(Linear) (Six-base) MAPSORT of: KIEE3992.seq  Check: 7348  from: 1  to: 1155



KIEE3992.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:05  ..

Acc65I G'GTAC_C

Cuts at:      0     350    1155 
   Size:        350     805
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     958    1155 
   Size:        192     766     197

  Fragments arranged by size: 

                 766     197     192
 
AlwNI CAG_nnn'CTG

Cuts at:      0     997    1155 
   Size:        997     158
 
ApoI r'AATT_y

Cuts at:      0     536     553     578     670     814    1155 
   Size:        536      17      25      92     144     341

  Fragments arranged by size: 

                 536     341     144      92      25      17
 
BanI G'GyrC_C

Cuts at:      0     350     660    1155 
   Size:        350     310     495

  Fragments arranged by size: 

                 495     350     310
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     198    1031    1155 
   Size:        198     833     124

  Fragments arranged by size: 

                 833     198     124
 
BbvCI CC'TCA_GC

Cuts at:      0    1046    1155 
   Size:       1046     109
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     615    1155 
   Size:        615     540
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1002    1155 
   Size:       1002     153
 
Bpu10I CC'TnA_GC

Cuts at:      0    1046    1155 
   Size:       1046     109
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218    1155 
   Size:        218     937
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     398    1155 
   Size:        398     757
 
BseYI C'CCAG_C

Cuts at:      0      73     368    1155 
   Size:         73     295     787

  Fragments arranged by size: 

                 787     295      73
 
BspHI T'CATG_A

Cuts at:      0     411    1155 
   Size:        411     744
 
BsrDI GCAATG_nn'

Cuts at:      0    1074    1155 
   Size:       1074      81
 
BsrFI r'CCGG_y

Cuts at:      0     777    1155 
   Size:        777     378
 
BsrGI T'GTAC_A

Cuts at:      0     521    1155 
   Size:        521     634
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     885    1155 
   Size:        885     270
 
BstBI TT'CG_AA

Cuts at:      0     495    1155 
   Size:        495     660
 
BstEII G'GTnAC_C

Cuts at:      0     856    1155 
   Size:        856     299
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      15    1155 
   Size:         15    1140
 
BtgI C'CryG_G

Cuts at:      0     964    1058    1155 
   Size:        964      94      97

  Fragments arranged by size: 

                 964      97      94
 
BtsI GCAGTG_nn'

Cuts at:      0     883    1155 
   Size:        883     272
 
DraI TTT'AAA

Cuts at:      0     932    1155 
   Size:        932     223
 
EaeI y'GGCC_r

Cuts at:      0     680    1155 
   Size:        680     475
 
EarI CTCTTCn'nnn_

Cuts at:      0     567    1155 
   Size:        567     588
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     808    1155 
   Size:        808     347
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     958    1002    1155 
   Size:        192     766      44     153

  Fragments arranged by size: 

                 766     192     153      44
 
EcoRI G'AATT_C

Cuts at:      0     536    1155 
   Size:        536     619
 
EcoRV GAT'ATC

Cuts at:      0     456    1155 
   Size:        456     699
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1104    1155 
   Size:       1104      51
 
KpnI G_GTAC'C

Cuts at:      0     354    1155 
   Size:        354     801
 
MscI TGG'CCA

Cuts at:      0     682    1155 
   Size:        682     473
 
MslI CAynn'nnrTG

Cuts at:      0     416     626    1155 
   Size:        416     210     529

  Fragments arranged by size: 

                 529     416     210
 
NaeI GCC'GGC

Cuts at:      0     779    1155 
   Size:        779     376
 
NdeI CA'TA_TG

Cuts at:      0     297    1155 
   Size:        297     858
 
NgoMIV G'CCGG_C

Cuts at:      0     777    1155 
   Size:        777     378
 
NspI r_CATG'y

Cuts at:      0     153    1045    1067    1155 
   Size:        153     892      22      88

  Fragments arranged by size: 

                 892     153      88      22
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     298    1155 
   Size:        298     857
 
PfoI T'CCnGG_A

Cuts at:      0      35     531    1155 
   Size:         35     496     624

  Fragments arranged by size: 

                 624     496      35
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     326     358    1155 
   Size:        326      32     797

  Fragments arranged by size: 

                 797     326      32
 
PstI C_TGCA'G

Cuts at:      0      52     890    1155 
   Size:         52     838     265

  Fragments arranged by size: 

                 838     265      52
 
SfcI C'TryA_G

Cuts at:      0      48     886    1155 
   Size:         48     838     269

  Fragments arranged by size: 

                 838     269      48
 
SmlI C'TyrA_G

Cuts at:      0     233    1155 
   Size:        233     922
 
SpeI A'CTAG_T

Cuts at:      0     339    1155 
   Size:        339     816
 
TatI w'GTAC_w

Cuts at:      0     333     521    1155 
   Size:        333     188     634

  Fragments arranged by size: 

                 634     333     188
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1043    1075    1155 
   Size:       1043      32      80

  Fragments arranged by size: 

                1043      80      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     654     707    1155 
   Size:        654      53     448

  Fragments arranged by size: 

                 654     448      53
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApoI     BanI     BbsI    BbvCI     BplI
     BpmI   Bpu10I    BpuEI    BseRI    BseYI    BspHI    BsrDI    BsrFI
    BsrGI   BstAPI    BstBI   BstEII    BstXI     BtgI     BtsI     DraI
     EaeI     EarI     EciI  Eco57MI    EcoRI    EcoRV     FalI     KpnI
     MscI     MslI     NaeI     NdeI   NgoMIV     NspI    PflMI     PfoI
     PsrI     PstI     SfcI     SmlI     SpeI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI   BssHII    BssSI    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MmeI   MspA1I     NarI     NcoI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI