Restriction map for
pFN21AE3979
(Linear) (Six-base) MAPSORT of: KIEE3979.seq Check: 1116 from: 1 to: 1308
KIEE3979.orf
With 182 enzymes: SgfI *
March 22, 2012 17:02 ..
AclI AA'CG_TT
Cuts at: 0 951 1308
Size: 951 357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 910 1308
Size: 100 810 398
Fragments arranged by size:
810 398 100
AhdI GACnn_n'nnGTC
Cuts at: 0 357 1308
Size: 357 951
AlwNI CAG_nnn'CTG
Cuts at: 0 455 516 1153 1308
Size: 455 61 637 155
Fragments arranged by size:
637 455 155 61
ApaLI G'TGCA_C
Cuts at: 0 1013 1308
Size: 1013 295
ApoI r'AATT_y
Cuts at: 0 1160 1297 1308
Size: 1160 137 11
Fragments arranged by size:
1160 137 11
AscI GG'CGCG_CC
Cuts at: 0 418 1308
Size: 418 890
AvaI C'yCGr_G
Cuts at: 0 68 142 322 1308
Size: 68 74 180 986
Fragments arranged by size:
986 180 74 68
BanI G'GyrC_C
Cuts at: 0 49 1308
Size: 49 1259
BanII G_rGCy'C
Cuts at: 0 275 491 659 1256 1308
Size: 275 216 168 597 52
Fragments arranged by size:
597 275 216 168 52
BbeI G_GCGC'C
Cuts at: 0 53 1308
Size: 53 1255
BbsI GAAGACnn'nnnn_
Cuts at: 0 916 975 1308
Size: 916 59 333
Fragments arranged by size:
916 333 59
BciVI GTATCCnnnnn_n'
Cuts at: 0 1148 1308
Size: 1148 160
BglI GCCn_nnn'nGGC
Cuts at: 0 311 439 585 1308
Size: 311 128 146 723
Fragments arranged by size:
723 311 146 128
BglII A'GATC_T
Cuts at: 0 1264 1308
Size: 1264 44
Bme1580I G_kGCm'C
Cuts at: 0 1017 1308
Size: 1017 291
BmgBI CAC'GTC
Cuts at: 0 138 494 1308
Size: 138 356 814
Fragments arranged by size:
814 356 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 309 1308
Size: 309 999
BmtI G_CTAG'C
Cuts at: 0 524 1308
Size: 524 784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 432 481 826 872 1308
Size: 328 104 49 345 46 436
Fragments arranged by size:
436 345 328 104 49 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 882 1240 1308
Size: 882 358 68
Fragments arranged by size:
882 358 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 747 1308
Size: 747 561
BsaAI yAC'GTr
Cuts at: 0 228 1308
Size: 228 1080
BsaBI GATnn'nnATC
Cuts at: 0 1136 1308
Size: 1136 172
BsaHI Gr'CG_yC
Cuts at: 0 50 1308
Size: 50 1258
BsaWI w'CCGG_w
Cuts at: 0 623 1308
Size: 623 685
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 478 508 1163 1193 1308
Size: 478 30 655 30 115
Fragments arranged by size:
655 478 115 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 499 1184 1308
Size: 499 685 124
Fragments arranged by size:
685 499 124
BseYI C'CCAG_C
Cuts at: 0 402 1308
Size: 402 906
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1258 1308
Size: 97 1161 50
Fragments arranged by size:
1161 97 50
BsiEI CG_ry'CG
Cuts at: 0 165 192 354 386 1308
Size: 165 27 162 32 922
Fragments arranged by size:
922 165 162 32 27
BsiHKAI G_wGCw'C
Cuts at: 0 143 275 1017 1256 1308
Size: 143 132 742 239 52
Fragments arranged by size:
742 239 143 132 52
BsmI GAATG_Cn'
Cuts at: 0 1076 1308
Size: 1076 232
Bsp1286I G_dGCh'C
Cuts at: 0 143 275 491 659 1017 1256 1308
Size: 143 132 216 168 358 239 52
Fragments arranged by size:
358 239 216 168 143 132 52
BspHI T'CATG_A
Cuts at: 0 1248 1308
Size: 1248 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 620 1308
Size: 620 688
BsrBI CCG'CTC
Cuts at: 0 125 1308
Size: 125 1183
BsrFI r'CCGG_y
Cuts at: 0 107 191 640 1308
Size: 107 84 449 668
Fragments arranged by size:
668 449 107 84
BssHII G'CGCG_C
Cuts at: 0 314 418 1308
Size: 314 104 890
Fragments arranged by size:
890 314 104
BstXI CCAn_nnnn'nTGG
Cuts at: 0 850 1308
Size: 850 458
BstYI r'GATC_y
Cuts at: 0 512 1264 1308
Size: 512 752 44
Fragments arranged by size:
752 512 44
BtgI C'CryG_G
Cuts at: 0 237 259 551 998 1308
Size: 237 22 292 447 310
Fragments arranged by size:
447 310 292 237 22
BtsI GCAGTG_nn'
Cuts at: 0 695 1246 1308
Size: 695 551 62
Fragments arranged by size:
695 551 62
EaeI y'GGCC_r
Cuts at: 0 234 383 503 1308
Size: 234 149 120 805
Fragments arranged by size:
805 234 149 120
EagI C'GGCC_G
Cuts at: 0 383 1308
Size: 383 925
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 489 627 1308
Size: 489 138 681
Fragments arranged by size:
681 489 138
EcoICRI GAG'CTC
Cuts at: 0 273 1254 1308
Size: 273 981 54
Fragments arranged by size:
981 273 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 328 432 481 826 872 910 1308
Size: 100 228 104 49 345 46 38 398
Fragments arranged by size:
398 345 228 104 100 49 46 38
EcoO109I rG'GnC_Cy
Cuts at: 0 303 1308
Size: 303 1005
EcoRI G'AATT_C
Cuts at: 0 1160 1297 1308
Size: 1160 137 11
Fragments arranged by size:
1160 137 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 267 1308
Size: 267 1041
HaeII r_GCGC'y
Cuts at: 0 53 342 456 1308
Size: 53 289 114 852
Fragments arranged by size:
852 289 114 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 343 375 478 510 1308
Size: 181 32 130 32 103 32 798
Fragments arranged by size:
798 181 130 103 32 32 32
HindIII A'AGCT_T
Cuts at: 0 901 1308
Size: 901 407
KasI G'GCGC_C
Cuts at: 0 49 1308
Size: 49 1259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1082 1308
Size: 386 696 226
Fragments arranged by size:
696 386 226
MslI CAynn'nnrTG
Cuts at: 0 556 802 1308
Size: 556 246 506
Fragments arranged by size:
556 506 246
MspA1I CmG'CkG
Cuts at: 0 17 93 261 1308
Size: 17 76 168 1047
Fragments arranged by size:
1047 168 76 17
NaeI GCC'GGC
Cuts at: 0 109 642 1308
Size: 109 533 666
Fragments arranged by size:
666 533 109
NarI GG'CG_CC
Cuts at: 0 50 1308
Size: 50 1258
NcoI C'CATG_G
Cuts at: 0 551 998 1308
Size: 551 447 310
Fragments arranged by size:
551 447 310
NgoMIV G'CCGG_C
Cuts at: 0 107 640 1308
Size: 107 533 668
Fragments arranged by size:
668 533 107
NheI G'CTAG_C
Cuts at: 0 520 1308
Size: 520 788
NspI r_CATG'y
Cuts at: 0 360 471 1308
Size: 360 111 837
Fragments arranged by size:
837 360 111
PasI CC'CwG_GG
Cuts at: 0 646 1308
Size: 646 662
PflMI CCAn_nnn'nTGG
Cuts at: 0 455 1308
Size: 455 853
PfoI T'CCnGG_A
Cuts at: 0 661 1308
Size: 661 647
PspXI vC'TCGA_Gb
Cuts at: 0 142 322 1308
Size: 142 180 986
Fragments arranged by size:
986 180 142
SacI G_AGCT'C
Cuts at: 0 275 1256 1308
Size: 275 981 52
Fragments arranged by size:
981 275 52
SacII CC_GC'GG
Cuts at: 0 262 1308
Size: 262 1046
ScaI AGT'ACT
Cuts at: 0 181 328 1308
Size: 181 147 980
Fragments arranged by size:
980 181 147
SfcI C'TryA_G
Cuts at: 0 1115 1308
Size: 1115 193
SfoI GGC'GCC
Cuts at: 0 51 1308
Size: 51 1257
SmaI CCC'GGG
Cuts at: 0 70 1308
Size: 70 1238
SmlI C'TyrA_G
Cuts at: 0 142 322 861 1255 1308
Size: 142 180 539 394 53
Fragments arranged by size:
539 394 180 142 53
SphI G_CATG'C
Cuts at: 0 471 1308
Size: 471 837
StuI AGG'CCT
Cuts at: 0 205 1308
Size: 205 1103
StyI C'CwwG_G
Cuts at: 0 551 998 1308
Size: 551 447 310
Fragments arranged by size:
551 447 310
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 882 1086 1308
Size: 882 204 222
Fragments arranged by size:
882 222 204
TatI w'GTAC_w
Cuts at: 0 179 326 1308
Size: 179 147 982
Fragments arranged by size:
982 179 147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 456 498 1308
Size: 456 42 810
Fragments arranged by size:
810 456 42
XhoI C'TCGA_G
Cuts at: 0 142 322 1308
Size: 142 180 986
Fragments arranged by size:
986 180 142
XmaI C'CCGG_G
Cuts at: 0 68 1308
Size: 68 1240
XmnI GAAnn'nnTTC
Cuts at: 0 85 1308
Size: 85 1223
Enzymes that do cut:
AclI AcuI AhdI AlwNI ApaLI ApoI AscI AvaI
BanI BanII BbeI BbsI BciVI BglI BglII Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BstXI
BstYI BtgI BtsI EaeI EagI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I HindIII KasI MmeI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PflMI PfoI PspXI SacI SacII ScaI SfcI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BclI BfrBI BlpI BplI Bpu10I
BsiWI BsmBI BspEI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EarI EcoNI EcoRV FseI FspI FspAI HincII
HpaI KpnI MfeI MluI MscI NdeI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI TstI Tth111I XbaI ZraI