Restriction map for
pFN21AE3988
(Linear) (Six-base) MAPSORT of: KIEE3988.seq Check: 2786 from: 1 to: 984
KIEE3988.orf
With 182 enzymes: SgfI *
March 22, 2012 17:04 ..
AccI GT'mk_AC
Cuts at: 0 407 984
Size: 407 577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 984
Size: 345 639
ApoI r'AATT_y
Cuts at: 0 772 984
Size: 772 212
BanII G_rGCy'C
Cuts at: 0 728 984
Size: 728 256
BbsI GAAGACnn'nnnn_
Cuts at: 0 98 984
Size: 98 886
BclI T'GATC_A
Cuts at: 0 854 984
Size: 854 130
BfrBI ATG'CAT
Cuts at: 0 445 984
Size: 445 539
BglII A'GATC_T
Cuts at: 0 596 984
Size: 596 388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 84 618 984
Size: 84 534 366
Fragments arranged by size:
534 366 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 984
Size: 61 923
BsaAI yAC'GTr
Cuts at: 0 156 984
Size: 156 828
BsaBI GATnn'nnATC
Cuts at: 0 169 984
Size: 169 815
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 984
Size: 106 30 848
Fragments arranged by size:
848 106 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 984
Size: 701 283
Bsp1286I G_dGCh'C
Cuts at: 0 728 984
Size: 728 256
BspHI T'CATG_A
Cuts at: 0 768 984
Size: 768 216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 984
Size: 113 871
BsrDI GCAATG_nn'
Cuts at: 0 191 984
Size: 191 793
BsrFI r'CCGG_y
Cuts at: 0 33 644 984
Size: 33 611 340
Fragments arranged by size:
611 340 33
BsrGI T'GTAC_A
Cuts at: 0 122 984
Size: 122 862
BstEII G'GTnAC_C
Cuts at: 0 249 984
Size: 249 735
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 984
Size: 156 828
BstYI r'GATC_y
Cuts at: 0 596 984
Size: 596 388
BtgI C'CryG_G
Cuts at: 0 5 527 984
Size: 5 522 457
Fragments arranged by size:
522 457 5
EaeI y'GGCC_r
Cuts at: 0 2 231 984
Size: 2 229 753
Fragments arranged by size:
753 229 2
EarI CTCTTCn'nnn_
Cuts at: 0 364 841 984
Size: 364 477 143
Fragments arranged by size:
477 364 143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 345 984
Size: 61 284 639
Fragments arranged by size:
639 284 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 109 984
Size: 109 875
EcoO109I rG'GnC_Cy
Cuts at: 0 45 984
Size: 45 939
EcoRI G'AATT_C
Cuts at: 0 772 984
Size: 772 212
HaeII r_GCGC'y
Cuts at: 0 34 984
Size: 34 950
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 846 878 984
Size: 846 32 106
Fragments arranged by size:
846 106 32
HindIII A'AGCT_T
Cuts at: 0 79 984
Size: 79 905
MslI CAynn'nnrTG
Cuts at: 0 154 984
Size: 154 830
MspA1I CmG'CkG
Cuts at: 0 7 984
Size: 7 977
NcoI C'CATG_G
Cuts at: 0 527 984
Size: 527 457
NsiI A_TGCA'T
Cuts at: 0 447 984
Size: 447 537
PasI CC'CwG_GG
Cuts at: 0 866 984
Size: 866 118
PfoI T'CCnGG_A
Cuts at: 0 899 984
Size: 899 85
PmlI CAC'GTG
Cuts at: 0 156 984
Size: 156 828
PstI C_TGCA'G
Cuts at: 0 90 110 984
Size: 90 20 874
Fragments arranged by size:
874 90 20
SacII CC_GC'GG
Cuts at: 0 8 984
Size: 8 976
SfcI C'TryA_G
Cuts at: 0 86 106 623 633 984
Size: 86 20 517 10 351
Fragments arranged by size:
517 351 86 20 10
SgrAI Cr'CCGG_yG
Cuts at: 0 33 984
Size: 33 951
StyI C'CwwG_G
Cuts at: 0 527 984
Size: 527 457
TatI w'GTAC_w
Cuts at: 0 122 984
Size: 122 862
Enzymes that do cut:
AccI AcuI ApoI BanII BbsI BclI BfrBI BglII
BplI BpmI BsaAI BsaBI BsaXI BsgI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BstEII BstXI BstYI BtgI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HindIII MslI MspA1I NcoI NsiI PasI PfoI PmlI
PstI SacII SfcI SgrAI StyI TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI
BmtI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI PacI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI