr'CG_yC
Cuts at: 0 361 609
Size: 361 248
BsaWI w'CCGG_w
Cuts at: 0 245 609
Size: 245 364
BseYI C'CCAG_C
Cuts at: 0 27 609
Size: 27 582
BsiHKAI G_wGCw'C
Cuts at: 0 158 557 609
Size: 158 399 52
Fragments arranged by size:
399 158 52
BsmI GAATG_Cn'
Cuts at: 0 475 609
Size: 475 134
BsmBI CGTCTCn'nnnn_
Cuts at: 0 328 352 609
Size: 328 24 257
Fragments arranged by size:
328 257 24
Bsp1286I G_dGCh'C
Cuts at: 0 158 557 609
Size: 158 399 52
Fragments arranged by size:
399 158 52
BspEI T'CCGG_A
Cuts at: 0 245 609
Size: 245 364
BspHI T'CATG_A
Cuts at: 0 140 609
Size: 140 469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 238 401 609
Size: 134 104 163 208
Fragments arranged by size:
208 163 134 104
BsrGI T'GTAC_A
Cuts at: 0 74 609
Size: 74 535
BssHII G'CGCG_C
Cuts at: 0 578 609
Size: 578 31
BstAPI GCAn_nnn'nTGC
Cuts at: 0 498 596 609
Size: 498 98 13
Fragments arranged by size:
498 98 13
BstYI r'GATC_y
Cuts at: 0 167 326 609
Size: 167 159 283
Fragments arranged by size:
283 167 159
EaeI y'GGCC_r
Cuts at: 0 20 147 476 609
Size: 20 127 329 133
Fragments arranged by size:
329 133 127 20
EarI CTCTTCn'nnn_
Cuts at: 0 511 609
Size: 511 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 307 609
Size: 307 302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 196 352 492 609
Size: 148 48 156 140 117
Fragments arranged by size:
156 148 140 117 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 234 609
Size: 234 375
EcoO109I rG'GnC_Cy
Cuts at: 0 390 609
Size: 390 219
HaeII r_GCGC'y
Cuts at: 0 194 609
Size: 194 415
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 395 427 609
Size: 395 32 182
Fragments arranged by size:
395 182 32
MluI A'CGCG_T
Cuts at: 0 405 609
Size: 405 204
MslI CAynn'nnrTG
Cuts at: 0 373 609
Size: 373 236
MspA1I CmG'CkG
Cuts at: 0 596 609
Size: 596 13
PfoI T'CCnGG_A
Cuts at: 0 212 329 609
Size: 212 117 280
Fragments arranged by size:
280 212 117
PmlI CAC'GTG
Cuts at: 0 153 552 609
Size: 153 399 57
Fragments arranged by size:
399 153 57
PpuMI rG'GwC_Cy
Cuts at: 0 390 609
Size: 390 219
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 67 99 609
Size: 67 32 510
Fragments arranged by size:
510 67 32
PstI C_TGCA'G
Cuts at: 0 238 398 609
Size: 238 160 211
Fragments arranged by size:
238 211 160
SapI GCTCTTCn'nnn_
Cuts at: 0 511 609
Size: 511 98
SexAI A'CCwGG_T
Cuts at: 0 434 609
Size: 434 175
SfcI C'TryA_G
Cuts at: 0 234 394 609
Size: 234 160 215
Fragments arranged by size:
234 215 160
SmlI C'TyrA_G
Cuts at: 0 520 609
Size: 520 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 198 609
Size: 198 411
TatI w'GTAC_w
Cuts at: 0 74 482 609
Size: 74 408 127
Fragments arranged by size:
408 127 74
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 372 609
Size: 15 357 237
Fragments arranged by size:
357 237 15
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaLI Bme1580I
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BseYI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrGI BssHII
BstAPI BstYI EaeI EarI EciI Eco57MI EcoNI EcoO109I
HaeII Hin4I MluI MslI MspA1I PfoI PmlI PpuMI
PsrI PstI SapI SexAI SfcI SmlI TaqII TatI
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BsaBI BsaXI BseRI BsgI BsiEI
BsiWI BsrBI BsrDI BsrFI BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PspXI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI