(Linear) (Six-base) MAPSORT of: KIEE3991.seq  Check: 8106  from: 1  to: 885



KIEE3991.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:05  ..

AccI GT'mk_AC

Cuts at:      0     416     858     885 
   Size:        416     442      27

  Fragments arranged by size: 

                 442     416      27
 
AflIII A'CryG_T

Cuts at:      0     625     885 
   Size:        625     260
 
AhdI GACnn_n'nnGTC

Cuts at:      0      35     885 
   Size:         35     850
 
ApoI r'AATT_y

Cuts at:      0     158     509     873     885 
   Size:        158     351     364      12

  Fragments arranged by size: 

                 364     351     158      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     355     705     885 
   Size:        355     350     180

  Fragments arranged by size: 

                 355     350     180
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     226     885 
   Size:        226     659
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     306     885 
   Size:        306     579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     885 
   Size:        328     557
 
BsrDI GCAATG_nn'

Cuts at:      0     209     885 
   Size:        209     676
 
BstYI r'GATC_y

Cuts at:      0     753     885 
   Size:        753     132
 
BstZ17I GTA'TAC

Cuts at:      0     417     885 
   Size:        417     468
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     474     885 
   Size:        474     411
 
EcoRV GAT'ATC

Cuts at:      0     118     885 
   Size:        118     767
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      21      53     773     805     885 
   Size:         21      32     720      32      80

  Fragments arranged by size: 

                 720      80      32      32      21
 
MslI CAynn'nnrTG

Cuts at:      0     393     614     885 
   Size:        393     221     271

  Fragments arranged by size: 

                 393     271     221
 
MspA1I CmG'CkG

Cuts at:      0     687     885 
   Size:        687     198
 
NspI r_CATG'y

Cuts at:      0     629     885 
   Size:        629     256
 
PciI A'CATG_T

Cuts at:      0     625     885 
   Size:        625     260
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     436     885 
   Size:        436     449
 
PvuII CAG'CTG

Cuts at:      0     687     885 
   Size:        687     198
 
ScaI AGT'ACT

Cuts at:      0     557     885 
   Size:        557     328
 
SfcI C'TryA_G

Cuts at:      0      94     885 
   Size:         94     791
 
SmlI C'TyrA_G

Cuts at:      0     343     885 
   Size:        343     542
 
SspI AAT'ATT

Cuts at:      0     833     885 
   Size:        833      52
 
StyI C'CwwG_G

Cuts at:      0     133     885 
   Size:        133     752
 
TatI w'GTAC_w

Cuts at:      0     293     555     885 
   Size:        293     262     330

  Fragments arranged by size: 

                 330     293     262
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     627     885 
   Size:        627     258
 
XmnI GAAnn'nnTTC

Cuts at:      0     338     834     885 
   Size:        338     496      51

  Fragments arranged by size: 

                 496     338      51
 

 Enzymes that do cut:

     AccI   AflIII     AhdI     ApoI     BbsI    BciVI     BmrI    BpuEI
    BsrDI    BstYI  BstZ17I     DrdI    EcoRV    Hin4I     MslI   MspA1I
     NspI     PciI    PflMI    PvuII     ScaI     SfcI     SmlI     SspI
     StyI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI