Restriction map for
pFN21AE3995
(Linear) (Six-base) MAPSORT of: KIEE3995.seq Check: 150 from: 1 to: 849
KIEE3995.orf
With 182 enzymes: SgfI *
March 22, 2012 17:06 ..
AflIII A'CryG_T
Cuts at: 0 716 849
Size: 716 133
AhdI GACnn_n'nnGTC
Cuts at: 0 613 849
Size: 613 236
AleI CACnn'nnGTG
Cuts at: 0 715 849
Size: 715 134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 766 798 849
Size: 766 32 51
Fragments arranged by size:
766 51 32
AlwNI CAG_nnn'CTG
Cuts at: 0 195 596 849
Size: 195 401 253
Fragments arranged by size:
401 253 195
ApaI G_GGCC'C
Cuts at: 0 444 849
Size: 444 405
ApoI r'AATT_y
Cuts at: 0 652 849
Size: 652 197
AvaI C'yCGr_G
Cuts at: 0 32 139 849
Size: 32 107 710
Fragments arranged by size:
710 107 32
BanI G'GyrC_C
Cuts at: 0 4 226 849
Size: 4 222 623
Fragments arranged by size:
623 222 4
BanII G_rGCy'C
Cuts at: 0 177 444 582 807 849
Size: 177 267 138 225 42
Fragments arranged by size:
267 225 177 138 42
BbeI G_GCGC'C
Cuts at: 0 8 230 849
Size: 8 222 619
Fragments arranged by size:
619 222 8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 120 154 309 343 849
Size: 120 34 155 34 506
Fragments arranged by size:
506 155 120 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 302 365 849
Size: 302 63 484
Fragments arranged by size:
484 302 63
BlpI GC'TnA_GC
Cuts at: 0 112 849
Size: 112 737
Bme1580I G_kGCm'C
Cuts at: 0 444 849
Size: 444 405
BmgBI CAC'GTC
Cuts at: 0 120 455 708 849
Size: 120 335 253 141
Fragments arranged by size:
335 253 141 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 849
Size: 804 45
Bpu10I CC'TnA_GC
Cuts at: 0 76 849
Size: 76 773
BsaI GGTCTCn'nnnn_
Cuts at: 0 18 849
Size: 18 831
BsaAI yAC'GTr
Cuts at: 0 717 849
Size: 717 132
BsaHI Gr'CG_yC
Cuts at: 0 5 227 629 740 849
Size: 5 222 402 111 109
Fragments arranged by size:
402 222 111 109 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 505 535 706 736 794 824 849
Size: 505 30 171 30 58 30 25
Fragments arranged by size:
505 171 58 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 523 526 601 849
Size: 523 3 75 248
Fragments arranged by size:
523 248 75 3
BseYI C'CCAG_C
Cuts at: 0 404 849
Size: 404 445
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 849
Size: 232 617
BsiEI CG_ry'CG
Cuts at: 0 237 849
Size: 237 612
Bsp1286I G_dGCh'C
Cuts at: 0 177 444 582 807 849
Size: 177 267 138 225 42
Fragments arranged by size:
267 225 177 138 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 483 849
Size: 483 366
BsrFI r'CCGG_y
Cuts at: 0 232 849
Size: 232 617
BsrGI T'GTAC_A
Cuts at: 0 602 849
Size: 602 247
BssHII G'CGCG_C
Cuts at: 0 239 314 316 849
Size: 239 75 2 533
Fragments arranged by size:
533 239 75 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 353 849
Size: 353 496
BstBI TT'CG_AA
Cuts at: 0 758 849
Size: 758 91
BtgI C'CryG_G
Cuts at: 0 222 849
Size: 222 627
BtsI GCAGTG_nn'
Cuts at: 0 688 849
Size: 688 161
EaeI y'GGCC_r
Cuts at: 0 186 234 357 523 792 849
Size: 186 48 123 166 269 57
Fragments arranged by size:
269 186 166 123 57 48
EagI C'GGCC_G
Cuts at: 0 234 849
Size: 234 615
EarI CTCTTCn'nnn_
Cuts at: 0 56 849
Size: 56 793
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 849
Size: 804 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 508 849
Size: 508 341
EcoO109I rG'GnC_Cy
Cuts at: 0 36 192 440 441 849
Size: 36 156 248 1 408
Fragments arranged by size:
408 248 156 36 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 513 849
Size: 513 336
HaeII r_GCGC'y
Cuts at: 0 8 70 147 230 849
Size: 8 62 77 83 619
Fragments arranged by size:
619 83 77 62 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 599 631 640 672 849
Size: 599 32 9 32 177
Fragments arranged by size:
599 177 32 32 9
KasI G'GCGC_C
Cuts at: 0 4 226 849
Size: 4 222 623
Fragments arranged by size:
623 222 4
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 749 849
Size: 642 107 100
Fragments arranged by size:
642 107 100
MscI TGG'CCA
Cuts at: 0 525 794 849
Size: 525 269 55
Fragments arranged by size:
525 269 55
MslI CAynn'nnrTG
Cuts at: 0 715 733 849
Size: 715 18 116
Fragments arranged by size:
715 116 18
MspA1I CmG'CkG
Cuts at: 0 263 476 593 849
Size: 263 213 117 256
Fragments arranged by size:
263 256 213 117
NarI GG'CG_CC
Cuts at: 0 5 227 849
Size: 5 222 622
Fragments arranged by size:
622 222 5
PasI CC'CwG_GG
Cuts at: 0 300 849
Size: 300 549
PmlI CAC'GTG
Cuts at: 0 717 849
Size: 717 132
PpuMI rG'GwC_Cy
Cuts at: 0 36 849
Size: 36 813
PspOMI G'GGCC_C
Cuts at: 0 440 849
Size: 440 409
PvuII CAG'CTG
Cuts at: 0 263 476 849
Size: 263 213 373
Fragments arranged by size:
373 263 213
SanDI GG'GwC_CC
Cuts at: 0 36 849
Size: 36 813
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 365 849
Size: 365 484
SfoI GGC'GCC
Cuts at: 0 6 228 849
Size: 6 222 621
Fragments arranged by size:
621 222 6
SmaI CCC'GGG
Cuts at: 0 34 849
Size: 34 815
StuI AGG'CCT
Cuts at: 0 291 368 849
Size: 291 77 481
Fragments arranged by size:
481 291 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 396 727 849
Size: 396 331 122
Fragments arranged by size:
396 331 122
TatI w'GTAC_w
Cuts at: 0 602 849
Size: 602 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 505 537 706 738 794 826 849
Size: 505 32 169 32 56 32 23
Fragments arranged by size:
505 169 56 32 32 32 23
XbaI T'CTAG_A
Cuts at: 0 624 645 849
Size: 624 21 204
Fragments arranged by size:
624 204 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 732 849
Size: 732 117
XmaI C'CCGG_G
Cuts at: 0 32 849
Size: 32 817
Enzymes that do cut:
AflIII AhdI AleI AloI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BcgI BglI BlpI Bme1580I BmgBI
BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI Bsp1286I BspMI BsrFI BsrGI BssHII BstAPI
BstBI BtgI BtsI EaeI EagI EarI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NarI PasI PmlI PpuMI PspOMI PvuII SanDI
SfiI SfoI SmaI StuI TaqII TatI TstI XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BfrBI BglII BmrI
BmtI BplI BpuEI BsaBI BsaWI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BssSI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StyI SwaI Tth111I XhoI
XmnI ZraI