Restriction map for
pFN21AE3996
(Linear) (Six-base) MAPSORT of: KIEE3996.seq Check: 9393 from: 1 to: 702
KIEE3996.orf
With 182 enzymes: SgfI *
March 22, 2012 17:06 ..
AatII G_ACGT'C
Cuts at: 0 358 599 702
Size: 358 241 103
Fragments arranged by size:
358 241 103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 702
Size: 375 327
AfeI AGC'GCT
Cuts at: 0 454 702
Size: 454 248
AflIII A'CryG_T
Cuts at: 0 77 194 693 702
Size: 77 117 499 9
Fragments arranged by size:
499 117 77 9
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 154 702
Size: 154 548
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 260 292 702
Size: 260 32 410
Fragments arranged by size:
410 260 32
ApaLI G'TGCA_C
Cuts at: 0 172 702
Size: 172 530
AvaI C'yCGr_G
Cuts at: 0 583 702
Size: 583 119
BanI G'GyrC_C
Cuts at: 0 58 479 702
Size: 58 421 223
Fragments arranged by size:
421 223 58
BbeI G_GCGC'C
Cuts at: 0 483 702
Size: 483 219
BbsI GAAGACnn'nnnn_
Cuts at: 0 570 702
Size: 570 132
Bme1580I G_kGCm'C
Cuts at: 0 176 702
Size: 176 526
BmgBI CAC'GTC
Cuts at: 0 645 702
Size: 645 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 412 469 702
Size: 222 190 57 233
Fragments arranged by size:
233 222 190 57
Bpu10I CC'TnA_GC
Cuts at: 0 340 702
Size: 340 362
BsaI GGTCTCn'nnnn_
Cuts at: 0 580 702
Size: 580 122
BsaHI Gr'CG_yC
Cuts at: 0 355 480 596 702
Size: 355 125 116 106
Fragments arranged by size:
355 125 116 106
BsaWI w'CCGG_w
Cuts at: 0 372 589 702
Size: 372 217 113
Fragments arranged by size:
372 217 113
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 702
Size: 220 30 452
Fragments arranged by size:
452 220 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 586 702
Size: 421 165 116
Fragments arranged by size:
421 165 116
BseYI C'CCAG_C
Cuts at: 0 146 702
Size: 146 556
BsiEI CG_ry'CG
Cuts at: 0 22 103 702
Size: 22 81 599
Fragments arranged by size:
599 81 22
BsiHKAI G_wGCw'C
Cuts at: 0 176 702
Size: 176 526
BsmBI CGTCTCn'nnnn_
Cuts at: 0 587 702
Size: 587 115
Bsp1286I G_dGCh'C
Cuts at: 0 176 702
Size: 176 526
BspEI T'CCGG_A
Cuts at: 0 372 702
Size: 372 330
BsrBI CCG'CTC
Cuts at: 0 18 26 537 660 702
Size: 18 8 511 123 42
Fragments arranged by size:
511 123 42 18 8
BsrFI r'CCGG_y
Cuts at: 0 55 619 674 702
Size: 55 564 55 28
Fragments arranged by size:
564 55 55 28
BsrGI T'GTAC_A
Cuts at: 0 197 702
Size: 197 505
BssHII G'CGCG_C
Cuts at: 0 412 702
Size: 412 290
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 279 702
Size: 210 69 423
Fragments arranged by size:
423 210 69
BtgI C'CryG_G
Cuts at: 0 62 434 702
Size: 62 372 268
Fragments arranged by size:
372 268 62
ClaI AT'CG_AT
Cuts at: 0 179 702
Size: 179 523
EaeI y'GGCC_r
Cuts at: 0 19 212 702
Size: 19 193 490
Fragments arranged by size:
490 193 19
EagI C'GGCC_G
Cuts at: 0 19 702
Size: 19 683
EarI CTCTTCn'nnn_
Cuts at: 0 259 702
Size: 259 443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 375 412 469 702
Size: 222 153 37 57 233
Fragments arranged by size:
233 222 153 57 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 568 702
Size: 568 134
EcoO109I rG'GnC_Cy
Cuts at: 0 29 561 633 702
Size: 29 532 72 69
Fragments arranged by size:
532 72 69 29
HaeII r_GCGC'y
Cuts at: 0 456 483 702
Size: 456 27 219
Fragments arranged by size:
456 219 27
HincII GTy'rAC
Cuts at: 0 615 702
Size: 615 87
KasI G'GCGC_C
Cuts at: 0 479 702
Size: 479 223
MscI TGG'CCA
Cuts at: 0 214 702
Size: 214 488
MslI CAynn'nnrTG
Cuts at: 0 179 181 702
Size: 179 2 521
Fragments arranged by size:
521 179 2
MspA1I CmG'CkG
Cuts at: 0 64 477 551 702
Size: 64 413 74 151
Fragments arranged by size:
413 151 74 64
NaeI GCC'GGC
Cuts at: 0 621 676 702
Size: 621 55 26
Fragments arranged by size:
621 55 26
NarI GG'CG_CC
Cuts at: 0 480 702
Size: 480 222
NcoI C'CATG_G
Cuts at: 0 434 702
Size: 434 268
NgoMIV G'CCGG_C
Cuts at: 0 619 674 702
Size: 619 55 28
Fragments arranged by size:
619 55 28
NspI r_CATG'y
Cuts at: 0 81 198 697 702
Size: 81 117 499 5
Fragments arranged by size:
499 117 81 5
PasI CC'CwG_GG
Cuts at: 0 126 702
Size: 126 576
PciI A'CATG_T
Cuts at: 0 77 194 693 702
Size: 77 117 499 9
Fragments arranged by size:
499 117 77 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 435 702
Size: 435 267
PfoI T'CCnGG_A
Cuts at: 0 238 278 702
Size: 238 40 424
Fragments arranged by size:
424 238 40
PpuMI rG'GwC_Cy
Cuts at: 0 29 633 702
Size: 29 604 69
Fragments arranged by size:
604 69 29
PshAI GACnn'nnGTC
Cuts at: 0 392 702
Size: 392 310
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 680 702
Size: 680 22
SacII CC_GC'GG
Cuts at: 0 65 702
Size: 65 637
SfoI GGC'GCC
Cuts at: 0 481 702
Size: 481 221
StyI C'CwwG_G
Cuts at: 0 434 702
Size: 434 268
TatI w'GTAC_w
Cuts at: 0 197 702
Size: 197 505
ZraI GAC'GTC
Cuts at: 0 356 597 702
Size: 356 241 105
Fragments arranged by size:
356 241 105
Enzymes that do cut:
AatII AcuI AfeI AflIII AlfI AloI ApaLI AvaI
BanI BbeI BbsI Bme1580I BmgBI BpmI Bpu10I BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BsrBI BsrFI BsrGI BssHII BstXI BtgI
ClaI EaeI EagI EarI Eco57MI EcoNI EcoO109I HaeII
HincII KasI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PpuMI PshAI
PsrI SacII SfoI StyI TatI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AleI
AlwNI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmrI BmtI BplI BpuEI BsaAI BsaBI
BsgI BsiWI BsmI BspHI BspMI BsrDI BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI CspCI
DraI DraIII DrdI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI