Restriction map for
pFN21AE3984
(Linear) (Six-base) MAPSORT of: KIEE3984.seq Check: 9132 from: 1 to: 612
KIEE3984.orf
With 182 enzymes: SgfI *
March 22, 2012 17:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 90 612
Size: 90 522
AccI GT'mk_AC
Cuts at: 0 184 612
Size: 184 428
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 364 612
Size: 317 47 248
Fragments arranged by size:
317 248 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 81 612
Size: 81 531
AlwNI CAG_nnn'CTG
Cuts at: 0 127 612
Size: 127 485
AseI AT'TA_AT
Cuts at: 0 572 612
Size: 572 40
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 387 612
Size: 387 225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 612
Size: 297 315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 612
Size: 284 328
BsaAI yAC'GTr
Cuts at: 0 496 612
Size: 496 116
BsaWI w'CCGG_w
Cuts at: 0 11 266 612
Size: 11 255 346
Fragments arranged by size:
346 255 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 352 612
Size: 68 284 260
Fragments arranged by size:
284 260 68
BsiHKAI G_wGCw'C
Cuts at: 0 60 612
Size: 60 552
BsmI GAATG_Cn'
Cuts at: 0 228 612
Size: 228 384
Bsp1286I G_dGCh'C
Cuts at: 0 60 612
Size: 60 552
BspEI T'CCGG_A
Cuts at: 0 11 266 612
Size: 11 255 346
Fragments arranged by size:
346 255 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 90 612
Size: 90 522
BsrGI T'GTAC_A
Cuts at: 0 32 612
Size: 32 580
BstYI r'GATC_y
Cuts at: 0 295 612
Size: 295 317
BtsI GCAGTG_nn'
Cuts at: 0 165 215 612
Size: 165 50 397
Fragments arranged by size:
397 165 50
EaeI y'GGCC_r
Cuts at: 0 419 612
Size: 419 193
EarI CTCTTCn'nnn_
Cuts at: 0 336 383 612
Size: 336 47 229
Fragments arranged by size:
336 229 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 317 364 612
Size: 297 20 47 248
Fragments arranged by size:
297 248 47 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 544 576 612
Size: 313 32 199 32 36
Fragments arranged by size:
313 199 36 32 32
HincII GTy'rAC
Cuts at: 0 185 612
Size: 185 427
MscI TGG'CCA
Cuts at: 0 421 612
Size: 421 191
MslI CAynn'nnrTG
Cuts at: 0 393 612
Size: 393 219
MspA1I CmG'CkG
Cuts at: 0 567 612
Size: 567 45
PmlI CAC'GTG
Cuts at: 0 496 612
Size: 496 116
PstI C_TGCA'G
Cuts at: 0 160 612
Size: 160 452
PvuII CAG'CTG
Cuts at: 0 567 612
Size: 567 45
SalI G'TCGA_C
Cuts at: 0 183 612
Size: 183 429
SapI GCTCTTCn'nnn_
Cuts at: 0 383 612
Size: 383 229
SexAI A'CCwGG_T
Cuts at: 0 101 612
Size: 101 511
SfcI C'TryA_G
Cuts at: 0 156 514 612
Size: 156 358 98
Fragments arranged by size:
358 156 98
SmlI C'TyrA_G
Cuts at: 0 299 612
Size: 299 313
SspI AAT'ATT
Cuts at: 0 311 416 472 612
Size: 311 105 56 140
Fragments arranged by size:
311 140 105 56
StuI AGG'CCT
Cuts at: 0 116 612
Size: 116 496
StyI C'CwwG_G
Cuts at: 0 291 612
Size: 291 321
TatI w'GTAC_w
Cuts at: 0 32 453 612
Size: 32 421 159
Fragments arranged by size:
421 159 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 612
Size: 31 32 549
Fragments arranged by size:
549 32 31
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI AseI BplI BpmI
BpuEI BsaAI BsaWI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrGI BstYI BtsI EaeI EarI Eco57MI Hin4I
HincII MscI MslI MspA1I PmlI PstI PvuII SalI
SapI SexAI SfcI SmlI SspI StuI StyI TatI
TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI Bpu10I BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsiEI BsiWI BsmBI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI