(Linear) (Six-base) MAPSORT of: KIEE4001.seq  Check: 8364  from: 1  to: 558



KIEE4001.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     558 
   Size:         67     491
 
AfeI AGC'GCT

Cuts at:      0     250     558 
   Size:        250     308
 
AvaI C'yCGr_G

Cuts at:      0      90     558 
   Size:         90     468
 
BanII G_rGCy'C

Cuts at:      0      68     332     558 
   Size:         68     264     226

  Fragments arranged by size: 

                 264     226      68
 
BbvCI CC'TCA_GC

Cuts at:      0     353     558 
   Size:        353     205
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     452     486     558 
   Size:        452      34      72

  Fragments arranged by size: 

                 452      72      34
 
BglII A'GATC_T

Cuts at:      0     467     558 
   Size:        467      91
 
Bpu10I CC'TnA_GC

Cuts at:      0     353     558 
   Size:        353     205
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     389     558 
   Size:        389     169
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     275     558 
   Size:         70     205     283

  Fragments arranged by size: 

                 283     205      70
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     332     558 
   Size:         68     264     226

  Fragments arranged by size: 

                 264     226      68
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     332     558 
   Size:         68     264     226

  Fragments arranged by size: 

                 264     226      68
 
BsrGI T'GTAC_A

Cuts at:      0     278     558 
   Size:        278     280
 
BstYI r'GATC_y

Cuts at:      0     467     558 
   Size:        467      91
 
BtgI C'CryG_G

Cuts at:      0     155     558 
   Size:        155     403
 
EcoICRI GAG'CTC

Cuts at:      0      66     330     558 
   Size:         66     264     228

  Fragments arranged by size: 

                 264     228      66
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     558 
   Size:         67     491
 
EcoO109I rG'GnC_Cy

Cuts at:      0      39     558 
   Size:         39     519
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     120     558 
   Size:        120     438
 
FspI TGC'GCA

Cuts at:      0     172     558 
   Size:        172     386
 
HaeII r_GCGC'y

Cuts at:      0     252     272     558 
   Size:        252      20     286

  Fragments arranged by size: 

                 286     252      20
 
HincII GTy'rAC

Cuts at:      0     111     558 
   Size:        111     447
 
MspA1I CmG'CkG

Cuts at:      0     357     558 
   Size:        357     201
 
NspI r_CATG'y

Cuts at:      0     171     243     558 
   Size:        171      72     315

  Fragments arranged by size: 

                 315     171      72
 
PfoI T'CCnGG_A

Cuts at:      0     402     455     558 
   Size:        402      53     103

  Fragments arranged by size: 

                 402     103      53
 
PstI C_TGCA'G

Cuts at:      0     362     558 
   Size:        362     196
 
PvuII CAG'CTG

Cuts at:      0     357     558 
   Size:        357     201
 
SacI G_AGCT'C

Cuts at:      0      68     332     558 
   Size:         68     264     226

  Fragments arranged by size: 

                 264     226      68
 
ScaI AGT'ACT

Cuts at:      0      88     558 
   Size:         88     470
 
SfcI C'TryA_G

Cuts at:      0     358     520     558 
   Size:        358     162      38

  Fragments arranged by size: 

                 358     162      38
 
StuI AGG'CCT

Cuts at:      0     316     558 
   Size:        316     242
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     476     558 
   Size:        476      82
 
TatI w'GTAC_w

Cuts at:      0      86     278     558 
   Size:         86     192     280

  Fragments arranged by size: 

                 280     192      86
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     279     558 
   Size:        279     279
 

 Enzymes that do cut:

     AcuI     AfeI     AvaI    BanII    BbvCI     BcgI    BglII   Bpu10I
     BsaI    BseRI  BsiHKAI Bsp1286I    BsrGI    BstYI     BtgI  EcoICRI
  Eco57MI EcoO109I     FalI     FspI    HaeII   HincII   MspA1I     NspI
     PfoI     PstI    PvuII     SacI     ScaI     SfcI     StuI    TaqII
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI    EcoNI    EcoRI    EcoRV     FseI    FspAI    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI