Restriction map for
pFN21AE3978
(Linear) (Six-base) MAPSORT of: KIEE3978.seq Check: 7955 from: 1 to: 1176
KIEE3978.orf
With 182 enzymes: SgfI *
March 22, 2012 17:02 ..
AccI GT'mk_AC
Cuts at: 0 40 1176
Size: 40 1136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 764 874 1176
Size: 105 659 110 302
Fragments arranged by size:
659 302 110 105
AhdI GACnn_n'nnGTC
Cuts at: 0 249 1176
Size: 249 927
ApoI r'AATT_y
Cuts at: 0 91 301 307 403 628 1176
Size: 91 210 6 96 225 548
Fragments arranged by size:
548 225 210 96 91 6
AseI AT'TA_AT
Cuts at: 0 113 974 1176
Size: 113 861 202
Fragments arranged by size:
861 202 113
AvaI C'yCGr_G
Cuts at: 0 5 1176
Size: 5 1171
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 902 935 1176
Size: 902 33 241
Fragments arranged by size:
902 241 33
BanI G'GyrC_C
Cuts at: 0 200 540 1176
Size: 200 340 636
Fragments arranged by size:
636 340 200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 594 628 1176
Size: 594 34 548
Fragments arranged by size:
594 548 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 399 1176
Size: 399 777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 650 1176
Size: 50 600 526
Fragments arranged by size:
600 526 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1002 1112 1176
Size: 959 43 110 64
Fragments arranged by size:
959 110 64 43
BsiHKAI G_wGCw'C
Cuts at: 0 789 1119 1176
Size: 789 330 57
Fragments arranged by size:
789 330 57
BsmI GAATG_Cn'
Cuts at: 0 327 1148 1176
Size: 327 821 28
Fragments arranged by size:
821 327 28
Bsp1286I G_dGCh'C
Cuts at: 0 789 1119 1176
Size: 789 330 57
Fragments arranged by size:
789 330 57
BsrDI GCAATG_nn'
Cuts at: 0 353 1176
Size: 353 823
Bsu36I CC'TnA_GG
Cuts at: 0 205 1176
Size: 205 971
BtsI GCAGTG_nn'
Cuts at: 0 990 1176
Size: 990 186
ClaI AT'CG_AT
Cuts at: 0 369 1176
Size: 369 807
DraI TTT'AAA
Cuts at: 0 399 633 753 1176
Size: 399 234 120 423
Fragments arranged by size:
423 399 234 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 105 650 764 874 1176
Size: 50 55 545 114 110 302
Fragments arranged by size:
545 302 114 110 55 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 1068 1100 1176
Size: 235 32 801 32 76
Fragments arranged by size:
801 235 76 32 32
HindIII A'AGCT_T
Cuts at: 0 460 872 1176
Size: 460 412 304
Fragments arranged by size:
460 412 304
MslI CAynn'nnrTG
Cuts at: 0 1062 1176
Size: 1062 114
MspA1I CmG'CkG
Cuts at: 0 275 826 1176
Size: 275 551 350
Fragments arranged by size:
551 350 275
PflMI CCAn_nnn'nTGG
Cuts at: 0 1144 1176
Size: 1144 32
PstI C_TGCA'G
Cuts at: 0 607 831 1176
Size: 607 224 345
Fragments arranged by size:
607 345 224
PvuII CAG'CTG
Cuts at: 0 275 826 1176
Size: 275 551 350
Fragments arranged by size:
551 350 275
SfcI C'TryA_G
Cuts at: 0 603 827 1176
Size: 603 224 349
Fragments arranged by size:
603 349 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 992 1176
Size: 133 859 184
Fragments arranged by size:
859 184 133
TatI w'GTAC_w
Cuts at: 0 987 1176
Size: 987 189
XmnI GAAnn'nnTTC
Cuts at: 0 1109 1176
Size: 1109 67
Enzymes that do cut:
AccI AcuI AhdI ApoI AseI AvaI BaeI BanI
BcgI BmrI BpmI BsgI BsiHKAI BsmI Bsp1286I BsrDI
Bsu36I BtsI ClaI DraI Eco57MI Hin4I HindIII MslI
MspA1I PflMI PstI PvuII SfcI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BamHI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI CspCI DraIII DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI