Restriction map for
pFN21AE3980
(Linear) (Six-base) MAPSORT of: KIEE3980.seq Check: 6045 from: 1 to: 1587
KIEE3980.orf
With 182 enzymes: SgfI *
March 22, 2012 17:03 ..
AccI GT'mk_AC
Cuts at: 0 1181 1429 1587
Size: 1181 248 158
Fragments arranged by size:
1181 248 158
AflIII A'CryG_T
Cuts at: 0 1280 1587
Size: 1280 307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 86 1120 1587
Size: 86 1034 467
Fragments arranged by size:
1034 467 86
AlwNI CAG_nnn'CTG
Cuts at: 0 1092 1195 1587
Size: 1092 103 392
Fragments arranged by size:
1092 392 103
AvaI C'yCGr_G
Cuts at: 0 1504 1587
Size: 1504 83
BanI G'GyrC_C
Cuts at: 0 30 1587
Size: 30 1557
BanII G_rGCy'C
Cuts at: 0 508 742 769 833 1318 1587
Size: 508 234 27 64 485 269
Fragments arranged by size:
508 485 269 234 64 27
BbeI G_GCGC'C
Cuts at: 0 34 1587
Size: 34 1553
BbsI GAAGACnn'nnnn_
Cuts at: 0 689 1587
Size: 689 898
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 513 547 1587
Size: 513 34 1040
Fragments arranged by size:
1040 513 34
BclI T'GATC_A
Cuts at: 0 131 1587
Size: 131 1456
BglI GCCn_nnn'nGGC
Cuts at: 0 394 1587
Size: 394 1193
Bme1580I G_kGCm'C
Cuts at: 0 338 937 1364 1587
Size: 338 599 427 223
Fragments arranged by size:
599 427 338 223
BmgBI CAC'GTC
Cuts at: 0 1437 1587
Size: 1437 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 585 640 1587
Size: 585 55 947
Fragments arranged by size:
947 585 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 598 1155 1587
Size: 373 225 557 432
Fragments arranged by size:
557 432 373 225
BsaI GGTCTCn'nnnn_
Cuts at: 0 197 574 1518 1587
Size: 197 377 944 69
Fragments arranged by size:
944 377 197 69
BsaBI GATnn'nnATC
Cuts at: 0 136 1587
Size: 136 1451
BsaHI Gr'CG_yC
Cuts at: 0 31 281 1587
Size: 31 250 1306
Fragments arranged by size:
1306 250 31
BsaWI w'CCGG_w
Cuts at: 0 186 875 1587
Size: 186 689 712
Fragments arranged by size:
712 689 186
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 790 820 1587
Size: 790 30 767
Fragments arranged by size:
790 767 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 688 732 735 992 1429 1587
Size: 370 318 44 3 257 437 158
Fragments arranged by size:
437 370 318 257 158 44 3
BseYI C'CCAG_C
Cuts at: 0 271 375 458 1125 1219 1333 1528 1587
Size: 271 104 83 667 94 114 195 59
Fragments arranged by size:
667 271 195 114 104 94 83 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1587
Size: 967 620
BsiEI CG_ry'CG
Cuts at: 0 261 1587
Size: 261 1326
BsiHKAI G_wGCw'C
Cuts at: 0 508 833 1587
Size: 508 325 754
Fragments arranged by size:
754 508 325
BsmI GAATG_Cn'
Cuts at: 0 1577 1587
Size: 1577 10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 540 793 1377 1396 1587
Size: 540 253 584 19 191
Fragments arranged by size:
584 540 253 191 19
Bsp1286I G_dGCh'C
Cuts at: 0 338 508 742 769 833 937 1318 1364
Size: 338 170 234 27 64 104 381 46
Cuts at: 1364 1587
Size: 223
Fragments arranged by size:
381 338 234 223 170 104 64 46
27
BspEI T'CCGG_A
Cuts at: 0 186 875 1587
Size: 186 689 712
Fragments arranged by size:
712 689 186
BspHI T'CATG_A
Cuts at: 0 884 1587
Size: 884 703
BsrBI CCG'CTC
Cuts at: 0 123 1587
Size: 123 1464
BsrDI GCAATG_nn'
Cuts at: 0 1346 1587
Size: 1346 241
BsrFI r'CCGG_y
Cuts at: 0 91 212 266 324 513 864 1587
Size: 91 121 54 58 189 351 723
Fragments arranged by size:
723 351 189 121 91 58 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1011 1296 1587
Size: 1011 285 291
Fragments arranged by size:
1011 291 285
BstYI r'GATC_y
Cuts at: 0 359 1088 1587
Size: 359 729 499
Fragments arranged by size:
729 499 359
BstZ17I GTA'TAC
Cuts at: 0 1430 1587
Size: 1430 157
Bsu36I CC'TnA_GG
Cuts at: 0 238 1465 1587
Size: 238 1227 122
Fragments arranged by size:
1227 238 122
BtgI C'CryG_G
Cuts at: 0 45 1262 1587
Size: 45 1217 325
Fragments arranged by size:
1217 325 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 510 1314 1587
Size: 510 804 273
Fragments arranged by size:
804 510 273
BtsI GCAGTG_nn'
Cuts at: 0 1253 1587
Size: 1253 334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1423 1458 1587
Size: 1423 35 129
Fragments arranged by size:
1423 129 35
EaeI y'GGCC_r
Cuts at: 0 210 515 984 1013 1587
Size: 210 305 469 29 574
Fragments arranged by size:
574 469 305 210 29
EarI CTCTTCn'nnn_
Cuts at: 0 665 1153 1171 1587
Size: 665 488 18 416
Fragments arranged by size:
665 488 416 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 837 1021 1587
Size: 837 184 566
Fragments arranged by size:
837 566 184
EcoICRI GAG'CTC
Cuts at: 0 506 831 1587
Size: 506 325 756
Fragments arranged by size:
756 506 325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 598 1155 1587
Size: 373 225 557 432
Fragments arranged by size:
557 432 373 225
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1116 1587
Size: 1116 471
EcoO109I rG'GnC_Cy
Cuts at: 0 387 1338 1587
Size: 387 951 249
Fragments arranged by size:
951 387 249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 885 1515 1587
Size: 885 630 72
Fragments arranged by size:
885 630 72
FseI GG_CCGG'CC
Cuts at: 0 517 1587
Size: 517 1070
FspI TGC'GCA
Cuts at: 0 169 1587
Size: 169 1418
HaeII r_GCGC'y
Cuts at: 0 34 474 1587
Size: 34 440 1113
Fragments arranged by size:
1113 440 34
HincII GTy'rAC
Cuts at: 0 330 1587
Size: 330 1257
KasI G'GCGC_C
Cuts at: 0 30 1587
Size: 30 1557
MfeI C'AATT_G
Cuts at: 0 163 1587
Size: 163 1424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1400 1587
Size: 1400 187
MscI TGG'CCA
Cuts at: 0 1015 1587
Size: 1015 572
MslI CAynn'nnrTG
Cuts at: 0 1294 1587
Size: 1294 293
MspA1I CmG'CkG
Cuts at: 0 375 450 1587
Size: 375 75 1137
Fragments arranged by size:
1137 375 75
NaeI GCC'GGC
Cuts at: 0 93 214 515 866 1587
Size: 93 121 301 351 721
Fragments arranged by size:
721 351 301 121 93
NarI GG'CG_CC
Cuts at: 0 31 1587
Size: 31 1556
NgoMIV G'CCGG_C
Cuts at: 0 91 212 513 864 1587
Size: 91 121 301 351 723
Fragments arranged by size:
723 351 301 121 91
NspI r_CATG'y
Cuts at: 0 1284 1587
Size: 1284 303
PasI CC'CwG_GG
Cuts at: 0 718 930 1587
Size: 718 212 657
Fragments arranged by size:
718 657 212
PciI A'CATG_T
Cuts at: 0 1280 1587
Size: 1280 307
PflMI CCAn_nnn'nTGG
Cuts at: 0 973 1587
Size: 973 614
PfoI T'CCnGG_A
Cuts at: 0 83 350 401 1226 1587
Size: 83 267 51 825 361
Fragments arranged by size:
825 361 267 83 51
PpuMI rG'GwC_Cy
Cuts at: 0 1338 1587
Size: 1338 249
PstI C_TGCA'G
Cuts at: 0 1088 1587
Size: 1088 499
PvuII CAG'CTG
Cuts at: 0 375 1587
Size: 375 1212
SacI G_AGCT'C
Cuts at: 0 508 833 1587
Size: 508 325 754
Fragments arranged by size:
754 508 325
ScaI AGT'ACT
Cuts at: 0 250 756 1587
Size: 250 506 831
Fragments arranged by size:
831 506 250
SfcI C'TryA_G
Cuts at: 0 111 1084 1587
Size: 111 973 503
Fragments arranged by size:
973 503 111
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 394 1587
Size: 394 1193
SfoI GGC'GCC
Cuts at: 0 32 1587
Size: 32 1555
StuI AGG'CCT
Cuts at: 0 1111 1587
Size: 1111 476
StyI C'CwwG_G
Cuts at: 0 1076 1587
Size: 1076 511
TatI w'GTAC_w
Cuts at: 0 248 754 1587
Size: 248 506 833
Fragments arranged by size:
833 506 248
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 790 822 1295 1327 1587
Size: 790 32 473 32 260
Fragments arranged by size:
790 473 260 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1122 1441 1587
Size: 1122 319 146
Fragments arranged by size:
1122 319 146
Enzymes that do cut:
AccI AflIII AlfI AlwNI AvaI BanI BanII BbeI
BbsI BcgI BclI BglI Bme1580I BmgBI BmrI BpmI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI
BsrDI BsrFI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI FspI HaeII HincII KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PpuMI PstI PvuII SacI
ScaI SfcI SfiI SfoI StuI StyI TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI BglII
BlpI BmtI BplI Bpu10I BpuEI BsaAI BsiWI BspMI
BsrGI BssHII BssSI BstAPI BstBI BstEII ClaI DraI
DraIII DrdI EagI EcoRI EcoRV FspAI Hin4I HindIII
HpaI KpnI MluI NcoI NdeI NheI NotI NruI
NsiI PacI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI
XmaI XmnI ZraI