Restriction map for
pFN21AE3987
(Linear) (Six-base) MAPSORT of: KIEE3987.seq Check: 7153 from: 1 to: 978
KIEE3987.orf
With 182 enzymes: SgfI *
March 22, 2012 17:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 512 978
Size: 512 466
AccI GT'mk_AC
Cuts at: 0 140 978
Size: 140 838
Acc65I G'GTAC_C
Cuts at: 0 553 978
Size: 553 425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 978
Size: 331 647
AflIII A'CryG_T
Cuts at: 0 135 978
Size: 135 843
AgeI A'CCGG_T
Cuts at: 0 119 978
Size: 119 859
AhdI GACnn_n'nnGTC
Cuts at: 0 169 978
Size: 169 809
AlwNI CAG_nnn'CTG
Cuts at: 0 89 513 873 978
Size: 89 424 360 105
Fragments arranged by size:
424 360 105 89
AvaI C'yCGr_G
Cuts at: 0 37 978
Size: 37 941
BamHI G'GATC_C
Cuts at: 0 71 978
Size: 71 907
BanI G'GyrC_C
Cuts at: 0 179 348 553 978
Size: 179 169 205 425
Fragments arranged by size:
425 205 179 169
BanII G_rGCy'C
Cuts at: 0 288 602 978
Size: 288 314 376
Fragments arranged by size:
376 314 288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 169 203 978
Size: 169 34 775
Fragments arranged by size:
775 169 34
BfrBI ATG'CAT
Cuts at: 0 464 978
Size: 464 514
BglII A'GATC_T
Cuts at: 0 41 404 978
Size: 41 363 574
Fragments arranged by size:
574 363 41
BlpI GC'TnA_GC
Cuts at: 0 601 978
Size: 601 377
Bme1580I G_kGCm'C
Cuts at: 0 304 353 978
Size: 304 49 625
Fragments arranged by size:
625 304 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 255 978
Size: 255 723
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 63 978
Size: 63 915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 406 763 781 978
Size: 66 340 357 18 197
Fragments arranged by size:
357 340 197 66 18
Bpu10I CC'TnA_GC
Cuts at: 0 340 978
Size: 340 638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 640 880 978
Size: 396 244 240 98
Fragments arranged by size:
396 244 240 98
BsaBI GATnn'nnATC
Cuts at: 0 76 978
Size: 76 902
BsaWI w'CCGG_w
Cuts at: 0 68 119 978
Size: 68 51 859
Fragments arranged by size:
859 68 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 754 784 978
Size: 754 30 194
Fragments arranged by size:
754 194 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 816 978
Size: 816 162
BseYI C'CCAG_C
Cuts at: 0 367 546 627 873 978
Size: 367 179 81 246 105
Fragments arranged by size:
367 246 179 105 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 618 978
Size: 376 242 360
Fragments arranged by size:
376 360 242
BsiEI CG_ry'CG
Cuts at: 0 120 978
Size: 120 858
BsiHKAI G_wGCw'C
Cuts at: 0 503 602 978
Size: 503 99 376
Fragments arranged by size:
503 376 99
BsmI GAATG_Cn'
Cuts at: 0 466 755 978
Size: 466 289 223
Fragments arranged by size:
466 289 223
Bsp1286I G_dGCh'C
Cuts at: 0 288 304 353 503 602 978
Size: 288 16 49 150 99 376
Fragments arranged by size:
376 288 150 99 49 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 512 978
Size: 512 466
BsrFI r'CCGG_y
Cuts at: 0 119 753 978
Size: 119 634 225
Fragments arranged by size:
634 225 119
BstBI TT'CG_AA
Cuts at: 0 189 978
Size: 189 789
BstYI r'GATC_y
Cuts at: 0 41 71 404 695 902 978
Size: 41 30 333 291 207 76
Fragments arranged by size:
333 291 207 76 41 30
BtgI C'CryG_G
Cuts at: 0 410 978
Size: 410 568
EaeI y'GGCC_r
Cuts at: 0 332 662 978
Size: 332 330 316
Fragments arranged by size:
332 330 316
EarI CTCTTCn'nnn_
Cuts at: 0 97 978
Size: 97 881
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 910 978
Size: 910 68
EcoICRI GAG'CTC
Cuts at: 0 600 978
Size: 600 378
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 331 406 763 781 978
Size: 66 265 75 357 18 197
Fragments arranged by size:
357 265 197 75 66 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 910 978
Size: 910 68
EcoO109I rG'GnC_Cy
Cuts at: 0 255 380 435 510 714 781 978
Size: 255 125 55 75 204 67 197
Fragments arranged by size:
255 204 197 125 75 67 55
FspI TGC'GCA
Cuts at: 0 691 978
Size: 691 287
HaeII r_GCGC'y
Cuts at: 0 318 978
Size: 318 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 155 187 978
Size: 155 32 791
Fragments arranged by size:
791 155 32
HincII GTy'rAC
Cuts at: 0 141 978
Size: 141 837
KpnI G_GTAC'C
Cuts at: 0 557 978
Size: 557 421
MscI TGG'CCA
Cuts at: 0 664 978
Size: 664 314
MspA1I CmG'CkG
Cuts at: 0 98 398 978
Size: 98 300 580
Fragments arranged by size:
580 300 98
NaeI GCC'GGC
Cuts at: 0 755 978
Size: 755 223
NcoI C'CATG_G
Cuts at: 0 410 978
Size: 410 568
NgoMIV G'CCGG_C
Cuts at: 0 753 978
Size: 753 225
NsiI A_TGCA'T
Cuts at: 0 466 978
Size: 466 512
NspI r_CATG'y
Cuts at: 0 139 331 978
Size: 139 192 647
Fragments arranged by size:
647 192 139
PasI CC'CwG_GG
Cuts at: 0 288 978
Size: 288 690
PciI A'CATG_T
Cuts at: 0 135 978
Size: 135 843
PflMI CCAn_nnn'nTGG
Cuts at: 0 416 930 978
Size: 416 514 48
Fragments arranged by size:
514 416 48
PpuMI rG'GwC_Cy
Cuts at: 0 380 435 781 978
Size: 380 55 346 197
Fragments arranged by size:
380 346 197 55
PshAI GACnn'nnGTC
Cuts at: 0 10 978
Size: 10 968
PstI C_TGCA'G
Cuts at: 0 509 596 614 779 869 978
Size: 509 87 18 165 90 109
Fragments arranged by size:
509 165 109 90 87 18
PvuII CAG'CTG
Cuts at: 0 398 978
Size: 398 580
SacI G_AGCT'C
Cuts at: 0 602 978
Size: 602 376
SalI G'TCGA_C
Cuts at: 0 139 978
Size: 139 839
SanDI GG'GwC_CC
Cuts at: 0 781 978
Size: 781 197
SbfI CC_TGCA'GG
Cuts at: 0 509 596 779 978
Size: 509 87 183 199
Fragments arranged by size:
509 199 183 87
SfcI C'TryA_G
Cuts at: 0 505 592 610 775 865 978
Size: 505 87 18 165 90 113
Fragments arranged by size:
505 165 113 90 87 18
SmlI C'TyrA_G
Cuts at: 0 37 375 619 859 978
Size: 37 338 244 240 119
Fragments arranged by size:
338 244 240 119 37
StyI C'CwwG_G
Cuts at: 0 410 978
Size: 410 568
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 18 217 538 583 978
Size: 18 199 321 45 395
Fragments arranged by size:
395 321 199 45 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 106 138 754 786 978
Size: 106 32 616 32 192
Fragments arranged by size:
616 192 106 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 28 478 978
Size: 28 450 500
Fragments arranged by size:
500 450 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 960 978
Size: 960 18
XhoI C'TCGA_G
Cuts at: 0 37 978
Size: 37 941
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AhdI AlwNI
AvaI BamHI BanI BanII BcgI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrFI BstBI BstYI BtgI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII
KpnI MscI MspA1I NaeI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PpuMI PshAI PstI PvuII SacI
SalI SanDI SbfI SfcI SmlI StyI TaqII TstI
Tth111I XcmI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflII AleI AlfI AloI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BbvCI BciVI BclI BglI BmgBI BmtI BsaI
BsaAI BsaHI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI
MfeI MluI MmeI MslI NarI NdeI NheI NotI
NruI PacI PfoI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI XbaI XmaI XmnI
ZraI